刺络泻血对高尿酸血症大鼠血尿酸及相关酶活性的影响

发布时间：2018-05-01 08:35

本文选题：刺络泻血 + 高尿酸血症　；参考：《世界中医药》2017年03期

【摘要】：目的:观察刺络泻血疗法对高尿酸血症模型大鼠血尿酸及相关酶活性的影响并探讨其机制。方法:将40只清洁级SD大鼠分为正常组、模型组、别嘌呤醇组及刺络泻血组,每组10只,以腺嘌呤、盐酸乙胺丁醇混合液灌胃造模。第4周造模成功后,别嘌呤醇组按照0.03 g/(kg·d)的剂量给大鼠灌胃别嘌呤醇,2次/周;刺络泻血组选取大鼠委中穴及足三里穴穴位附近的血络,出血0.3~0.5 m L,至第8周。第9周后腹主动脉取血,采用磷钨酸法测定血尿酸,采用酶比色法测定黄嘌呤氧化酶(XOD)和腺苷脱氨酶(ADA)的含量。结果:实验结束后,与正常组相比,模型组SUA、ADA、XOD含量均显著增高(P0.05),差异有统计学意义;与模型组相比,别嘌呤醇组SUA、XOD含量显著降低(P0.05),刺络泻血组SUA、ADA、XOD含量差异均有统计学意义(P0.05)。结论:刺络泻血疗法可以有效抑制XOD、ADA活性,降低高尿酸血症大鼠SUA水平。
[Abstract]:Aim: to observe the effect of pricking collaterals and purging blood on the activity of serum uric acid and related enzymes in hyperuricemia rats and to explore its mechanism. Methods: forty SD rats of clean grade were divided into normal group, model group, allopurinol group and prickly purging blood group with 10 rats in each group. After the model was established successfully at the 4th week, the rats in the allopurinol group were given intragastric administration of allopurinol twice a week according to the dose of 0.03 g/(kg d, and the blood collaterals near the acupoints of Weizhong and Zusanli of the rats in the pricking collaterals were selected and bleeding was 0.3 ~ 0.5 mL to the 8th week. After 9 weeks, the contents of serum uric acid, xanthine oxidase (XODO) and adenosine deaminase (ADA) were determined by phosphotungstic acid method, xanthine oxidase XODs and adenosine deaminase (ADA). Results: after the end of the experiment, compared with the normal group, the XOD content of the model group was significantly higher than that of the normal group, the difference was statistically significant, compared with the model group, the XOD content of SUAA XOD in the allopurinol group was significantly lower than that in the model group, and the difference of the XOD content in the SUAA ADAXOD group in the prickly purge blood group was significantly higher than that in the model group. Conclusion: pricking collaterals and purging blood therapy can effectively inhibit the activity of ADA and decrease the level of SUA in hyperuricemia rats.
【作者单位】：北京中医药大学;
【基金】：教育部人文社科研究项目(编号:15YJAZH117)
【分类号】：R245

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