电针治疗小鼠酒精戒断期抑郁状态和机制研究

发布时间：2018-05-14 17:37

本文选题：电针 + 酒精　；参考：《新乡医学院》2017年硕士论文

【摘要】：目的针灸是祖国传统医学的瑰宝,在临床多种疾病的治疗和预防中有着广泛的应用,电针的发明为现代医学研究针灸的治疗作用和机制提供了科学的手段。本实验研究了2/100Hz电针能否治疗小鼠酒精依赖戒断期的抑郁状态,能否翻转小鼠酒精依赖戒断期海马脑区脑源性神经营养因子和神经发生的减少。方法把48只小鼠分为蒸馏水组、酒精依赖戒断组、束缚组和2/100Hz电针组。首先建立小鼠酒精依赖模型,采用双瓶自由选择饮酒的方法进行酒精依赖造模,接下来是14天的自然戒断,这段时间内给予电针治疗。电针组电针每天1次,8:00a.m.,频率2/100 Hz,强度分别为0.5、1.0、1.5 mA,三个强度的时间各为10min;束缚组每天1次,8:00a.m.,30min;自然戒断组动物不做任何处理,常规饲养。以上各组动物每组12只,43天时全部进行强迫游泳实验,检测动物抑郁状态,44天时选择6只给予BrdU处理(100 mg/kg,i.p.),2小时之后灌流取材,进行免疫组化实验,检测神经发生;另外6只直接处死取材,进行ELISA实验检测海马脑源性神经营养因子含量。结果1.经过28天的双瓶自由选择饮酒实验,检测小鼠的酒精偏爱率和饮酒量,我们的结果表明每只小鼠的酒精偏爱率都达到了75%以上,酒精摄入量都达到了15g/kg以上,对酒精形成了明显的偏爱,而且三组小鼠对酒精的偏爱率和酒精摄入量没有统计学差异(P0.05),同时我们每周检测小鼠体重,结果表明和蒸馏水组相比,饮酒对小鼠的体重没有影响(P0.05)。2.强迫游泳实验显示,酒精戒断14天后,和蒸馏水组相比,酒精戒断组的小鼠处于抑郁状态,强迫游泳的不动时间明显延长(P㩳0.01)。ELISA实验结果显示,酒精戒断后14天,和蒸馏水组相比,戒断组小鼠海马脑区脑源性神经营养因子的含量明显减少(P㩳0.05)。BrdU免疫组化结果显示,和蒸馏水组相比,酒精戒断组小鼠海马神经发生减少(P㩳0.05)。3.在戒断期给予小鼠2/100Hz电针治疗,强迫游泳实验中可以明显减少小鼠游泳时的不动时间(P㩳0.05),表明电针可以改善小鼠酒精依赖戒断期的抑郁状态。ELISA实验显示电针可以增加海马脑区脑源性神经营养因子的含量(P㩳0.05),BrdU免疫组化结果显示,2/100Hz电针可以促进海马神经细胞发生的增加(P㩳0.05)。结论1.双瓶自由选择饮酒14天后小鼠表现出抑郁样行为,减少小鼠海马脑源性神经营养因子含量和海马神经发生。2.2/100Hz电针可以改善酒精依赖戒断期的抑郁,增加海马脑源性神经营养因子含量和神经发生。
[Abstract]:Objective Acupuncture and moxibustion is a treasure of traditional Chinese medicine, which is widely used in the treatment and prevention of many kinds of clinical diseases. The invention of electroacupuncture provides a scientific means for modern medicine to study the therapeutic effect and mechanism of acupuncture and moxibustion. This experiment was conducted to study whether 2/100Hz electroacupuncture could treat depression in alcohol dependence withdrawal period in mice and reverse the decrease of brain-derived neurotrophic factor and neurogenesis in hippocampus of mice during alcohol dependence withdrawal period. Methods 48 mice were divided into distilled water group, alcohol dependent withdrawal group, bound group and 2/100Hz electroacupuncture group. The model of alcohol dependence in mice was established first, and the model was made with the method of double bottles of free choice. Then, the natural withdrawal of 14 days was given, and electroacupuncture was given during this period. In the electroacupuncture group, once a day, 8: 00a.m., with a frequency of 2 / 100 Hz, with the intensity of 0.5 脳 1.0 Ma and 1.5 Ma, the time of the three intensities was 10 min, and the bound group was 8: 00 a.m..30 min. once a day. The animals in the natural abstinence group did not do any treatment and were raised routinely. In each group, 12 animals in each group were subjected to forced swimming for 43 days, and 6 animals were given BrdU for 44 days. After 2 hours of BrdU treatment, the animals were perfused with 100 mg / kg i.p. for 2 hours, and the neurogenesis was detected by immunohistochemistry. The other 6 rats were killed directly and the content of brain-derived neurotrophic factor (BDNF) in hippocampus was detected by ELISA test. Result 1. After 28 days of free choice of alcohol in two bottles, the alcohol preference rate and alcohol consumption of mice were measured. Our results showed that the alcohol preference rate of each mouse was more than 75%, and the alcohol intake was above 15g/kg. There was no significant difference in alcohol preference rate and alcohol intake among the three groups. At the same time, we measured the body weight of mice every week. The results showed that alcohol consumption had no effect on the body weight of mice compared with distilled water group. The forced swimming test showed that after 14 days of alcohol withdrawal, the mice in the alcohol withdrawal group were in a depressed state compared with the distilled water group, and the immobility time of forced swimming was significantly prolonged. The results of Elisa showed that 14 days after alcohol withdrawal, compared with the distilled water group, the immobility time of forced swimming group was significantly longer than that of the distilled water group. The content of brain-derived neurotrophic factor (BDNF) in the hippocampus of abstinent group was significantly decreased. The results of immunohistochemistry showed that compared with distilled water group, the hippocampal neurogenesis of alcohol abstinent group was decreased as compared with that of distilled water group. During the abstinence period, mice were treated with 2/100Hz electroacupuncture, In forced swimming experiment, the immobility time of swimming in mice was significantly reduced, indicating that electroacupuncture could improve the depression state of mice during alcohol dependence withdrawal period. Elisa experiment showed that electroacupuncture could increase brain-derived neurotrophic factor in hippocampal brain area. The results of BrdU immunohistochemistry showed that 2 / 100 Hz electroacupuncture could promote the increase of hippocampal neurogenesis. Conclusion 1. After 14 days of drinking, the mice showed depressive behavior. Reducing the content of brain-derived neurotrophic factor in hippocampus and hippocampal neurogenesis. 2.2 / 100 Hz electroacupuncture could improve depression in alcohol dependence withdrawal period. Increase the content of brain-derived neurotrophic factor and neurogenesis in hippocampus.
【学位授予单位】：新乡医学院
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R245

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