按部选穴针刺对糖尿病胃轻瘫大鼠Ghrelin影响的研究

发布时间：2018-05-15 00:10

本文选题：腧穴配伍 + 糖尿病胃轻瘫　；参考：《湖南中医药大学》2016年硕士论文

【摘要】：目的:通过研究不同按部选穴针刺治疗糖尿病胃轻瘫大鼠的疗效差异,探讨按部选穴是影响腧穴配伍效应的主要因素之一。方法:将60只雄性SPF级SD大鼠,适应性喂养1周后,随机分为空白对照组、模型组、足三里+中脘组、足三里+内关组、足三里+非经非穴组,每组12只。除外空白组,其余48只大鼠运用链脲佐菌素-普通喂养法建立糖尿病胃轻瘫大鼠模型,于第10周末开始针刺治疗,治疗4周后处死,取血清、下丘脑、胃底组织。运用ELISA检测血清中Ghrelin含量;应用免疫组化检测胃底组织及下丘脑组织中Ghrelin蛋白含量;应用Real-time PCR检测胃底组织、下丘脑Ghrelin、GHSR mRNA的表达。结果:1.与空白组相比,模型组的食量、下丘脑及胃底ghrelin、GHSR mRNA表达明显升高,小肠推进率、血清中Ghrelin含量、下丘脑及胃底Ghrelin灰度值明显降低(P0.05),表明造模成功;2.与模型组相比,足三里+中脘组、足三里+内关组、足三里+非经非穴组下丘脑及胃底Ghrelin、GHSR mRNA表达明显降低,小肠推进率、血清中Ghrelin含量、下丘脑及胃底Ghrelin灰度值表达明显升高(P0.05),表明三个治疗组均能有效治疗DGP。3.与足三里+中脘组相比,足三里+内关组、足三里+非经非穴组饮食量增多,足三里+内关组胃底组织及下丘脑组织的Ghrelin、GHSR mRNA表达均升高(P0.05),足三里+内关组血清Ghrelin含量明显降低(P0.05),表明足三里+中脘组在调节DGP大鼠Ghrelin方面优于足三里+内关组。结论:针刺改善糖尿病胃轻瘫胃肠动力与大鼠血清Ghrelin含量、胃底组织及下丘脑中Ghrelin、GHSR表达有关,表明配伍局部腧穴优于配伍远端腧穴,按部选穴是影响腧穴配伍效应的主要因素之一。
[Abstract]:Objective: to study the difference of therapeutic effect of different acupoints selection in treating diabetic rats with gastroparesis, and to explore that the selection of acupoints is one of the main factors affecting the compatibility of acupoints. Methods: sixty male SPF SD rats were randomly divided into control group, model group, Zusanli Zhongwan group, Zusanli Neiguan group and Zusanli non-meridian group (12 rats in each group) after one week of adaptive feeding. Except the blank group, the other 48 rats were treated with streptozotocin-common feeding method to establish diabetic gastroparesis model. Acupuncture treatment began at the end of the 10th week. After 4 weeks of treatment, the rats were killed and serum, hypothalamus and gastric fundus were collected. The content of Ghrelin in serum was detected by ELISA, the content of Ghrelin protein in gastric fundus and hypothalamus was detected by immunohistochemistry, and the expression of GHSR mRNA in hypothalamus was detected by Real-time PCR. The result is 1: 1. Compared with the blank group, the food intake, the expression of GHSR mRNA in hypothalamus and stomach floor of the model group were significantly increased, the small intestinal propulsive rate, the content of Ghrelin in serum and the gray value of Ghrelin in hypothalamus and stomach floor were significantly decreased in the model group, indicating that the model was successful. Compared with the model group, the expression of GHSR mRNA in the hypothalamus and stomach floor of Zusanli Zhongwan group, Zusanli Neiguan group and Zusanli Feijing group was significantly decreased, the small intestinal propulsion rate and the content of Ghrelin in serum were significantly decreased. The expression of Ghrelin in hypothalamus and gastric fundus was significantly increased (P 0.05), which indicated that all the three treatment groups could effectively treat DGP.3. Compared with Zusanli Zhongwan group, Zusanli Neiguan group, Zusanli Feijing group increased diet, The expression of GHSR mRNA in gastric fundus and hypothalamus in Zusanli Neiguan group was significantly higher than that in Zusanli Neiguan group. The serum Ghrelin content in Zusanli Neiguan group was significantly lower than that in Zusanli Neiguan group, indicating that Zusanli Zhongwan group was superior to Zusanli Neiguan group in regulating Ghrelin in DGP rats. Conclusion: the improvement of gastrointestinal motility of diabetic gastroparesis by acupuncture is related to the content of Ghrelin in serum, the expression of ghrelin GHSR in gastric floor tissue and hypothalamus, which indicates that the compatibility of local acupoints is better than that of distal acupoints. Selection of acupoints by the ministry is one of the main factors affecting the compatibility of acupoints.
【学位授予单位】：湖南中医药大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：R245

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