肺瘤平膏及其拆方含药血清对体外负载肿瘤抗原树突状细胞与T细胞形成免疫突触的影响

发布时间：2018-05-15 00:26

本文选题：肿瘤 + 肺瘤平膏　；参考：《中医杂志》2017年01期

【摘要】：目的探讨肺瘤平膏抗肿瘤的可能免疫调节机制。方法肺瘤平膏拆方分为解毒方、益气方、益气解毒方,分别制备含药血清。应用扫描电镜及激光共聚焦显微镜观察成熟树突状细胞与T细胞体外相互作用动态过程。树突状细胞培养设为空白组,将负载肿瘤抗原树突状细胞分为负载组、解毒组、益气组、益气解毒组、全方组和地米低、中、高剂量组,每组设定106/106、106/105、105/106、105/105(树突状细胞/T细胞)4个浓度配比。细胞培养第2天,解毒组、益气组、益气解毒组、全方组吸取相应中药血清1 ml加入细胞中,地米低、中、高剂量组分别加入浓度为37.5、75.0、150.0 mg/ml地塞米松磷酸钠注射液1 ml,空白组、负载组加入不完全RPMI-1640培养液1 ml。于培养第8天,比较各组在混合培养3、6 h时T细胞数和免疫突触数。结果体外负载肿瘤抗原树突状细胞与T细胞体外混合培养30 min时观察到免疫突触形成。3 h时T细胞发生了明显增殖,6 h后随着免疫突触的减少,细胞分布逐渐趋于均匀。全方组在3、6 h时T细胞数和免疫突触数均明显高于其余各组(P0.05)。各给药组在106/106配比下免疫突触数均明显高于其他配比(P0.01)。各组细胞在不同配比下培养6 h与本组3 h时免疫突触数比较差异均无统计学意义(P0.05)。结论肺瘤平膏具有一定调节免疫突触形成及促进负载肿瘤抗原树突状细胞刺激T细胞增殖作用,且全方效果优于其拆方。
[Abstract]:Objective to explore the possible immunomodulatory mechanism of Feiyangping ointment in anti-tumor. Methods Feiyangping ointment was divided into jiedu prescription and Yiqi jiedu prescription. The dynamic process of interaction between mature dendritic cells and T cells in vitro was observed by scanning electron microscope and laser confocal microscope. Dendritic cells were divided into three groups: loading group, detoxifying group, supplementing qi group, whole prescription group and low, medium and high dose groups. Each set a ratio of 106 / 106106 / 105105 / 106105 (dendritic cell / T cell). On the second day of cell culture, the detoxification group, Yiqi detoxification group, and the whole prescription group absorbed 1ml of serum from traditional Chinese medicine to be added into the cells. The concentration of dexamethasone sodium phosphate injection was 37.5 mg/ml 75.0150.0 mg/ml in the low, medium and high dose groups, respectively, and the blank group was blank group. The loading group added 1 ml of incomplete RPMI-1640 medium. On the 8th day, the number of T cells and the number of immunosynaptic synapses were compared. Results after 30 min of mixed culture of dendritic cells and T cells loaded with tumor antigen in vitro, it was observed that T cells proliferated significantly at 3. 3 h after immunosynaptic formation. After 6 h, the distribution of T cells became more and more uniform with the decrease of immune synapses. The number of T cells and the number of immunosynaptic synapses in the whole prescription group were significantly higher than those in the other groups at 3h. The number of immunosynaptic synapses in each group at 106 / 106 was significantly higher than that in other groups (P 0.01). There was no significant difference in the number of immunosynaptic synapses between the cells cultured in different proportions for 6 h and 3 h in this group (P 0.05). Conclusion HYP can regulate immune synapse formation and promote T cell proliferation stimulated by dendritic cells loaded with tumor antigen.
【作者单位】：中国人民解放军第三0六医院;中国科学院国家纳米中心;中国中医科学院广安门医院;
【基金】：北京市自然科学基金(7122156)
【分类号】：R273

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