温针灸对兔膝骨性关节炎模型软骨细胞中c-Jun氨基末端激酶信号通路的影响

发布时间：2018-05-16 09:52

本文选题：温针灸 + 膝骨关节炎　；参考：《中国中西医结合杂志》2017年11期

【摘要】：目的观察温针灸治疗兔膝骨性关节炎(KOA)模型软骨中c-Jun氨基末端激酶(JNK)信号通路及基质金属蛋白酶(MMP)-1、MMP-13的表达,为温针灸治疗KOA提供现代医学理论支持。方法 40只新西兰大耳兔随机选择30只采用右膝关节伸直位石膏固定制备KOA模型,另10只为正常对照组。固定6周后行X线检查确定造模成功后随机分为温针灸组、双氯芬酸钠组、模型组,每组10只。温针灸组选取足三里、鹤顶、内膝眼穴给予温针灸治疗,每天1次,15min/次,治疗2周;双氯芬酸钠组按15mg/kg灌胃,每天1次,治疗2周;模型组及正常对照组不予任何干预,正常饲养。采用免疫组织化学法、RT-PCR、免疫蛋白印迹法(Western Blot)检测软骨中JNK1、JNK2、MMP-1、MMP-13的表达。结果与正常对照组比较,模型组关节软骨中MMP-1、MMP-13、JNK1、JNK2mRNA及蛋白表达升高(P0.05),且温针灸组、双氯芬酸钠组关节软骨中MMP-1、MMP-13、JNK1、JNK2mRNA及蛋白表达较模型组降低(P0.05),同时温针灸组MMP-1、MMP-13mRNA蛋白表达及JNK1、JNK2 mRNA表达较双氯芬酸钠组降低更为明显(P0.05)。结论温针灸可抑制KOA软骨细胞中JNK1、JNK2、MMP-1、MMP-13的表达,温针灸治疗KOA可能是通过激活JNK信号通路,抑制MMP-1、MMP-13的表达而达到治疗作用。
[Abstract]:Objective To observe the signal pathway of c-Jun amino terminal kinase (JNK) signal and the expression of matrix metalloproteinase (MMP) -1 and MMP-13 in the cartilage of rabbit knee osteoarthritis (KOA) model with warm acupuncture and moxibustion, and to provide modern medical theory support for the treatment of KOA by warm acupuncture and moxibustion. Methods 40 New Zealand rabbits were randomly selected and 30 were fixed by plaster fixation of the right knee joint. The KOA model and the other 10 were in the normal control group. After 6 weeks, the X-ray examination was used to determine the success of the model, and then randomly divided into the warm acupuncture group, the diclofenac sodium group and the model group, 10 in each group. The warm acupuncture group selected the Zusanli, the crane top and the inner knee hole to give warm acupuncture and moxibustion treatment, 1 times a day, and the treatment for 2 weeks, and the diclofenac sodium group was gavage by 15mg/kg, 1 times a day. After 2 weeks of treatment, the model group and the normal control group had no intervention and normal feeding. The expression of JNK1, JNK2, MMP-1, MMP-13 in cartilage was detected by immunohistochemistry, RT-PCR and Western Blot. Results compared with the normal control group, the MMP-1, MMP-13, JNK1, JNK2mRNA and protein expression in the model group were increased (P0.05). In the warm acupuncture group, the expression of MMP-1, MMP-13, JNK1, JNK2mRNA and protein in the articular cartilage of diclofenac sodium group was lower than that in the model group (P0.05). At the same time, the expression of MMP-1, MMP-13mRNA protein and JNK1, JNK2 mRNA expression in the warm acupuncture group were more obvious than that of the diclofenac sodium group (P0.05). Conclusion warm acupuncture and moxibustion can inhibit the KOA cartilage cells. The expression of KOA may be mediated by the activation of JNK signaling pathway and the inhibition of MMP-1 and MMP-13 expression.

【作者单位】：宁夏医科大学总医院中医骨伤科;
【基金】：国家自然科学基金资助项目(No.81260545)
【分类号】：R245;R-332

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