彝药劳伤药对佐剂性关节炎大鼠滑膜病变及NF-κB蛋白表达的影响

发布时间：2018-05-19 07:00

本文选题：核转录因子-κB + 白细胞介素　；参考：《中华中医药学刊》2017年04期

【摘要】：目的:观察劳伤药乙醇浸膏对佐剂性关节炎(AA)大鼠滑膜病变及滑膜细胞核转录因子(NF-κB)蛋白表达的影响,探讨其治疗类风湿性关节炎(RA)可能的作用机制。方法:60只SD大鼠随机分成6组:空白组,模型组,尼美舒利组,劳伤药高、中、低剂量组,采用大鼠右后足趾皮内注射0.l m L弗氏完全佐剂,致炎形成AA模型,给药28 d后处死大鼠,HE染色观察踝关节滑膜组织形态学变化,酶联免疫吸附法测定大鼠血清IL-1β、IL-6、IL-10、TNF-α、NO、PGE_2含量,免疫组化法检测滑膜组织中NF-κB的蛋白表达水平。结果:与模型组比较,劳伤药组(5 g/kg)明显减轻滑膜细胞的增生,减少滑膜层和滑膜下层淋巴细胞浸润,明显降低AA大鼠血清中IL-1β、IL-6、TNF-α、NO、PGE_2的含量和升高IL-10的含量,降低关节滑膜组织NF-κB的蛋白含量。结论:劳伤药能下调滑膜组织中NF-κB的蛋白表达,从而促进细胞凋亡,改善滑膜病变。
[Abstract]:Aim: to observe the effects of alcohol extract on synovial lesions and the expression of NF- 魏 B protein in synovial nuclei of rats with adjuvant arthritis (AA) and to explore the possible mechanism of its treatment of rheumatoid arthritis (RA). Methods Sixty Sprague-Dawley rats were randomly divided into 6 groups: blank group, model group, nimesulide group, high, middle and low dose groups. AA model was induced by intradermal injection of 0.1 ml Freund's complete adjuvant into the right hind toe of rats. After 28 days of administration, the rats were killed to observe the histomorphologic changes of the synovial membrane of the ankle joint by HE staining. The serum levels of IL-1 尾 -IL-6, IL-10, TNF- 伪 and NOPGE2 in the synovium of the rats were determined by enzyme-linked immunosorbent assay, and the protein expression of NF- 魏 B in the synovial tissue was detected by immunohistochemical method. Results: compared with the model group, 5 g 路kg ~ (-1) of the drug significantly reduced the proliferation of synovial cells, the infiltration of lymphocytes in synovium and subsynovium, and the content of IL-1 尾 -IL-6 TNF- 伪 no PGE2 and increased the content of IL-10 in serum of AA rats. The protein content of NF- 魏 B in synovial tissue was decreased. Conclusion: the drug can down-regulate the expression of NF- 魏 B protein in synovial tissue, thus promote cell apoptosis and improve synovial lesions.
【作者单位】：西南交通大学生命科学与工程学院;凉山彝族自治州第二人民医院;
【基金】：凉山州科技局应用技术研究开发项目(14YYJS0045)
【分类号】：R29

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