不同刺灸法对大鼠功能性便秘干预作用的比较研究

发布时间：2018-05-23 06:50

本文选题：功能性便秘 + 毫针　；参考：《中国针灸》2017年05期

【摘要】：目的:比较毫针刺法、电针和艾灸对大鼠功能性便秘的干预作用。方法:将60只雄性SD大鼠按随机数字表法分为对照组(8只)、模型组(11只)、药物组(8只)、毫针组(11只)、电针组(11只)和艾灸组(11只)。除对照组外,余组均以盐酸洛哌丁胺混悬液连续灌胃6d制备功能性便秘模型,同时对照组以等体积饮用水灌胃。毫针组、电针组和艾灸组均于每日灌胃后1h分别给予毫针针刺、电针干预、艾条温和灸,均取"天枢""上巨虚";药物组予西沙必利混悬液灌胃,均每天干预1次,连续6d。计量各组大鼠干预最后1日24h摄食量、粪便量及粪便含水量,采用炭餐指示剂进行肠推进率试验,应用苏木精-伊红染色和阿利新蓝染色观察结肠组织结构及黏膜层酸性黏液,采用实时荧光定量PCR(real-time PCR)法检测结肠组织干细胞因子(stem cell factor,SCF)、c-kit mRNA的表达。结果:与对照组比较,模型组24h摄食量、粪便量均减少(均P0.01),粪便含水量、肠推进率均降低(均P0.01);与模型组比较,药物组、毫针组、电针组24h粪便量、粪便含水量均增加(P0.05,P0.01),艾灸组差异无统计学意义(P0.05),各干预组肠推进率均升高(均P0.01);与药物组比较,毫针组、电针组和艾灸组24h摄食量、粪便量差异均无统计学意义(均P0.05),粪便含水量仅艾灸组降低(P0.01),毫针组、艾灸组肠推进率均低于药物组(均P0.01),而电针组与药物组差异无统计学意义(P0.05);艾灸组粪便含水量低于毫针组、电针组(均P0.01),毫针组、艾灸组肠推进率均低于电针组(均P0.01)。HE染色显示各组大鼠结肠组织结构正常、完整,各组间无明显差异。阿利新蓝染色显示黏膜层黏液物质,与对照组比较,模型组酸性黏液明显减少;与模型组比较,药物组、毫针组、电针组和艾灸组均有不同程度增加。与对照组比较,模型组结肠组织SCF、c-kit mRNA表达均降低(均P0.05);与模型组比较,药物组、毫针组、电针组和艾灸组SCF、c-kit mRNA表达均升高(均P0.05);毫针组、电针组c-kit mRNA表达均低于艾灸组(均P0.05)。结论:毫针刺法、电针和艾灸均对大鼠功能性便秘具有不同程度的良性调整作用,以电针作用最佳,毫针刺法次之。
[Abstract]:Objective: to compare the interventional effects of Milliform Acupuncture, electroacupuncture and moxibustion on functional constipation in rats. Methods: sixty male Sprague-Dawley rats were randomly divided into control group (n = 8), model group (n = 11), drug group (n = 8), filiform group (n = 11), electric acupuncture group (n = 11) and moxibustion group (n = 11). Except the control group, the functional constipation model was established by continuous oral administration of loperamide hydrochloride suspension for 6 days, while the control group was perfused with the same volume of drinking water. The patients in the filiform acupuncture group, electroacupuncture group and moxibustion group were treated with filiform acupuncture, electroacupuncture intervention, moxa mild moxibustion, "Tianshu" and "Shangzuxu", respectively, and the drug group were given cisapride suspension once a day for 6 days. The feeding amount, fecal quantity and fecal water content of rats in each group were measured at 24 h after intervention on the last day. The intestinal propulsion rate was tested with charcoal meal indicator. The colonic tissue structure and mucous mucus in mucous membrane were observed by hematoxylin eosin staining and alimine blue staining. The expression of stem cell factor (SCFK) c-kit mRNA in colon tissue was detected by real-time fluorescence quantitative PCR(real-time. Results: compared with the control group, 24 h food intake and fecal quantity in the model group were decreased (all P 0.01), the fecal water content and intestinal propulsive rate were all decreased (all P 0.01), and compared with the model group, 24 h fecal quantity was decreased in the drug group, the filiform needle group and the electroacupuncture group. The fecal water content was increased in both groups, but there was no significant difference between the moxibustion group and the moxibustion group, but the intestinal propulsive rate in each intervention group was all increased (all P 0.01), compared with the drug group, the intakes of the millimeter needle group, the electroacupuncture group and the moxibustion group were 24 h, and that of the moxibustion group was higher than that of the control group. There was no significant difference in fecal quantity (P 0.05). The fecal water content in moxibustion group was lower than that in moxibustion group (P 0.01), while that in the filiform needle group was lower than that in the control group. The intestinal propulsive rate of moxibustion group was lower than that of drug group (all P 0.01), but there was no significant difference between electroacupuncture group and drug group, while the fecal water content in moxibustion group was lower than that in millineedle group, and that in electroacupuncture group was lower than that in millineedle group. The intestinal propulsive rate of moxibustion group was lower than that of electroacupuncture group (P0.01).HE staining showed that the colonic tissue structure of each group was normal and intact, and there was no significant difference between each group. Compared with the control group, the acid mucus in the model group decreased significantly, and the drug group, the millimeter needle group, the electroacupuncture group and the moxibustion group increased in varying degrees compared with the model group. Compared with the control group, the expression of SCFc-kit mRNA in colonic tissue of model group was lower than that in control group (P0.05), and the expression of SCFc-kit mRNA in drug group, millimeter acupuncture group, electroacupuncture group and moxibustion group was higher than that in model group (all P 0.05), and the expression of c-kit mRNA in acupuncture group and electroacupuncture group was lower than that in moxibustion group (P 0.05). Conclusion: the effect of acupuncture, electroacupuncture and moxibustion on functional constipation in rats was better than that of acupuncture.
【作者单位】：河北中医学院针灸推拿学院;
【基金】：河北省高等学校青年拔尖人才计划项目:BJ 2014047 河北中医学院博士科研基金项目:BSZ 2015009
【分类号】：R245

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