藏药波棱瓜子总木脂素对大鼠肝星状细胞增殖与凋亡的影响

发布时间：2018-05-31 11:46

本文选题：波棱瓜子总木脂素 + 大鼠肝星状细胞　；参考：《中草药》2017年14期

【摘要】：目的通过研究藏药波棱瓜子总木脂素(TLHPS)对大鼠肝星状HSC-T6细胞的增殖抑制作用及诱导其凋亡的作用,进一步探讨TLHPS抗肝纤维化的作用机制。方法实验分为对照组,TLHPS 10、20、30、40μg/mL组,阳性药秋水仙碱0.1μg/mL组。用加入各组药物的培养基分别培养HSC-T6细胞24、48、72 h,采用CCK8法检测各组细胞24、48、72 h的增殖抑制率,流式细胞术检测细胞凋亡率及细胞周期,Western blotting法检测凋亡蛋白Bcl-2、Bax、核转录因子-κB(NF-κB)的表达水平。结果与对照组相比,TLHPS 10、20、30、40μg/mL组对HSC-T6细胞24、48、72 h时的增殖抑制率明显增高(P0.01),且各组HSC-T6细胞早期凋亡率与晚期凋亡率明显升高(P0.05,0.01),G0/G1期细胞数明显增多(P0.01),G2/M期细胞数无明显差异(P0.05),S期细胞数明显减少(P0.01)。Western blotting结果显示,与对照组相比,TLHPS10、20、30、40μg/mL组Bcl-2蛋白表达明显降低(P0.05,0.01),TLHPS 40μg/mL组Bax蛋白表达明显升高(P0.01),TLHPS 20、30、40μg/mL组NF-κB表达明显降低(P0.01)。结论 TLHPS抗肝纤维化的作用机制可能是通过降低Bcl-2、NF-κB的表达抑制肝星状细胞增殖并诱导其凋亡。
[Abstract]:Objective to investigate the inhibitory effect of Tibetan medicine (TLHPS) on the proliferation and apoptosis of rat hepatic stellate HSC-T6 cells, and to explore the mechanism of anti-hepatic fibrosis induced by TLHPS. Methods the experiment was divided into two groups: the control group (TLHPS 1020), the control group (30 渭 g/mL), and the positive colchicine group (0.1 渭 g/mL). HSC-T6 cells were cultured on the medium supplemented with each group for 24 ~ 48 ~ 72 h, respectively. The proliferation inhibition rate of each group was detected by CCK8 assay for 24 ~ 48 ~ (8) h. The apoptotic rate was detected by flow cytometry and the expression of apoptotic protein Bcl-2 and NF- 魏 B were detected by Western blotting. Results compared with the control group, the proliferation inhibition rate of TLHPS 1020 + 30 + 40 渭 g/mL on HSC-T6 cells was significantly increased at 24: 4872 h, and the early apoptosis rate and late apoptosis rate of HSC-T6 cells in each group were significantly higher than those in the control group. There was no significant difference in the number of cells in the G _ 2 / M phase of P0.01T _ 0 / G _ (1) and in the G _ 2 / M phase of HSC-T6 cells in each group. The results of Western blotting showed that the number of cells decreased significantly. Compared with the control group, the expression of Bcl-2 protein in the TLHPS1020 / 3040 渭 g/mL group was significantly lower than that in the P0.05HPS40 渭 g/mL group and the Bax protein expression in the TLHPS40 渭 g/mL group was significantly higher than that in the control group. The expression of NF- 魏 B in the TLHPS203040 渭 g/mL group was significantly lower than that in the P0.05-TLHPS40 渭 g/mL group, and the expression of NF- 魏 B in the TLHPS3040 渭 g/mL group decreased significantly. Conclusion TLHPS may inhibit the proliferation and induce apoptosis of hepatic stellate cells by decreasing the expression of Bcl-2nf- 魏 B.
【作者单位】：西南民族大学药学院;
【基金】：国家自然科学基金资助项目(81274168,81573563) 四川省中医药管理局科研项目(2016C062)
【分类号】：R29

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