电针预处理对脑缺血再灌注损伤大鼠神经功能和缺血半暗区Tolls样受体4、核转录因子κB蛋白的影响

发布时间：2018-06-01 20:06

本文选题：脑缺血再灌注损伤 + 电针　；参考：《中国康复理论与实践》2017年07期

【摘要】：目的探讨电针预处理对大鼠脑缺血再灌注损伤后神经功能的影响及其机制。方法雄性Sprague-Dawley大鼠36只随机分为假手术组(n=12)、模型组(n=12)和电针预处理组(n=12)。后两组线栓法制备大鼠脑缺血2 h再灌注模型。电针预处理组在缺血前给予电针百会干预2周。再灌注24 h后,采用改良神经功能缺损评分进行评定,HE染色观察缺血侧脑组织脑损伤程度,Western blotting检测脑缺血半暗区Tolls样受体4(TLR4)、核转录因子κB(NF-κB)蛋白表达。结果与模型组比较,电针预处理组神经功能缺损评分降低(P0.05),脑组织损伤程度减轻,缺血半暗区TLR4、NF-κB蛋白表达下调(P0.05)。结论电针预处理通过下调缺血半暗区TLR4、NF-κB蛋白表达,诱导脑保护作用,降低炎性损伤程度,改善脑缺血再灌注损伤后大鼠的神经功能。
[Abstract]:Objective to investigate the effect and mechanism of electroacupuncture preconditioning on nerve function after cerebral ischemia reperfusion injury in rats. Methods Thirty-six male Sprague-Dawley rats were randomly divided into sham operation group (n = 12), model group (n = 12) and electroacupuncture preconditioning group (n = 12). The model of cerebral ischemia 2 h reperfusion was established in the latter two groups. The electroacupuncture preconditioning group was given electroacupuncture for 2 weeks before ischemia. After 24 hours of reperfusion, the degree of brain injury in ischemic brain tissue was evaluated by HE staining. The expression of Tolls like receptor 4 (TLR4) and nuclear transcription factor 魏 B (NF-魏 B) in ischemic penumbra was detected by Western blotting. Results compared with the model group, the nerve function defect score of electroacupuncture preconditioning group was decreased (P 0.05), the degree of brain tissue injury was alleviated, and the expression of TLR4 / NF- 魏 B protein in ischemic penumbra was down-regulated (P 0.05). Conclusion electroacupuncture preconditioning can reduce the degree of inflammatory injury by down-regulating the expression of TLR4NF- 魏 B protein in ischemic penumbra, and improve the neurological function of rats after cerebral ischemia-reperfusion injury.
【作者单位】：黑龙江中医药大学;黑龙江中医药大学附属第二医院;
【基金】：国家自然科学基金项目(No.81503666) 黑龙江省自然科学基金项目(No.QC2015103) 哈尔滨市科技创新人才研究专项基金项目(青年后备人)(No.2014RFQGJ150) 黑龙江中医药大学领军人才计划项目(No.2012RCL02);黑龙江中医药大学研究生创新科研项目(No.yjscx2016037)
【分类号】：R245

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