补髓生血颗粒治疗慢性髓劳病的临床观察

发布时间：2018-06-04 12:32

本文选题：补髓生血颗粒 + 贫血　；参考：《黑龙江中医药大学》2016年硕士论文

【摘要】：目的：通过比较慢性再生障碍性贫血(CAA)患者应用补髓生血颗粒治疗前后的临床疗效、症状积分、外周血象和骨髓单个核细胞整合素VLA-3mRNA表达的水平,探讨补髓生血颗粒治疗CAA的临床疗效及其对VLA-3mRNA表达水平的干预,从分子生物学水平研究CAA的发病可能与造血微环境中整合素家族粘附分子表达异常的关系。方法：1、收集确诊为CAA的患者32例(2例脱落被剔除),服用补髓生血颗粒,服药6个月后,观察临床疗效、症状积分、外周血象的变化；2、应用RT-PCR法检测CAA患者治疗前后骨髓单个核细胞整合素VLA-3mRNA的表达水平。结果：1.CAA患者经6个月药物治疗后症状明显好转,中医症状积分亦有显著降低,差异有统计学意义(P0.05)；2.30例CAA患者的临床总有效率为80.0%；3.CAA患者外周血中白细胞、血红蛋白及血小板治疗后水平均较治疗前上升,差异有统计学意义(P0.05)4.CAA患者治疗前、后骨髓中单个核细胞整合素VLA-3mRNA水平与健康对照组比较均降低,治疗后水平与治疗前相比升高,差异均有统计学意义(P0.05)。结论：1.补髓生血颗粒治疗CAA疗效确切,能显著改善患者的外周血象及临床症状；2.本次研究CAA患者骨髓单个核细胞整合素VLA-3mRNA水平呈低表达状态,可能与再障的发生相关,补髓生血颗粒可能通过升高CAA患者骨髓单个核细胞整合素VLA-3mRNA水平,调整骨髓造血微环境,从而恢复造血功能。
[Abstract]:Objective: to compare the clinical effect, symptom score, peripheral blood image and the expression level of integrin VLA-3mRNA in bone marrow mononuclear cells of patients with chronic aplastic anemia before and after treatment with BUMU Shengxue granule. To explore the clinical effect of Bushi Shengxue granule in treating CAA and its intervention in VLA-3mRNA expression, and to study the relationship between the pathogenesis of CAA and the abnormal expression of integrin family adhesion molecules in hematopoietic microenvironment from the level of molecular biology. Methods: one, 32 cases of CAA were collected, 2 cases of them were removed from the body and taken the granule of nourishing the marrow and generating blood. After taking the medicine for 6 months, the clinical curative effect and symptom score were observed. The expression of integrin VLA-3mRNA in bone marrow mononuclear cells was detected by RT-PCR method before and after treatment in patients with CAA. Results 1. After 6 months of drug treatment, the symptoms of CAA patients were obviously improved, and the scores of TCM symptoms were also significantly decreased. The total clinical effective rate of 2.30 patients with CAA was 80.0 and 3.The total effective rate was 80.0%. The levels of mononuclear integrin VLA-3mRNA in bone marrow of patients with CAA before and after treatment were significantly lower than those before treatment, and the levels of integrin VLA-3mRNA in bone marrow after treatment were higher than those before treatment, and the levels of integrin in bone marrow of patients with CAA after treatment were higher than those before treatment. The difference was statistically significant (P 0.05). Conclusion 1. Busui Shengxue granule is effective in treating CAA, and can significantly improve the peripheral blood and clinical symptoms of the patients. In this study, the expression of integrin VLA-3mRNA in bone marrow mononuclear cells of patients with CAA was low, which may be related to the occurrence of aplastic anemia. Bupu Shengxue granule may adjust the hematopoietic microenvironment by increasing the VLA-3mRNA level of bone marrow mononuclear cells in patients with CAA. To restore hematopoietic function.
【学位授予单位】：黑龙江中医药大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：R259

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