PAR4、TRPV1在电针缓解肠易激综合征大鼠内脏痛敏中的作用研究

发布时间：2018-06-04 20:30

本文选题：电针 + 肠易激综合征　；参考：《中华中医药学刊》2017年09期

【摘要】：目的:观察PAR4、TRPV1在电针缓解肠易激综合征(irritable bowel syndrome,IBS)内脏痛敏中的作用,探讨针刺缓解大鼠内脏痛敏的可能机制。方法:将SPF级成年雄性Wistar大鼠32只随机分为正常对照组、模型组、电针大肠俞组、电针上巨虚组,每组8只,采用乙酸灌肠法复制IBS内脏痛敏模型。造模成功后电针治疗,每天1次,连续7 d。在电针结束后,观察各组大鼠粪便性状并根据粪便性状分级进行粪便性状的评分,检测粪便含水量和引起腹部回缩反射的最小容量阈值,采用western-blotting检测大鼠结肠组织中PAR4、TRPV1蛋白的含量。结果:模型组大鼠粪便性状评分和粪便含水量与正常对照组相比明显增加(P0.01,P0.01),电针大肠俞组、电针上巨虚组大鼠粪便性状评分(P0.01,P0.01)和粪便含水量降低(P0.05,P0.05)。模型组与正常对照组相比引起大鼠腹部回缩反射的容量阈值明显降低(P0.01),电针大肠俞和电针上巨虚后引起大鼠腹部回缩反射的容量阈值显著增加(P0.01,P0.01);模型组结肠组织PAR4蛋白的表达水平与正常对照组相比有升高的趋势,但差异无统计学意义(P0.05);与模型组相比电针上巨虚组结肠组织PAR4蛋白表达水平下降,差异有统计学意义(P0.05)。模型组结肠组织TRPV1蛋白的表达水平与正常对照组相比升高(P0.05)。与模型组相比电针大肠俞组、电针上巨虚组结肠组织TRPV1蛋白表达水平有下降的趋势。结论:电针可以有效缓解IBS内脏痛敏,其机制可能和调控IBS内脏痛敏大鼠结肠PAR4、TRPV1蛋白的表达有关。
[Abstract]:Objective: to observe the effect of PAR4 / TRPV1 on relieving visceral pain sensitivity of irritable bowel syndrome (IBSs) of irritable bowel syndrome (IBS), and to explore the possible mechanism of relieving visceral pain sensitivity by acupuncture. Methods: Thirty-two SPF grade adult male Wistar rats were randomly divided into three groups: normal control group, model group, electroacupuncture group (Dachangshu group) and Shangzhouxu group (8 rats in each group). IBS visceral pain sensitive model was induced by acetic acid enema. The model was successfully treated with electroacupuncture once a day for 7 days. After electroacupuncture, the fecal traits of rats in each group were observed and graded according to fecal traits, the fecal water content and the minimum volume threshold of abdominal reflex were measured. Western-blotting was used to detect the protein content of PAR4 and TRPV1 in rat colon. Results: the scores of fecal traits and fecal water content in the model group were significantly higher than those in the normal control group, and the scores of fecal traits were significantly increased in the Dachang Shu group and the Shangzhouxu group (P 0.01 P 0.01) and the fecal water content was decreased (P 0.05 P 0.05). Compared with the normal control group, the volume threshold of abdominal reflex in the model group was significantly lower than that in the normal control group, and that in the model group was significantly higher than that in the model group. Compared with the normal control group, the expression of PAR4 protein increased. Compared with the model group, the expression of PAR4 protein in the colon tissue of the model group decreased, and the difference was statistically significant (P 0.05). The expression of TRPV1 protein in colonic tissue of model group was higher than that of normal control group (P 0.05). Compared with the model group, the expression of TRPV1 protein in the colon tissue of the Shangjiu asthenia group showed a decreasing trend compared with the model group. Conclusion: electroacupuncture can effectively relieve visceral pain sensitivity of IBS, and its mechanism may be related to regulating the expression of TRPV1 protein in colon of IBS visceral pain sensitive rats.
【作者单位】：山东中医药大学中医学院;
【基金】：国家重点基础研究发展计划(973计划)项目(2011CB505206) 山东省自然科学基金项目(ZR2014HQ032)
【分类号】：R245.97

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