UPLC-PDA法测定藏药翼首草不同药用部位中齐墩果酸和熊果酸的含量

发布时间：2018-06-05 08:37

  本文选题：藏药 + 翼首草　； 参考：《中国药房》2017年07期

【摘要】：目的:建立测定藏药翼首草不同药用部位中齐墩果酸和熊果酸含量的方法,并比较其不同部位之间的含量差异。方法:采用超高效液相色谱-光电二极管阵列检测器(UPLC-PDA)测定翼首草不同药用部位(全草、地上部位和地下部位)齐墩果酸和熊果酸含量。色谱柱为Acquity UPLC HSS T3(150 mm×2.1 mm,1.8μm);流动相为甲醇-0.1 mol/L乙酸铵溶液(88∶12,V/V),流速为0.2 m L/min;检测波长为210 nm;柱温为30℃;进样量为5μL。结果:齐墩果酸和熊果酸的检测质量浓度线性范围分别为10.65~1 065μg/m L(r=0.999 6)、18.8~1 880μg/m L(r=0.999 4),平均加样回收率分别为96.95%(RSD=1.24%,n=9)、98.12%(RSD=2.13%,n=9),精密度、稳定性、重复性试验的RSD均小于3%。不同药用部位的齐墩果酸和熊果酸含量的高低整体趋势为地上部位全草地下部位;翼首草全草中齐墩果酸和熊果酸平均总量为0.35%,地上部位中为0.56%,地下部位中为0.09%。结论:建立的方法快速、准确可靠、重复性好,适合于藏药翼首草不同药用部位中齐墩果酸和熊果酸含量的测定。翼首草地上部位中齐墩果酸和熊果酸含量较全草、地下部位高,建议使用地上部位入药。
[Abstract]:Objective: to establish a method for the determination of oleanolic acid and ursolic acid in different parts of Pterygium hirsutum, and to compare the content differences between different parts of oleanolic acid and ursolic acid. Methods: the contents of oleanolic acid and ursolic acid in different medicinal parts (whole grass, ground part and underground part) were determined by UPLC-PDAs by ultra-high performance liquid chromatography-photodiode array detector (UPLC-PDAs). The mobile phase consisted of methanol-0.1 mol/L ammonium acetate solution with a flow rate of 0.2ml / min, a column temperature of 30 鈩，

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