基于心肝相关理论探析HSP27和CRP对不同年龄组房颤患者的临床意义

发布时间：2018-06-05 18:48

  本文选题：心房颤动 + 热休克蛋白27　； 参考：《辽宁中医药大学》2016年硕士论文

【摘要】：目的:基于心肝相关理论探究炎性因子热休克蛋白27(HSP27)及C反应蛋白(CRP)在不同年龄组心房颤动(房颤)患者中血清的表达及其临床意义。材料与方法:从“肝”辩证,收集满足肝血亏虚、心失所养;或肝阳上亢、上扰心神;或肝郁气滞、心脉痹阻三种证型并符合西医诊断标准的老年房颤患者90例,按不同年龄段分为A组(60-69岁)30例,B组(70-79岁)30例,C组(80-89岁)30例,选取24小时动态心电图正常患者30例为正常对照组,平均年龄(67±6.8)岁。抽取房颤患者发作当天静脉血4ml,置于EDTA抗凝加抑肽酶50U试管中,并于冰盒内保存,2h内将血标本放于4℃3000rpm离心机离心10min后取血清,最后保存于-80℃低温冰箱中。采用酶联免疫法(ELISA法)分别测定CRP及HSP27浓度,比较各组间血清CRP、HSP27水平差异。结果:1.A组患者血清中HSP27的浓度为0.499±0.071mg/L,B组患者血清中HSP27的浓度为0.466±0.054mg/L,C组患者血清中HSP27的浓度为0.426±0.079mg/L,对照组血清中HSP27浓度为0.393±0.029mg/L。不同年龄组患者血清中HSP27浓度与对照组血清中HSP27浓度差异有统计学意义,各年龄组患者血清中HSP27浓度差异也均具有统计学意义,且年龄与HSP27浓度呈负相关。2.A组患者血清中CRP的浓度为0.489±0.053mg/L,B组患者血清中CRP的浓度为0.456±0.065mg/L,C组患者血清中CRP的浓度为0.427±0.036mg/L,对照组血清中CRP浓度为0.397±0.069mg/L。不同年龄组患者血清中CRP浓度与对照组血清中CRP浓度差异有统计学意义,各年龄组患者血清中CRP浓度差异也均具有统计学意义,且年龄与CRP浓度呈负相关。结论:本实验表明,房颤的发生可能与炎症有关,炎性因子HSP27及CRP水平在房颤患者中的表达较对照组更有意义,其中HSP27的表达意义尤其突出,认为可作为预测房颤发生的指标。年龄与CRP和HSP27的表达呈负性相关,说明年龄越小,CRP与HSP27对房颤风险的预测越有意义。
[Abstract]:Aim: to explore the expression and clinical significance of inflammatory factor heat shock protein 27 (HSP27) and C-reactive protein (CRP) in patients with atrial fibrillation (AF) in different age groups. Materials and methods: from the dialectical view of "liver", 90 cases of senile atrial fibrillation were collected to satisfy the deficiency of liver and blood, heart loss of nourishment, hyperactivity of liver-yang and disturbance of heart and mind, or three types of syndrome of stagnation of liver-qi and blockage of heart and veins, which met the diagnostic criteria of western medicine. According to different age groups, 30 patients were divided into group A (n = 30) and group B (n = 30). Group C (n = 30) were divided into group C (n = 30) and group C (n = 30). 30 patients with normal 24-hour ambulatory electrocardiogram were selected as normal control group, with an average age of 67 卤6.8 years. The venous blood was extracted from the patients with atrial fibrillation on the same day and placed in the EDTA anticoagulant and aprotinin 50U test tube. The blood samples were stored in the Yu Bing box for 2 hours after centrifuged 10min with 4 鈩，

本文编号：1983054