电针对动脉粥样硬化模型兔腹腔巨噬细胞中SR-AⅠ表达的影响

发布时间：2018-06-07 10:51

本文选题：动脉粥样硬化 + 电针　；参考：《辽宁中医药大学》2017年硕士论文

【摘要】：目的:观察电针“足三里”、“内关”、“关元”穴对动脉粥样硬化模型兔血脂变化的情况,腹腔巨噬细胞中SR-AI蛋白和m RNA表达情况的影响,为针刺抗动脉粥样硬化的作用机制提供新的实验依据。材料与方法:将26只健康新西兰兔适应性喂养1周后,随机选取7只为空白组喂养标准兔饲料,其余19只为造模组喂养高脂饲料。高脂喂养4周后对造模组行颈总动脉球囊损伤术,继续高脂喂养4周建立AS模型,从空白组和造模组中各随机选取1只实验兔做颈总动脉病理切片,通过HE染色观察病理形态确定造模是否成功。随后将造模组剩余18只实验兔随机分为模型组、西药组、电针组,每组6只,开始治疗。电针组选取“内关”、“足三里”、“关元”穴电针治疗;西药组给予阿托伐他汀钙片治疗。治疗4周后取材,观察颈总动脉病理形态变化,检测胆固醇(CHO)、甘油三酯(TG)、低密度脂蛋白(LDL)、高密度脂蛋白(HDL)含量,采用RT-PCR技术检测AS实验兔腹腔巨噬细胞中SR-AIm RNA的表达情况,采用Western-blot法检测SR-AI蛋白的表达情况。结果:1与空白组比较,模型组、电针组、西药组实验兔CHO、TG、LDL含量均升高,而HDL含量降低,差异有统计学意义(P0.01);与模型组比较,电针组、西药组实验兔CHO、TG、LDL含量均降低,而HDL含量增高,差异有统计学意义(P0.01);电针组与西药组组间无显著差异(P0.05)。2与空白组比较,模型组、电针组、西药组实验兔腹腔巨噬细胞中SR-AI蛋白表达水平均上调,差异具有统计学意义(P0.01);与模型组比较,电针组、西药组实验兔SR-AI蛋白的表达水平均降低,差异具有统计学意义(P0.01);电针组与西药组组间无显著差异(P0.05)。3与空白组比较,模型组、电针组、西药组实验兔腹腔巨噬细胞中SR-AIm RNA表达水平均上调,差异具有统计学意义(P0.01);与模型组比较,电针组、西药组实验兔SR-AIm RNA的表达水平均降低,差异具有统计学意义(P0.01)电针组与西药组组间无显著差异(P0.05)。结论:1电针内关、关元、足三里穴可改善动脉粥样硬化模型兔动脉粥样斑块以及调节血脂紊乱情况。2电针内关、关元、足三里穴可抑制动脉粥样硬化模型兔腹腔巨噬细胞中SR-AI蛋白和m RNA的表达,这可能是针刺防治AS的又一机制。
[Abstract]:Objective: to observe the effect of electroacupuncture at "Zusanli", "Neiguan" and "Guanyuan" on the changes of blood lipid and the expression of SR-AI protein and m RNA in peritoneal macrophages of atherosclerotic rabbits. To provide a new experimental basis for the mechanism of acupuncture anti-atherosclerosis. Materials and methods: after 26 healthy New Zealand rabbits were fed adaptively for one week, 7 rabbits were randomly selected as the blank group to feed the standard rabbit feed, and the remaining 19 rabbits were fed the high-fat diet for the model group. After 4 weeks of high fat feeding, the common carotid artery injury was performed in the model group, and the model of high fat feeding for 4 Zhou Jianli was continued. One rabbit was randomly selected from the blank group and the model group to make pathological sections of the common carotid artery. The pathological morphology was observed by HE staining to determine whether the model was successful or not. Then the remaining 18 rabbits in the model group were randomly divided into three groups: model group, western medicine group, electroacupuncture group, 6 rabbits in each group. The electroacupuncture group was treated with "Neiguan", "Zusanli" and "Guanyuan", and the western medicine group was treated with Atto vastatin calcium tablets. After 4 weeks of treatment, the pathological changes of the common carotid artery were observed, and the contents of cholesterol, triglyceride, low density lipoprotein (LDL) and high density lipoprotein (HDL) were detected. The expression of SR-AIm RNA in peritoneal macrophages of as experimental rabbits was detected by RT-PCR technique. The expression of SR-AI protein was detected by Western-blot method. Results compared with the control group, the content of HDL in the model group, electroacupuncture group and western medicine group all increased, but the content of HDL decreased, the difference was statistically significant (P0.01), compared with the model group, the content of HDL in the electroacupuncture group and the western medicine group were all lower than that in the model group. There was no significant difference between the electroacupuncture group and the western medicine group. Compared with the blank group, the expression of SR-AI protein in the peritoneal macrophages of model group, electroacupuncture group and western medicine group were all up-regulated. Compared with the model group, the expression of SR-AI protein in the electroacupuncture group and the western medicine group were all decreased, the difference was statistically significant (P 0.01), there was no significant difference between the electroacupuncture group and the western medicine group, and there was no significant difference between the electroacupuncture group and the control group, the model group. The expression of SR-AIm RNA in peritoneal macrophages of experimental rabbits in electroacupuncture group and western medicine group were all up-regulated, the difference was statistically significant (P 0.01), compared with model group, the expression level of SR-AIm RNA in electroacupuncture group and western medicine group were all lower than that in model group. There was no significant difference between electroacupuncture group and western medicine group. Conclusion the electroacupuncture of Neiguan, Guan Yuan and Zusanli can improve the atherosclerotic plaque and regulate the disorder of blood lipids in rabbit model of atherosclerosis. 2 electroacupuncture at Neiguan, Guanyuan, and Guanyuan can improve the atherosclerotic plaque in rabbits. Zusanli point can inhibit the expression of SR-AI protein and m RNA in peritoneal macrophages of atherosclerotic rabbits, which may be another mechanism of acupuncture to prevent and treat as.
【学位授予单位】：辽宁中医药大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R245

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