三法三穴干预坐骨神经损伤大鼠运动功能刺激参数研究

发布时间：2018-06-12 08:58

本文选题：推拿 + 参数　；参考：《北京中医药大学》2017年硕士论文

【摘要】：[目的]通过正交试验指导下比较行为学、腓肠肌功能、免疫组织化学相关指标的改变,从行为学下研究推拿手法参数中的力量、时间、频率对周围神经损伤大鼠运动功能恢复的影响。探究推拿促进坐骨神经损伤大鼠运动功能恢复的最佳刺激参数组合。[方法]以坐骨神经损伤为例研究周围神经损伤,以SD大鼠为实验动物,采用坐骨神经加持损伤模型。7天后使用"推拿手法模拟仪"模拟三法(拨法、揉法、点法)推拿手法在三个穴道(殷门、承山、阳陵泉)上进行干预。以力量(A)、时间(B)、频率(C)三种因素,每个因数各有三组不同水平刺激,力量为2N、4N、6N;时间1min、3min、5min;频率30次/min、60次/min、100次/min。用三因素三水平的正交试验设计方案筛选推拿干预促进SNI大鼠运动功能恢复的最佳刺激参数组合。1.从行为学角度,测定斜板实验及坐骨神经功能指数以评价大鼠肌力功能恢复情况。2.从功能角度,测定腓肠肌湿重恢复率评价推拿对肌肉萎缩程度的改善。3.采用免疫组织化学法测定脊髓腹角内NGF的表达情况,探寻推拿促进NGF表达的最佳刺激参数。[结果]1.斜板实验造模7天后斜板实验数据分析,正常组、假手术组、模型组之间存在统计学差异(P0.01)。推拿干预20次后,推拿组(九组)之间采用正交试验的直观分析法,研究结果显示三因素的主次关系为:时间力量频率。最佳刺激参数组合是力量4N,时间1min,频率60次/min。推拿组(九组)之间采用方差分析法得出,三因素力量(A)、时间(B)、频率(C)对结果的影响无统计学差异(P0.05)。但从F值的大小判断(正交试验中对应因素的F值越大,该因素对结果的影响也越大),来看,可认为各因素的主次关系为:时间力量频率,而以短时间治疗效果为佳。这与直观分析法得出的结果一致。2.坐骨神经功能指数推拿干预20次后,推拿组(九组)之间采用正交试验的直观分析法,研究结果显示三因素的主次关系为:频率时间力量。最佳刺激参数组合是力量2N,时间1min,频率30次/mmin。推拿组(九组)之间采用方差分析法得出,三因素力量(A)、时间(B)、频率(C)对结果的影响无统计学差异(P0.05)。但从F值的大小判断(对应因素的F值越大,该因素对结果的影响也越大),来看,可认为各因素的主次关系为:频率时间力量,这与直观分析法得出的结果一致。3.腓肠肌湿重恢复率造模7天后,推拿组与模型组腓肠肌湿重恢复率无显著性差异(P0.05),推拿干预20次后,推拿组腓肠肌湿重恢复率测定结果与模型组比较有显著性差异(P0.05),代表腓肠肌萎缩后进行推拿干预可有预防肌肉萎缩的效果。推拿干预20次后,推拿组(九组)之间采用正交试验的直观分析法,研究结果显示三因素的主次关系为力量＞频率时间,根据F值的大小,最佳刺激参数组合是力量4N,时间5mmin,频率30次/min。推拿组(九组)之间采用方差分析法得出,三因素力量(A)、时间(B)、频率(C)的主次关系无显著性差异(P0.05)。但从F值的大小判断(对应因素的F值越大,该因素对结果的影响也越大),来看,可认为各因素的主次关系为:力量频率时间,这与直观分析法得出的结果一致。4.脊髓腹角NGF光密度推拿干预20次后,推拿组(九组)之间采用正交试验的直观分析法,研究结果显示三因素的主次关系为:力量时间频率。最佳刺激参数组合是力量4N,时间1min,频率60次/min。推拿组(九组)之间采用方差分析法得出,三因素力量(A)、时间(B)、频率(C)对结果的影响有统计学差异(P0.05)。各因素的主次关系为力量时间频率,但从单因素来看,A(力量)因素、B(时间)因素的影响显著(P0.05),C(频率)因素的影响不显著(P0.05)。[结论]1.通过三法三穴干预可以促进SNI大鼠运动功能的恢复,刺激参数为中等力量、短时间、中等频率为佳。而手法力量过重、时间过长时疗效反而降低。2.通过三法三穴干预可以调控SNI大鼠脊髓腹角NGF的表达。通过NGF的提高可使运动功能恢复而刺激参数以中等力量、短时间、中频率为佳。
[Abstract]:[Objective] to compare the effect of behavior, gastrocnemius function and immuno histochemical related indexes under the guidance of orthogonal test, and study the influence of the strength, time and frequency of the manipulative parameters on the recovery of motor function in rats with peripheral nerve injury. With the sciatic nerve injury as an example, the peripheral nerve injury was studied with the sciatic nerve injury as an example. SD rats were used as experimental animals. The three methods (dial method, kneading method, point method) were used to intervene in the three Cave (Yin gate, Chengshan and Yang Ling spring) using the sciatic nerve injury model after.7 days. ), three factors of frequency (C), each factor has three groups of different levels of stimulation, power is 2N, 4N, 6N, time 1min, 3min, 5min, frequency 30 /min, 60 /min, 100 /min. with three factors three level orthogonal design scheme screening the best stimulus parameter combination to promote the function recovery of SNI rats. The oblique plate experiment and the sciatic nerve function index to evaluate the recovery of muscle function in rats.2. from the functional point of view, the evaluation of the recovery rate of the gastrocnemius wet weight and the improvement of the degree of muscular atrophy by the massage.3., the expression of NGF in the ventral horn of the spinal cord was measured by immunohistochemistry, and the best stimulation parameters to promote the expression of NGF were explored. [result]1. There was a statistical difference between the normal group, the sham operation group and the model group (P0.01). After 20 times of the massage intervention, the orthogonal test was used by the massage group (nine groups). The results showed that the main and secondary relation of the three factors was the time force frequency. The best combination of the stimulus parameters was the strength 4N, Time 1min, frequency 60 times /min. massage group (nine groups) between the use of variance analysis method, three factor power (A), time (B), frequency (C) on the effect of no statistical difference (P0.05). But from the size of the F value (the greater the F value of the corresponding factors in the orthogonal test, the greater the impact of the factor on the results), it is considered that the main and secondary factors of the factors can be considered. The relationship is: time power frequency, and the effect of short time treatment is better. This is consistent with the results obtained by the intuitionistic analysis method. After 20 times of.2. sciatic nerve function index massage intervention, the intuitionistic analysis of the orthogonal test between the massage group (nine groups) is adopted, and the results show that the main and secondary relations of the three factors are the frequency time force and the best stimulus parameter group. The combination is power 2N, time 1min, frequency 30 times /mmin. massage group (nine groups) using variance analysis method, three factor power (A), time (B), frequency (C) has no statistical difference (P0.05). But judging from the size of F value (the greater the F value of the corresponding factor, the greater the effect of the factor on the result), it can be considered the main factor. The secondary relationship was: frequency time force, which was consistent with the results obtained by the visual analysis method, the recovery rate of gastrocnemius wet weight recovery rate of.3. was no significant difference (P0.05) after 7 days. After 20 times of massage, the determination of the recovery rate of the gastrocnemius wet weight in the massage group was significantly different from the model group (P0.05), and the generation of the gastrocnemius muscle weight recovery rate was significantly different from the model group. The effect of massage after gastrocnemius atrophy could prevent muscle atrophy. After 20 times of massage, a visual analysis of orthogonal test was used between the nine groups of massage group (Group). The results showed that the main and secondary relationship of the three factors was power > frequency time. According to the size of F, the best combination of stimulus parameters was power 4N, time 5mmin, frequency 30. The second /min. massage group (nine groups) by variance analysis method, three factor power (A), time (B), frequency (C) of the primary and secondary relations of no significant difference (P0.05). But from the size of the F value (the value of the corresponding factor, the greater the value of the factor, the greater the impact of the factor on the results), it is considered that the main and secondary relations of the factors are: power frequency time, this is the same. The results of the intuitionistic analysis were consistent with the.4. NGF light density massage for 20 times, and the orthogonal test was used for the intuitional analysis between the massage group (nine groups). The results showed that the main and secondary relations of the three factors were power time frequency. The best combination of stimulus parameters was power 4N, time 1min, and frequency 60 /min. massage groups (nine groups). The results of variance analysis showed that three factors (A), time (B) and frequency (C) had statistical differences (P0.05). The main and secondary relationships of all factors were power time frequency, but from the single factor, the influence of A (strength), B (time) factors was obvious (P0.05) and C (frequency) factors were not significant (P0.05). [conclusion]1. through three methods and three points. Intervention can promote the recovery of motor function of SNI rats. The stimulation parameters are medium strength, short time and moderate frequency. While the manipulative force is too heavy and the time is too long, the curative effect reduces the expression of NGF in the ventral horn of the spinal cord of SNI rats through the intervention of three methods and three points. Through the improvement of NGF, the exercise function can be recovered and stimulate parameters in the middle of the rat. Power, short time, and good frequency.
【学位授予单位】：北京中医药大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R245

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