针刺对哮喘模型大鼠肺组织中TGF-β1表达的调控

发布时间：2018-06-13 21:51

本文选题：支气管哮喘 + 针刺　；参考：《中国针灸》2017年07期

【摘要】：目的:探讨针刺对支气管哮喘大鼠肺组织中转化生长因子β1(transforming growth factorβ1,TGF-β1)表达的调控。方法:将40只SPF级SD大鼠随机分为空白组、模型组、针刺组、浅刺不留针组,每组10只。模型组、针刺组、浅刺不留针组制作SD大鼠哮喘模型。空白组、模型组不予干预;针刺组自造模之日起于每次雾化激发前先进行针刺操作,穴取"大椎""肺俞""风门",每次留针20 min;浅刺不留针组自造模之日起于每次雾化激发前针刺,针刺时只刺入皮下、不留针,取穴同针刺组,均隔日针刺1次,共治疗7次。采用苏木素伊红(HE)染色法观察大鼠肺组织的病理变化,免疫组织化学染色法和免疫荧光染色法检测肺组织中TGF-β1蛋白的表达,ELISA检测大鼠支气管肺泡灌洗液(bronchoalveolar lavage fluid,BALF)及血清中TGF-β1表达。结果:模型组大鼠肺组织支气管管腔中有黏液栓形成,支气管上皮结构不规则,气道平滑肌增厚,局部出现断裂,管腔狭窄,有大量炎性细胞浸润;针刺组较模型组有明显改善;浅刺不留针组无明显改善。模型组TGF-β1阳性表达IOD值较空白组明显增加(P0.05);针刺组阳性表达低于模型组和浅刺不留针组(均P0.05)。针刺组BALF及血清中TGF-β1含量均低于模型组、浅刺不留针组(均P0.05)。结论:针刺可能通过抑制气道TGF-β1的表达,减轻哮喘气道炎性反应及重塑的发生。
[Abstract]:Aim: to investigate the effect of acupuncture on the expression of transforming growth factor 尾 1(transforming growth factor 尾 1 (TGF- 尾 1) in lung tissues of asthmatic rats. Methods: forty SPF SD rats were randomly divided into blank group, model group, acupuncture group and non-acupuncture group with 10 rats in each group. Model group, acupuncture group and superficial needling group were used to make asthma model of SD rats. Blank group, model group, no intervention; acupuncture group since the day of making the model before each atomization excitation, acupuncture operation, Acupoint "Dazhui", "Feshu" and "Fengmen" were used for 20 mins each time, and the group without superficial needling was treated 7 times every other day. The pathological changes of rat lung tissue were observed by hematoxylin eosin (HEH) staining. The expression of TGF- 尾 1 protein in lung tissue was detected by immunohistochemical staining and immunofluorescence staining. The expression of TGF- 尾 1 in bronchoalveolar lavage fluididine (BALF1) and serum in rat bronchoalveolar lavage fluid (bronchoalveolar lavage) and serum were detected by Elisa. Results: in the model group, mucus embolus was formed in the bronchial lumen of lung tissue, the bronchial epithelial structure was irregular, airway smooth muscle thickened, local rupture appeared, the lumen was narrow and a large number of inflammatory cells infiltrated. The acupuncture group was better than the model group, but the superficial needling group had no obvious improvement. The positive expression of TGF- 尾 1 in the model group was significantly higher than that in the control group (P 0.05), and the positive expression of TGF- 尾 1 in the acupuncture group was lower than that in the model group and the superficial needling group (all P 0.05). The levels of BALF and TGF- 尾 _ 1 in serum of acupuncture group were lower than those of model group, while those of superficial needling group were not (P 0.05). Conclusion: acupuncture may reduce airway inflammatory response and remodeling by inhibiting the expression of TGF- 尾 1 in airway.
【作者单位】：华北理工大学中医学院;
【基金】：国家自然科学基金项目:81303046
【分类号】：R245;R-332

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