针砂马准灸对兔膝骨关节炎软骨组织中JNK信号通路表达的影响

发布时间：2018-06-13 23:32

本文选题：回医 + 针砂马准灸　；参考：《辽宁中医杂志》2017年06期

【摘要】：目的:观察回医针砂马准灸对兔膝骨关节炎(KOA)模型软骨细胞c-Jun氨基末端激酶(JNK)信号通路中JNK1、2的表达,探讨针砂马准灸治疗膝骨性关节炎的作用机理。方法:新西兰大耳兔40只,随机分为针砂马准灸组、艾灸组、模型组和空白对照组,参照右后肢膝关节伸直位石膏固定法制备兔KOA模型。造模完成后,分别给予针砂马准灸治疗、艾灸治疗,模型组、空白组不予任何处理。治疗结束后取兔膝软骨组织。采用蛋白印迹法检测JNK1、JNK2蛋白表达。结果:相比空白组,模型组JNK1、JNK2表达均升高(P0.01),差异有统计学意义;与模型组比较,针砂马准灸组、艾灸组JNK1、JNK2表达均降低(P0.05),差异有统计学意义,针砂马准灸组与艾灸组间差异无统计学意义(P0.05)。结论:针砂马准灸治疗兔KOA可能与激活JNK信号通路,引起JNK1、JNK2表达的下降有关。
[Abstract]:Objective: to observe the expression of JNK1t2 in the chondrocytes of rabbit knee osteoarthritis model and to explore the mechanism of acupuncture moxibustion in the treatment of knee osteoarthritis. Methods: forty New Zealand rabbits were randomly divided into three groups: moxibustion group, model group and blank control group. After the model was made, acupuncture-sand moxibustion treatment, moxibustion therapy, model group and blank group were given without any treatment. The cartilage tissue of rabbit knee was removed after treatment. The expression of JNK1 and JNK2 protein was detected by Western blot. Results: compared with the blank group, the expression of JNK1 / JNK2 in the model group was significantly higher than that in the control group, and the expression of JNK1 / JNK2 in the moxibustion group and moxibustion group was significantly lower than that in the model group. There was no significant difference between moxibustion group and moxibustion group (P 0.05). Conclusion: the treatment of KOA with needle-sand moxibustion may be related to the activation of JNK signal pathway and the decrease of JNK1 / JNK2 expression.
【作者单位】：宁夏医科大学总医院中医骨伤科;宁夏医科大学回医药现代化省部共建教育部重点实验室;宁夏医科大学总医院胸科医院中医康复科;
【基金】：国家自然科学基金(81560814)
【分类号】：R245

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