糖胰生1号方对2型糖尿病的干预及对大鼠GLP-1影响的实验研究

发布时间：2018-06-14 07:02

本文选题：糖胰生1号方 + 二甲双胍　；参考：《山东中医药大学》2016年硕士论文

【摘要】：目的:探讨以清热解毒、健脾补肾、宣肺调肠、畅达三焦为组方原则的糖胰生1号方对中重度血糖升高的2型糖尿病的临床疗效,探究其原理。方法:(1)临床研究:从符合条件的患者中选取70例,随机抽35例作为试验组,35例对照。两组均在基础治疗的基础上,再分别给予糖胰生1号方联合二甲双胍(格华止)和二甲双胍(格华止)治疗,以12周为一个疗程。比较研究前后,患者的临床症状、体征、实验指标的差异。(2)实验研究:采用STZ作为造成2型糖尿病大鼠的实验用药。从造模成功的Wistar雄性大鼠中选取4组,每组10只,共40只:模型组、二甲双胍组、糖胰生1号方低剂量组、糖胰生1号方高剂量组。空白组为10只正常大鼠。给药观察6周后,分别采用并用酶联免疫方法检测门静脉中GLP-1、免疫组化法测定小肠与结肠中GLP-1的表达。结果:(1)临床研究:糖胰生1号方不仅对2型糖尿病患者的临床症状及体征具有明显的改善,在降低血糖、HbA1c、增加C肽及胰岛素释放及调节血脂的方面临床亦有明显疗效,优于对照组。(2)实验研究:糖胰生1号方不仅可明显改善糖尿病大鼠的空腹血糖、减轻体重、促进引导素分泌,还可明显增强GLP-1的分泌与表达,高剂量中药组对大鼠的疗效优于各对照组。结论:以清热解毒、健脾补肾、宣肺调肠、畅达三焦为组方原则的糖胰生1号方对中重度血糖升高的2型糖尿病患者疗效确切,值得深入研究,推测可能与改善糖代谢、激发胰岛功能有关。
[Abstract]:Objective: to explore the clinical effect of Tangyi Shengsheng 1 prescription on type 2 diabetes mellitus with moderate and severe hyperglycemia based on the principle of clearing heat and detoxification, invigorating spleen and kidney, promoting lung and regulating intestine, and Changda Sanjiao. Methods Clinical study: 70 patients were selected from eligible patients and 35 patients were randomly selected as control group. On the basis of basic treatment, the two groups were treated with Tang-Yisheng No. 1 prescription combined with metformin and metformin for 12 weeks. To compare the clinical symptoms, signs and experimental indexes of the patients before and after the study. Experimental study: STZ was used as the experimental drug to cause type 2 diabetes in rats. A total of 40 male Wistar rats were divided into 4 groups: model group, metformin group, low dose group and high dose group. The blank group consisted of 10 normal rats. After 6 weeks of administration GLP-1 was detected by enzyme-linked immunosorbent assay (Elisa) and the expression of GLP-1 in small intestine and colon was detected by immunohistochemistry. Results: the clinical study showed that Tang Yisheng No. 1 prescription not only improved the clinical symptoms and signs of type 2 diabetes mellitus, but also decreased blood glucose and HbA1c, increased the release of C peptide and insulin, and regulated blood lipids. The experimental study showed that Tang Yisheng No. 1 could not only improve the fasting blood glucose, reduce body weight, promote the secretion of guiding hormone, but also enhance the secretion and expression of GLP-1 in diabetic rats. The therapeutic effect of high dose Chinese medicine group on rats was better than that of other control groups. Conclusion: Tang Yisheng 1 prescription, which is based on the principle of clearing heat and detoxifying toxin, invigorating spleen and kidney, promoting lung and regulating intestine, and Changda Sanjiao, is effective in treating type 2 diabetes with moderate and severe hyperglycemia. It is worthy of further study, and it may be speculated and improved on glucose metabolism. Activation of islet function.
【学位授予单位】：山东中医药大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：R259

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