不同时机艾灸对寒湿凝滞型痛经大鼠子宫组织微血管密度及血管活性物质含量的影响

发布时间：2018-06-14 23:23

本文选题：原发性痛经 + 寒湿凝滞型　；参考：《河北医科大学》2017年硕士论文

【摘要】：目的:观察不同时机艾灸干预对寒湿凝滞型原发性痛经(Primary Dysmenorrhea,PD)大鼠行为学、子宫组织微血管密度(MVD)、子宫组织前列腺素F2α(PGF2α)、前列腺素E2(PGE2)、加压素(AVP)、一氧化氮(NO)、内皮素(ET-1)含量以及血浆血栓素B2(TXB2)、6-酮-前列腺素F1α(6-keto-PGF1α)含量的影响,以探讨其对寒湿凝滞型原发性痛经模型大鼠止痛效应影响的差异性及其可能的作用机制。以期为临床选择最佳时机的艾灸介入治疗寒湿凝滞型原发性痛经提供可靠的实验依据。方法:将60只Wistar雌性大鼠适应性喂养7 d后,采用阴道涂片法,筛选出处于动情间期大鼠45只,并将其随机分为对照组(Control)、模型组(Model)、预先灸组(Pre-Mox)、即刻灸组(Im-Mox)和预先即刻灸组(PI-Mox)(每组9只)。对照组于大鼠股部给予皮下注射生理盐水,1次/d,连续10 d(第1 d、10 d注射0.5 m L/只,第2~9 d 0.2 m L/只)。其余4组大鼠均采用(0±1)℃冰水浴结合苯甲酸雌二醇注射法制备寒湿凝滞型痛经模型,将大鼠后肢及下腹部浸入(0±1)℃冰水混合物中(室温23±2℃)进行寒冷刺激,1次/d,20 min/次,同时给予大鼠股部皮下注射苯甲酸雌二醇,1次/d,连续10 d(第1 d、10 d注射0.5 mg/只,第2~9 d 0.2 mg/只)。预先灸组于造模期间第8 d开始治疗,第10 d结束治疗,共3 d;即刻灸组于造模第11 d注射完缩宫素后(即痛经模型复制成功后)开始治疗,共1 d;预先即刻灸组于造模期间第8 d开始治疗,第11 d结束治疗,共4 d。3个艾灸组均采用直径为7 mm粗细的艾条,于神阙、关元穴处同时施温和灸,10 min/穴,1次/d。对照组、模型组均采用与艾灸组相同的体位固定,10 min/次,1次/d。除对照组外,其余4组寒湿凝滞型PD模型大鼠模型均注射缩宫素后观察其行为学反应,待行为学检测结束后,将大鼠断头取血后,于冰盘上迅速分离出“Y”字形子宫左侧子宫组织,以冷藏的生理盐水洗净拭干称重,加入生理盐水碾磨,制成30%的子宫组织匀浆,于低温4℃时3000 rpm离心15 min,取上清液,用放射免疫分析法检测大鼠子宫组织PGE2、AVP、ET-1含量,酶联免疫法检测大鼠子宫组织PGF2α含量,生物化学技术检测大鼠子宫组织NO含量。右侧子宫组织用浓度为4%的多聚甲醛溶液进行固定,石蜡包埋、切片,一部分采用免疫组化法检测子宫组织MVD,剩余部分用于HE染色,于光镜下观察各组大鼠子宫组织MVD和子宫组织细胞的形态学改变。结果:1不同时机艾灸对寒湿凝滞型PD大鼠行为学的影响扭体行为学观察结果显示:艾灸干预后,预先灸组、即刻灸组、预先即刻灸组痛经大鼠的潜伏期均明显延长、扭体次数减少、扭体总分降低,与模型组比较,差异均有统计学意义(P0.01);3个艾灸组相比,即刻灸组、预先即刻灸组较预先灸组痛经大鼠的扭体次数及扭体总分均明显减少和降低(P0.01),预先即刻灸组较预先灸组痛经大鼠的潜伏期明显延长(P0.01);预先即刻灸组与即刻灸组比较,预先即刻灸组较即刻灸组痛经大鼠的潜伏期明显延长、扭体次数及扭体总分均明显减少和降低(P0.05或P0.01)。2不同时机艾灸对寒湿凝滞型PD大鼠子宫组织细胞病理形态学的影响子宫组织HE染色后,置于光镜下,一般形态学观察显示:对照组大鼠子宫组织形态规则,无水肿,大量中性粒细胞;模型组大鼠子宫组织形态较规则,组织严重水肿,充血,大量中性粒细胞和淋巴细胞;预先灸组、即刻灸组、预先即刻灸组大鼠子宫组织均形态规则,组织轻度水肿,无充血,大量中性粒细胞,少量淋巴细胞。3不同时机艾灸对寒湿凝滞型PD大鼠子宫组织MVD值的影响模型组大鼠子宫组织MVD明显减少,与对照组比较,差异有统计学意义(P0.05);预先灸组、即刻灸组、预先即刻灸组与模型组比较,3个艾灸组痛经大鼠子宫组织MVD均明显增多,差异有统计学意义(P0.01);预先即刻灸组与预先灸组比较,预先即刻灸组较预先灸组MVD明显增多(P0.05);即刻灸组与预先灸组比较,即刻灸组较预先灸组MVD虽有数值差异,但无统计学差异(P0.05)。预先即刻灸组与即刻灸组比较,预先即刻灸组较即刻灸组虽有数值差异,但无统计学差异(P0.05)。4不同时机艾灸对寒湿凝滞型PD大鼠血浆TXB2和6-keto-PGF1α含量的影响模型组痛经大鼠血浆TXB2含量及TXB2/6-keto-PGF1α比值均明显升高、6-keto-PGF1α含量明显降低,与对照组相比,差异均有统计学意义(P0.01);艾灸干预后,预先灸组、即刻灸组、预先即刻灸组痛经大鼠血浆TXB2含量及TXB2/6-keto-PGF1α比值均明显降低、6-keto-PGF1α含量明显升高,与模型组相比,差异均有统计学意义(P0.01);预先即刻灸组与预先灸组比较,预先即刻灸组较预先灸组痛经大鼠血浆TXB2含量及TXB2/6-keto-PGF1α比值均明显降低,6-keto-PGF1α含量明显升高(P0.01或P0.05);即刻灸组与预先灸组比较,即刻灸组较预先灸组血浆TXB2、6-keto-PGF1α含量及TXB2/6-keto-PGF1α比值虽有数值差异,但无统计学意义(P0.05);预先即刻灸组与即刻灸组比较,预先即刻灸组较即刻灸组痛经大鼠血浆TXB2含量及TXB2/6-keto-PGF1α比值均明显降低、6-keto-PGF1α含量明显升高(P0.01)。5不同时机艾灸对寒湿凝滞型PD大鼠子宫组织PGF2α、PGE2含量及PGF2α/PGE2比值的影响模型组痛经大鼠子宫组织PGF2α的含量及PGF2α/PGE2比值均明显升高,PGE2的含量明显降低,与对照组比较,差异均有统计学意义(P0.01)。艾灸干预后,预先灸组、即刻灸组、预先即刻灸组痛经大鼠子宫组织PGF2α的含量及PGF2α/PGE2比值均明显降低、PGE2含量明显升高,与模型组相比,差异均有统计学意义(P0.01或P0.05)。预先即刻灸组与预先灸组比较,预先即刻灸组较预先灸组子宫组织PGF2α的含量及PGF2α/PGE2比值均明显降低(P0.01),PGE2的含量明显升高(P0.01)。即刻灸组与预先灸组比较,即刻灸组较预先灸组PGF2α的含量及PGF2α/PGE2比值均明显降低(P0.05),PGE2含量明显升高(P0.01)。预先即刻灸组与即刻灸组比较,预先即刻灸组较刻灸组痛经大鼠子宫组织PGF2α的含量及PGF2α/PGE2比值均明显降低(P0.01),PGE2的含量明显升高(P0.01)。6不同时机艾灸对寒湿凝滞型PD大鼠子宫组织AVP含量的影响模型组痛经大鼠子宫组织AVP的含量明显升高,与对照组比较,差异有统计学意义(P0.01)。艾灸干预后,预先灸组、即刻灸组、预先即刻灸组3个艾灸组痛经大鼠子宫组织AVP含量均明显降低,与模型组比较,差异有统计学意义(P0.05或P0.01)。预先即刻灸组、即刻灸组、预先灸组3组两两比较,3组子宫组织AVP含量虽各有数值差异,但无统计学意义(P0.05)。7不同时机艾灸对寒湿凝滞型PD大鼠子宫组织ET-1、NO含量的影响模型组痛经大鼠子宫组织ET-1的含量明显升高,NO的含量明显降低,与对照组比较,差异均有统计学意义(P0.01)。艾灸干预后,预先灸组、即刻灸组、预先即刻灸组痛经大鼠子宫组织ET-1的含量明显降低、NO含量明显升高,与模型组相比,差异均有统计学意义(P0.01)。预先即刻灸组与预先灸组比较,预先即刻灸组较预先灸组子宫组织ET-1的含量明显降低、NO的含量明显升高(P0.01)。即刻灸组与预先灸组比较,即刻灸组较预先灸组ET-1的含量明显降低、NO含量明显升高(P0.05)。预先即刻灸组与即刻灸组比较,预先即刻灸组较刻灸组痛经大鼠子宫组织ET-1的含量明显降低、NO的含量明显升高(P0.05)。结论:1预先灸、即刻灸及预先即刻灸的不同时机介入均可明显减轻寒湿凝滞型PD大鼠腹部收缩及扭体反应,明显延长其潜伏期,但其效果尤以预先即刻灸最为明显,即刻灸次之。说明不同时机艾灸对寒湿凝滞型PD模型大鼠均有良好的镇痛效果,但预先即刻灸的效果更加显著。2三种不同时机艾灸均可改善寒湿凝滞型PD模型大鼠子宫组织充血、水肿状态。但预先灸、即刻灸及预先即刻灸3组之间对大鼠子宫组织病理形态学改变无明显差异。3三种不同时机艾灸均可增加寒湿凝滞型PD模型大鼠子宫组织MVD,有效改善子宫局部微循环。但其中尤以预先即刻灸组效果最佳,明显优于预先灸和即刻灸。其作用可能是通过艾火的温通、经络的传热及神经的传递对子宫产生的热效应,以增加血流量,改变局部血液循环,进而达到缓解疼痛之目的。64三种不同时机艾灸均可降低寒湿凝滞型PD模型大鼠血浆TXB2含量及TXB2/6-keto-PGF1α比值;升高6-keto-PGF1α的含量,其中尤以预先即刻灸最为明显。说明不同时机艾灸对上述各项指标水平均有良好的调节作用,但预先即刻灸的调节作用更加显著。其作用机制可能是通过调节血小板活性和血管张力,改善血管收缩状态,使子宫局部血流量增加,而达到止痛之目的。5三种不同时机艾灸均可降低寒湿凝滞型PD模型大鼠子宫组织PGF2α含量及PGF2α/PGE2比值;提高PGE2的水平,但其作用尤以预先即刻灸最为明显,即刻灸次之。说明不同时机艾灸对PGF2α和PGE2的异常水平均有良好的调节作用,且预先即刻灸的调节作用更加显著,其作用机制可能是通过恢复PGF2α和PGE2的平衡,改善机体内分泌环境,进而抑制子宫平滑肌及血管痉挛性收缩,改善局部的缺血缺氧状态,从而起到镇痛作用。6三种不同时机艾灸均可明显降低寒湿凝滞型PD模型大鼠子宫组织AVP含量,且三者之间无明显差异。其作用机制可能是通过降低AVP含量,进一步减少PG合成与释放,从而有效抑制子宫平滑肌痉挛性收缩,改善局部血液循环,增加血流量,缓解缺血状态,从而发挥其镇痛之效应。7三种不同时机艾灸均可降低ET-1含量、升高NO含量,其中预先即刻灸最为明显,即刻灸次之。说明不同时机艾灸均可调节子宫组织ET-1和NO的失衡状态,且预先即刻灸的调节作用更加显著。其作用机制可能是通过舒张子宫血管平滑肌,抑制其收缩,改善子宫组织微循环,增加其血流量,以达到缓解疼痛之目的。
[Abstract]:Objective: To observe the behavior of Primary Dysmenorrhea (PD), uterine tissue microvascular density (MVD), prostaglandin F2 alpha (PGF2 a), prostaglandin E2 (PGE2), vasopressin (AVP), nitrogen monoxide (NO), endothelin (ET-1), plasma thromboxane (thromboxane), and plasma thromboxane (PD). The effect of the content of adenine F1 alpha (6-keto-PGF1 alpha) on the difference and possible mechanism of its effect on the analgesic effect of the cold wet and stagnant primary dysmenorrhea model rats is discussed in order to provide the experimental basis for the best time to choose the best time to treat the cold wet and stagnant primary dysmenorrhea by the moxibustion intervention. Method: 60 females of Wistar are large. After 7 d of adaptive feeding, 45 rats were selected by vaginal smear and divided into control group (Control), model group (Model), pre moxibustion group (Pre-Mox), immediate moxibustion group (Im-Mox) and pre moxibustion group (PI-Mox) (9 in each group). The control group was given subcutaneous injection of saline, 1 times /d, and 10 d in the control group. (first D, 10 d injection 0.5 m L/, 2~9 D 0.2 m L/). The remaining 4 groups of rats were used (0 + 1) centigrade ice water bath combined with estradiol benzoate to prepare the cold wet and stagnant dysmenorrhea model. The rats' hind limbs and lower abdomen were immersed in (0 + 1) C ice water mixture (room temperature 23 + 2 c) for cold stimulation, 1 times /d, 20 min/ times, and rat share at the same time. The subcutaneous injection of estradiol benzoate, 1 /d, continuous 10 d (first D, 10 d injection 0.5 mg/, 2~9 D 0.2 mg/). The pre moxibustion group was treated with eighth d during the mould making, tenth D ended with a total of 3 D; the immediate moxibustion group was treated with eleventh d after injection of the uterus (after the success of the dysmenorrhea model replication), a total of the moxibustion group in advance was made in the moxibustion group in the model of advance moxibustion group in the model of advance moxibustion group in the model of advance moxibustion group in model making in advance moxibustion group in model in advance moxibustion group in model During the period of eighth D treatment and eleventh D for the end of the treatment, a total of 4 D.3 moxibustion groups were all with a diameter of 7 mm thickness, at the Shen que, Guan yuan point at the same time temperature and moxibustion, 10 min/ points, 1 /d. control group, the model group adopted the same body position with the moxibustion group, 10 min/ times, 1 /d. except the control group, the other 4 groups of cold wet stagnation model PD model rats. After the model was injected with oxytocin, the behavioral response was observed. After the end of the behavior test, the left uterus tissue of the "Y" shaped uterus was quickly separated on the ice plate after the end of the behavior test. 30% of the uterine tissue homogenate was made by adding physiological saline and milling with normal saline. At 4 centigrade of 3000 rpm centrifugation at low temperature. 15 min, taking the supernatant, using radioimmunoassay to detect the content of PGE2, AVP, ET-1 in the uterus tissue of rats. The content of PGF2 alpha in the uterus tissue of rats was detected by enzyme linked immunoassay. The content of NO in the uterus tissue of rats was detected by biochemical technique. The right uterus tissue was fixed with a concentration of 4% Formaldehyde Solution, paraffin embedding, section, and part of the immunization. The histochemical method was used to detect the MVD of the uterus tissue, the remaining part was used for the HE staining. The morphological changes of the MVD and the uterine tissue in the uterus were observed under the light microscope. Results: 1 the effects of moxibustion on the behavior of the cold wet and stagnant PD rats were observed: the prognosis of the moxibustion and the moxibustion group, the immediate moxibustion group, beforehand, The latent period of dysmenorrhea in the group of moxibustion was obviously prolonged, the number of twisting body decreased and the total score of torsional body decreased. The difference was statistically significant (P0.01) compared with the model group. Compared with the 3 moxibustion groups, the number of twisting bodies and the total twisting body score of the group of immediate moxibustion group and the pre moxibustion group in advance were significantly reduced and reduced (P0.01), and the immediate moxibustion group was in advance. Compared with the pre moxibustion group, the latent period of the dysmenorrhea was significantly prolonged (P0.01). Compared with the immediate moxibustion group, the latent period of the pre moxibustion group in advance was significantly longer than that in the immediate moxibustion group. The number of twisting bodies and the total twisting body score decreased and decreased significantly (P0.05 or P0.01). The moxibustion of the moxibustion group was not simultaneously moxibustion on the uterine tissue cells of the cold and wet PD rats. The effect of Pathomorphology on HE staining of uterus tissue was placed under the light microscope. General morphological observation showed that the morphology of uterus in the control group was regular, no edema and large number of neutrophils; the model group of rats had more regular tissue morphology, severe edema, hyperemia, large amount of neutrophils and lymphocytes; pre moxibustion group, immediate moxibustion group, pre moxibustion group, pre moxibustion group In the first instant moxibustion group, the tissues of the uterus of the rats were all morphologic rules, the tissue was slightly edema, no hyperemia, a large number of neutrophils, and a small amount of lymphocyte.3 did not simultaneously moxibustion on the MVD value of the uterus tissue of the cold wet and stagnant PD rats. The MVD of the uterus tissue of the rat model group was significantly reduced, compared with the control group, the difference was statistically significant (P0.05); moxibustion group in advance was in advance. In the instant moxibustion group, compared with the model group, the MVD of the uterus tissue of the 3 moxibustion groups was significantly increased, and the difference was statistically significant (P0.01). Compared with the pre moxibustion group in advance, the immediate moxibustion group was significantly more MVD than the pre moxibustion group (P0.05). The immediate moxibustion group was compared with the pre moxibustion group, and the immediate moxibustion group was more MVD than the pre moxibustion group. Although there were numerical differences, there was no statistical difference (P0.05). Compared with the immediate moxibustion group, the pre moxibustion group had the difference between the immediate moxibustion group and the immediate moxibustion group, but there was no statistical difference (P0.05) the effect of moxibustion on the plasma TXB2 and 6-keto-PGF1 alpha in the plasma of the cold wet and stagnant PD rats (.4) and the plasma TXB2 content and TXB2 in the model group of Dysmenorrhea Rats The ratio of /6-keto-PGF1 alpha was significantly increased, and the content of 6-keto-PGF1 a decreased significantly. Compared with the control group, the difference was statistically significant (P0.01). The prognosis of the moxibustion and moxibustion group, the immediate moxibustion group, the TXB2/6-keto-PGF1 alpha ratio of the plasma TXB2 and the TXB2/6-keto-PGF1 alpha in the pre moxibustion group were significantly decreased, the content of 6-keto-PGF1 alpha was significantly increased, and the model group was significantly higher. Compared, the difference was statistically significant (P0.01); compared with the pre moxibustion group, the plasma TXB2 content and TXB2/6-keto-PGF1 alpha ratio in the pre moxibustion group in advance were significantly lower than that in the pre moxibustion group, and the content of 6-keto-PGF1 a was significantly increased (P0.01 or P0.05); that is, the immediate moxibustion group was compared with the pre moxibustion group, and the immediate moxibustion group was more than the pre moxibustion group blood. There was no significant difference in plasma TXB2,6-keto-PGF1 alpha content and TXB2/6-keto-PGF1 alpha ratio, but there was no statistical significance (P0.05). Compared with immediate moxibustion group and immediate moxibustion group, the plasma TXB2 content and TXB2/6-keto-PGF1 alpha ratio of rats in the immediate moxibustion group were significantly lower than those in the immediate moxibustion group, and the content of 6-keto-PGF1 a significantly increased (P0.01).5 not simultaneously. The effect of moxibustion on the PGF2 alpha, PGE2 content and the ratio of PGF2 a /PGE2 in the uterus tissue of PD rats with cold and wet stagnation, the content of PGF2 alpha and the ratio of PGF2 alpha /PGE2 in the model group were significantly increased, the content of PGE2 decreased obviously. The difference was statistically significant (P0.01) compared with the control group (P0.01). The prognosis of moxibustion, moxibustion group, immediate moxibustion group, pre moxibustion group, precondition In the first instant moxibustion group, the content of PGF2 alpha and the ratio of PGF2 alpha /PGE2 were significantly decreased, and the content of PGE2 increased significantly. Compared with the model group, the difference was statistically significant (P0.01 or P0.05). Compared with the pre moxibustion group, the content of PGF2 A and the PGF2 a /PGE2 ratio of the uterus in advance moxibustion group were compared with those in the pre moxibustion group. Significantly lower (P0.01), the content of PGE2 increased significantly (P0.01). Compared with the pre moxibustion group, the content of PGF2 alpha and the ratio of PGF2 to /PGE2 in the immediate moxibustion group were significantly lower than those in the pre moxibustion group (P0.05) and the PGE2 content was significantly increased (P0.01). The advance moxibustion group was compared with the immediate moxibustion group, and the advance moxibustion group was compared with the engraved moxibustion group of the group of Dysmenorrhea Rats' uterus tissue PGF. The content of 2 alpha and the ratio of PGF2 alpha /PGE2 decreased significantly (P0.01), and the content of PGE2 increased significantly (P0.01).6. The effect of moxibustion on AVP content in the uterine tissue of cold wet and stagnant PD rats was significantly higher than that in the control group. The difference was statistically significant (P0.01) compared with the control group (P0.01). Moxibustion was a prognosis and moxibustion. Group, instant moxibustion group, in advance immediate moxibustion group 3 moxibustion groups of 3 groups of Dysmenorrhea Rats uterine tissue decreased significantly, and compared with the model group, the difference was statistically significant (P0.05 or P0.01). The pre moxibustion group, the immediate moxibustion group, the pre moxibustion group of 3 groups 22 compared, the 3 groups of uterine tissues, although there is a significant difference in AVP content, but no statistical significance (P0.05).7 is not The effect of moxibustion on ET-1 and NO content in uterus tissue of cold wet and stagnant PD rats increased significantly the content of ET-1 in uterus tissue of rats with dysmenorrhea, and the content of NO decreased significantly. Compared with the control group, the difference was statistically significant (P0.01). The prognosis of moxibustion and moxibustion, moxibustion group, immediate moxibustion group, and immediate moxibustion group of rat uterus tissue ET- The content of 1 was obviously decreased and the content of NO increased obviously. Compared with the model group, the difference was statistically significant (P0.01). Compared with the pre moxibustion group, the content of ET-1 in the pre moxibustion group in advance was significantly lower than that in the pre moxibustion group, and the content of NO increased significantly (P0.01). The immediate moxibustion group was compared with the pre moxibustion group, the immediate moxibustion group was compared with the pre moxibustion in the immediate moxibustion group. The content of ET-1 was obviously reduced, and the content of NO increased obviously (P0.05). Compared with the immediate moxibustion group, the content of ET-1 in the uterus tissue of the rats in advance moxibustion group was obviously lower than that in the immediate moxibustion group, and the content of NO was obviously increased (P0.05). Conclusion: 1 pre moxibustion, instant moxibustion and the intervention at different time of immediate moxibustion at different time can obviously reduce the cold dampness. The abdominal contraction and torsional body reaction in the stagnant PD rats obviously prolong the incubation period, but the effect is especially obvious in advance. It shows that moxibustion at different time has good analgesic effect on the rats of cold wet PD model, but the effect of immediate moxibustion with immediate immediate moxibustion can improve the cold dampness by three different kinds of moxibustion at different time. The uterine tissue congestion and edema state of the PD model rats, but there is no obvious difference between the 3 groups of pre moxibustion, immediate moxibustion and pre moxibustion,.3 three different time moxibustion can increase the MVD of the uterus tissue of the cold wet and stagnant PD model rats, which can improve the local microcirculation of the uterus. The effect of the first instant moxibustion group is the best, obviously better than the pre moxibustion and instant moxibustion. The effect may be through the warm effect of the fire, the heat transfer of the meridians and the transmission of nerve to the uterus, in order to increase the blood flow, change the local blood circulation, and then to alleviate the pain,.64 can reduce the cold wet PD mold with three different kinds of moxibustion. The content of plasma TXB2 and the ratio of TXB2/6-keto-PGF1 a, and the increase of the content of 6-keto-PGF1 a, especially the immediate moxibustion in advance, showed that moxibustion at different times had a good regulating effect on the above indexes, but the effect of immediate moxibustion was more significant. The mechanism of its action may be by regulating the activity of platelet. And vascular tension, improve the state of vasoconstriction, increase the local blood flow of the uterus, and achieve the purpose of relieving pain..5 three different moxibustion at different time can reduce the content of PGF2 alpha and the ratio of PGF2 a /PGE2 in the uterus tissue of PD model rats with cold damp and stagnation, and improve the level of PGE2, but the effect is especially obvious in advance moxibustion. At the same time, moxibustion has a good regulating effect on the abnormal level of PGF2 alpha and PGE2, and the effect of immediate moxibustion is more significant. Its mechanism may be to improve the endocrine environment of the body by restoring the balance of PGF2 alpha and PGE2, and then to inhibit the uterine smooth muscle and vascular spasmodic contraction and improve the local ischemic and anoxic state. Three different moxibustion at different times of.6 can obviously reduce the AVP content in the uterus tissue of PD model rats with cold dampness and stagnation, and there is no obvious difference between them. The mechanism may be to reduce the synthesis and release of PG by reducing the content of AVP, thus effectively inhibiting the spastic contraction of the uterine smooth muscle and improving the local blood circulation. Increase the blood flow, alleviate the ischemic state, and play its analgesic effect,.7 three different time moxibustion can reduce the content of ET-1 and increase the content of NO, in which the immediate moxibustion is the most obvious, the immediate moxibustion is the second. The mechanism may be by relaxing the uterine smooth muscle, inhibiting its contraction, improving the uterine tissue microcirculation and increasing its blood flow.
【学位授予单位】：河北医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R245

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