“长强”穴药线植入对肛门切口痛大鼠机械痛阈及脊髓磷酸化p 38丝裂原活化蛋白激酶的影响

发布时间：2018-06-15 18:01

本文选题：术后疼痛 + 穴位埋线　；参考：《针刺研究》2017年05期

【摘要】：目的:探讨"长强"穴药线植入对肛门部切口痛模型大鼠止痛的作用机制。方法:SD大鼠随机分为空白组、模型组、长强穴药线植入组、非经非穴药线植入组,每组10只。建立肛门部切口痛模型,长强穴药线植入组用药线植入"长强"穴,非经非穴药线植入组用药线植入胁下非经非穴点。各组于术前及术后4、8、12、24h用Von Frey纤维丝测定大鼠术区及肛门左侧距术区15 mm处伤害性机械刺激的回缩阈值(MWT)。采用免疫组化SP法检测脊髓磷酸化p 38丝裂原活化蛋白激酶(p-p 38MAPK)水平。结果:与空白组比较,模型组术区及距术区15mm处MWT在术后4、8、12、24h均明显降低(P0.05),脊髓p-p 38MAPK水平明显升高(P0.05)。与模型组比较,长强穴药线植入组术区MWT在术后8、12、24h明显升高(P0.05),距术区15mm处MWT在术后12、24h明显升高(P0.05),术后24h脊髓p-p 38 MAPK水平明显降低(P0.05)。非经非穴药线植入组术区及距术区15 mm处MWT、术后24h脊髓p-p 38MAPK水平与模型组比较,差异均无统计学意义(P0.05)。结论:"长强"穴药线植入能显著提高肛门部切口痛模型大鼠的机械痛阈,其机制可能与"长强"药线植入降低切口痛大鼠脊髓p-p 38 MAPK水平有关,非经非穴药线植入不具有相同作用。
[Abstract]:Objective: to investigate the mechanism of analgesic effect of "Changqiang" acupoint line implantation on anus incision pain model rats. Methods Sprague-Dawley rats were randomly divided into blank group, model group, Changqiang acupoint line implantation group and non-meridian non-acupoint drug line implantation group with 10 rats in each group. The anus incision pain model was established. The "Changqiang" point was implanted into the drug line of Changqiang acupoint implantation group, and the non-meridian non-point was implanted under the hypochondria in the non-meridian drug line implantation group. Von Frey filament was used to determine the retraction threshold of nociceptive mechanical stimulation at 15 mm from the operation area and the left anal area in each group. The level of p38 mitogen-activated protein kinase (pp38 MAPK) in spinal cord was detected by immunohistochemical SP method. Results: compared with the blank group, the level of p-p38 MAPK in the spinal cord increased significantly in the model group and in the 15mm area after operation. Compared with the model group, the level of p-p38 MAPK in the spinal cord decreased significantly at 24 hours after operation in the Changqiang acupoint line implantation group, and at 24 hours after the operation, the level of p-p38 MAPK in the spinal cord decreased significantly at 24 hours after the operation, and at 24 hours after the operation, the level of p-p38 MAPK in the spinal cord decreased significantly. There was no significant difference in the level of p-p38 MAPK between the two groups 24 hours after operation compared with the model group. There was no significant difference in the level of p-p38 MAPK between the two groups (P 0.05). Conclusion: "Changqiang" drug line implantation can significantly increase the mechanical pain threshold of rats with anal incision pain, and the mechanism may be related to the decrease of p-p38 MAPK level in spinal cord of "Changqiang" group. Non-meridian non-acupoint line implantation does not have the same effect.
【作者单位】：锦州医科大学附属第三医院;锦州医科大学;辽宁石化职业技术学院;
【基金】：辽宁省大学生创新创业训练计划项目(No.201510160000020)
【分类号】：R245

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