“益气活血”法治疗破裂型腰椎间盘突出症的实验研究

发布时间：2018-06-20 09:01

本文选题：益气活血方 + TXB_2/PGF_(1α)　；参考：《广西中医药大学》2017年硕士论文

【摘要】：目的:1)以p38MAPK信号转导通路作为切入点,探讨破裂型腰椎间盘突出模型大鼠基于p38MAPK的磷酸化与靶标的腰间盘组织中表达MMP-7和MMP-3蛋白相互影响的关系。2)观察益气活血方对裂型腰椎间盘突出大鼠椎间盘组织p38MAPK信号转导通路的影响,初步探讨其改善的分子机制。方法:建立腰椎间盘突出重吸收大鼠模型,大鼠随机分为模型组、益气活血方组、益气活血方+P38抑制剂组和P38抑制剂组,另设假手术组(n=10),假手术组及模型组灌胃纯净水,其他各组大鼠分别灌胃相应的药物,干预3周,3周后麻醉处死大鼠,采用双抗体夹心法(ELISA)、HE和Western-Blot法测定血清中TXB_2和PGF_(1α)的含量和椎间盘组织病理学改变,以及椎间盘组织中P-p38MAPK及MMP-3、MMP-7蛋白表达。结果:1)模型组大鼠腰间盘组织可见有间质轻度水肿,并有少许血管增多,单核炎细胞浸润及少量纤维组织增生,部分可见有神经萎缩。给予益气活血方治疗后,其病程均有所改善。2)益气活血方(“益气活血”法代表方)干预破裂型腰椎间盘突出模型大鼠后,结果显示血清中TXB_2含量明显下降,PGF_(1α)含量有所升高,而TXB_2/PGF_(1α)比值明显下降,均向良性转化的趋势;与模型组相比较(P值均0.01),结果具有显著性差异。3)与模型组比较,益气活血方组P-p38MAPK和MMP-3、MMP-7蛋白表达均增高,结果具有显著性差异(P0.05或P0.01);与抑制剂组比较,益气活血方+抑制剂组P-p38MAPK和MMP-3、MMP-7蛋白表达均增高,结果具有显著性差异(P0.05或P0.01)。结论:益气活血方治疗后病变程度有所改善,其作用机制可能激活p38MAPK信号通路而提高椎间盘组织MMP-3、MMP-7蛋白表达有关。
[Abstract]:Objective: to use p38 MAPK signal transduction pathway as the entry point. To investigate the relationship between phosphorylation of p38 MAPK and the expression of MMP-7 and MMP-3 protein in the target lumbar disc of rat model of ruptured lumbar intervertebral disc herniation. 2) to observe the effect of Yiqi Huoxue recipe on intervertebral disc tissue of rat with cleft lumbar intervertebral disc herniation. The effect of p38 MAPK signal transduction pathway, The molecular mechanism of its improvement was preliminarily discussed. Methods: a rat model of reabsorption of lumbar disc herniation was established. Rats were randomly divided into three groups: model group, P38 inhibitor group and P38 inhibitor group. Rats in other groups were administrated with corresponding drugs respectively. After 3 weeks and 3 weeks of intervention, the rats were killed under anesthesia. The contents of TXBStack2 and PGFS-1 伪 in serum and histopathological changes of intervertebral disc were measured by double antibody sandwich method and Western-Blot method. And the expression of P-p38 MAPK and MMP-3-7 protein in intervertebral disc tissue. Results in the model group, there were slight edema in the lumbar intervertebral disc, a little increase in blood vessels, infiltration of mononuclear cells and hyperplasia of a few fibrous tissues, and some neuroatrophy was found in some of the rats in the model group. After the treatment of Yiqi Huoxue recipe, the course of disease was improved. 2) after the intervention of Yiqi Huoxue recipe ("Yiqi Huoxue" method), the results showed that the content of TXBS-2 in serum decreased obviously and the content of PGFS-1 伪 increased. Compared with the model group, the ratio of TXB _ 2 / PGF _ (1 伪) was significantly decreased, and the expression of P-p38 MAPK and MMP-3 / MMP-7 in the Yiqi Huoxue recipe group was significantly higher than that in the model group, and the P value was 0.01 in comparison with the model group, and the expression of MMP-3 and MMP-3 was significantly higher in the Yiqi Huoxue recipe group than in the model group. Results compared with the inhibitor group, the expression of P-p38 MAPK and MMP-3 MMP 7 in the Yiqi Huoxue prescription inhibitor group was significantly higher than that in the control group. The results showed that there was significant difference between the two groups (P0.05 or P0.01). Conclusion: the pathological degree of Yiqi Huoxue prescription is improved after treatment, and its mechanism may be related to the activation of p38 MAPK signaling pathway and the increase of MMP-3 and MMP-7 protein expression in intervertebral disc tissue.
【学位授予单位】：广西中医药大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R274.9

【参考文献】