针刺对海洛因成瘾大鼠脑损伤神经保护作用

发布时间：2018-06-20 09:39

本文选题：针刺 + 内质网应激反应　；参考：《中医药临床杂志》2017年01期

【摘要】：目的:观察大鼠脑海马区和中脑腹侧被盖区(ventral tegmental area,VTA)细胞凋亡情况、活化转录因子-6(activating transcription factor-6,ATF-6)、X-盒结合蛋白1(X-box binding protein,XBP1)的基因表达以及ATF6、XBP1蛋白表达的影响,分析针刺对内质网应激反应的调控作用,探讨针刺调控ATF6、XBP1,对海洛因成瘾大鼠脑损伤的神经保护作用。方法:54只SD大鼠,全部选用雄性大鼠,将大鼠随机分为3组:对照组、模型组、针刺组,每组18只。通过持续8天肌肉注射递增量海洛因,建立海洛因成瘾大鼠模型。在按照染毒(成瘾)→脱毒的实验方法,建立海洛因复吸大鼠模型。于实验第39天取各组大鼠海马和VTA组织。采用TUNEL方法检验海马区和VTA区细胞凋亡的改变。ATF6、XBP1蛋白的表达采用免疫组化方法检验。ATF6、XBP1m RNA采用荧光定量RTPCR检验。结果:经过海洛因复吸造模以后,模型组、针刺组海马区和VTA区阳性神经细胞总数目较对照组明显增多(P0.01);与模型组比,针刺组阳性神经细胞总数目明显减少(P0.01)。模型组海马区和VTA区ATF6、XBP1蛋白及其m RNA的表达较对照组比明显升高(P0.01);与模型组比,针刺组海马和VTA区ATF6、XBP1蛋白及其m RNA的表达明显降低(P0.05)。结论:针刺通过抑制ATF6和XBP1表达上调,减少神经细胞凋亡,可能是针刺干预海洛因复吸脑损伤的主要作用之一。
[Abstract]:Objective: To observe the apoptosis of the ventral tegmental area (VTA) cells in the hippocampus and the ventral mesencephalon of the rat, the expression of the activation transcription factor -6 (activating transcription factor-6, ATF-6), the gene expression of X- box binding protein 1 (X-box binding) and the effect of the protein expression, and to analyze the stress response to the endoplasmic reticulum. The neuroprotective effect of acupuncture on ATF6, XBP1, and the brain damage of heroin addicted rats was studied. Methods: 54 SD rats were selected and randomly divided into 3 groups: the control group, the model group, the acupuncture group, 18 rats in each group. The heroin addicted rat model was established through the continuous 8 days intramuscular injection of heroin. The rat model of heroin relapse was established according to the experimental method of detoxification (Cheng Yin) and detoxification. The hippocampus and VTA tissues of rats in each group were taken on the thirty-ninth day of the experiment. The apoptosis of hippocampal and VTA regions was examined by TUNEL. The expression of XBP1 protein was examined by immunohistochemical method and.ATF6, and XBP1m RNA was tested by fluorescence quantitative RTPCR test. The results were as follows: After heroin relapse, the total number of positive neurons in the hippocampus and VTA area of the model group and the acupuncture group increased significantly (P0.01). Compared with the model group, the total number of positive nerve cells in the acupuncture group decreased significantly (P0.01). The expression of ATF6, XBP1 protein and m RNA in the hippocampus and VTA regions of the model group was significantly higher than that in the control group (P0.01). Compared with the model group, the expression of ATF6, XBP1 protein and m RNA in the hippocampus and VTA area of the acupuncture group decreased significantly (P0.05). Conclusion: acupuncture can inhibit the up regulation of ATF6 and XBP1 expression and reduce the apoptosis of nerve cells. It may be one of the main effects of acupuncture intervention on heroin relapse brain injury.
【作者单位】：安徽中医药大学针灸骨伤临床学院;安徽中医药大学针灸经络研究所;安徽中医药大学中西医结合临床学院;
【基金】：国家自然科学基金委员会面上项目(NO:81173325)
【分类号】：R245

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