益气血法对超排卵小鼠卵巢颗粒细胞Bcl-2、Bax、Caspase3表达的影响

发布时间：2018-06-21 07:13

本文选题：益气血法 + 经后增殖方　；参考：《实用医学杂志》2017年13期

【摘要】：目的观察经后增殖方对超排卵小鼠卵巢凋亡相关基因及其蛋白表达的调节作用,探讨中药益气血法对超排卵小鼠卵巢颗粒细胞凋亡及卵泡质量的影响。方法建立超排卵小鼠模型,30只小鼠随机分为空白组、模型组、中药治疗组。采用实时荧光定量PCR(real-time quantitative PCR,qPCR)和免疫印迹法(Western blot)分别检测Bcl-2、Bax、Caspase3基因和蛋白的表达水平。结果中药治疗组Bax、Cas-pase3 mRNA的表达水平与模型组比较(0.895 vs 1.779,0.674 vs 2.066,均P0.05),差异均有统计学意义;中药治疗组Bcl-2、Bax、Caspase3 mRNA的表达水平与空白组比较(1.11 vs 1.26,0.89 vs 0.79,0.67 vs 0.96),差异均无统计学意义(P0.05);中药治疗组Bcl-2、Bax、Caspase3蛋白的表达水平与模型组比较(0.62 vs0.41,0.79 vs 1.06,0.49 vs 0.89),差异均有统计学意义(P0.05),与空白组比较(0.62 vs 0.64,0.79 vs 0.73,0.49 vs 0.50)差异均无统计学意义(P0.05)。结论经后增殖方能促进Bcl-2蛋白的表达,抑制Bax、Cas-pase3基因及蛋白的表达,抑制超排卵小鼠卵巢颗粒细胞凋亡至接近自然排卵小鼠的水平,提高超排卵小鼠卵泡质量。
[Abstract]:Objective to observe the effect of Jinghou recipe on the expression of ovarian apoptosis-related genes and proteins in superovulation mice, and to explore the effect of Yiqi and Xue method on ovarian granulosa cell apoptosis and follicle quality in superovulation mice. Methods Thirty mice with hyperovulation were randomly divided into blank group, model group and traditional Chinese medicine treatment group. The expression of caspase3 gene and protein were detected by real-time quantitative qPCR and Western blotting, respectively. Results compared with the model group, the expression level of Bax-Caspase3 mRNA in the traditional Chinese medicine treatment group was 0.895 vs 1.779c0.674 vs 2.066, all the differences were statistically significant (P 0.05). The expression level of Bcl-2OBax-Caspase3 mRNA in the TCM treatment group was higher than that in the control group (1.11 vs 1.260.89 vs 0.79U 0.67 vs 0.96), and there was no significant difference in the expression of Bcl-2Bax-Caspase3 protein between the Chinese medicine group and the model group (0.62 vs 0.41 vs 1.06 卤0.49 vs 0.89, respectively), and the difference was statistically significant (P 0.05P 0.05A). The expression of Bcl-2AXCaspase3 mRNA in the treatment group was significantly higher than that in the control group (P 0.05). Compared with the control group, there was no significant difference in 0.62 vs 0.64N 0.79 vs 0.73N 0.49 vs 0.50). Conclusion the prescription can promote the expression of Bcl-2 protein, inhibit the expression of Bax-Cas-pase3 gene and protein, inhibit the apoptosis of ovarian granulosa cells to the level of natural ovulation mice, and improve the follicle quality of superovulation mice.
【作者单位】：广州军区广州总医院生殖医学中心;广州中医药大学;汕头大学医学院附属第一医院康复科;
【基金】：国家自然科学基金资助项目(编号:81403249)
【分类号】：R271.9

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