针刺对乳腺增生大鼠乳腺组织Fasl、Fas表达的影响

发布时间：2018-06-22 14:02

本文选题：乳腺增生 + 针刺疗法　；参考：《中华中医药学刊》2017年11期

【摘要】：目的:探究针刺对乳腺增生大鼠乳腺组织中Fasl和Fas蛋白表达的影响。方法:选取40只SPF级健康雌性未孕SD大鼠,12周龄,随机分为4组:正常组、模型组、针刺组、三苯氧胺组,每组10只。经25 d造模成功后,进行4周的干预治疗。HE染色法对乳腺组织进行病理学观察;免疫组化法、Western Blot法检测各组大鼠乳腺组织Fasl、Fas的蛋白表达情况。结果:与正常组相比,模型组乳腺小叶显著增多,腺泡结构紊乱,组织增生现象明显,Fas蛋白表达水平明显降低(P0.01),Fasl蛋白表达水平明显增高(P0.01);经治疗后,针刺组与三苯氧胺组较模型组小叶数量减少,腺泡排列较为整齐,组织增生的病理变化减轻,Fas蛋白表达水平明显增高(P0.05或P0.01),Fasl蛋白表达水平明显降低(P0.05或P0.01)。结论:针刺能够干预乳腺增生大鼠乳腺组织中Fasl和Fas蛋白表达情况,对乳腺增生大鼠有调节和治疗作用。
[Abstract]:Aim: to investigate the effect of acupuncture on the expression of Fasl and FAS protein in mammary gland of rats with mammary hyperplasia. Methods: forty healthy female SD rats of SPF grade were randomly divided into 4 groups: normal group, model group, acupuncture group and tamoxifen group. After 25 days of successful modeling, the rats were treated for 4 weeks. The histopathology of mammary gland was observed by HE staining, and the expression of Faslfas protein in mammary gland of rats in each group was detected by Western blot. Results: compared with the normal group, the expression of FAS protein in the mammary lobule of the model group was significantly increased, the acinar structure was disordered, the tissue proliferation was obviously decreased (P0.01) and the expression of Fasl protein was significantly increased (P0.01), after treatment, the expression of FAS protein was significantly increased (P0.01). Compared with the model group, the number of lobules in acupuncture group and tamoxifen group were decreased, the acinar arrangement was neatly arranged, and the pathological changes of tissue proliferation reduced the expression level of FAS protein (P0.05 or P0.01) significantly lower than that of model group (P0.05 or P0.01). Conclusion: acupuncture can interfere with the expression of Fasl and FAS protein in mammary gland of rats with hyperplasia of mammary gland, and can regulate and treat the expression of Fasl and FAS in rats with hyperplasia of mammary gland.
【作者单位】：辽宁中医药大学研究生学院;辽宁中医药大学中医脏象理论及应用教育部重点实验室;辽宁中医药大学第一临床学院;
【基金】：国家自然科学基金面上项目(81273698) 辽宁省科学技术基金面上项目(20170540612) 辽宁省科学事业公益研究基金项目(2014001021) 辽宁中医药大学中医脏象理论及应用国家教育部重点实验室开放基金项目(zyzx1609);辽宁中医药大学大学生创新创业训练计划项目(201610162034)
【分类号】：R245

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