针药结合对盆腔炎性疾病后遗症大鼠血清中IL-6、IL-8表达的影响
发布时间:2018-06-23 01:13
本文选题:盆腔炎性疾病后遗症 + 正虚邪恋 ; 参考:《黑龙江中医药大学》2017年硕士论文
【摘要】:目的:通过观察针刺结合盆舒饮对盆腔炎性疾病后遗症大鼠血清中促炎性细胞因子IL-6、IL-8的含量影响,探讨其治疗盆腔炎性疾病后遗症的作用机制及效果优势。方法:1、选用Wistar雌性大鼠作为受试对象,采用"机械损伤+混合菌感染"的方法建立盆腔炎性疾病后遗症大鼠模型。分别于造模后第7、14、21天对大鼠子宫组织进行病理学观察。2、随机抽取8只大鼠作为空白组,将其余大鼠进行造模,造模后的大鼠随机分成5组,于造模后第15天进行干预治疗。空白对照组和模型组都给予蒸馏水灌胃,针刺组选取腧穴关元、血海和三阴交进行治疗,中成药组以丹黄去瘀胶囊悬液灌胃,中药组以盆舒饮汤剂进行灌胃、针药结合组大鼠给予针刺刺激同时联合盆舒饮灌胃治疗,14天后,采用HE染色法,通过光镜对大鼠子宫组织病理改变进行观察;用ELISA法检测大鼠血清中的促炎性因子IL-6、IL-8的浓度。结果:1、成功建立盆腔炎性疾病后遗症大鼠模型。通过观察发现:造模术后7天,大鼠子宫组织明显充血,炎细胞浸润,无盆腔粘连、纤维化改变等典型慢性炎症表现;造模术后14天,肉眼观察大鼠盆腔组织广泛粘连,子宫积水严重,光镜下可见腺体扩张,纤维化改变明显;IL-6、IL-8在SPID模型大鼠血清中的表达明显高于正常大鼠,p0.01。2、经过两周干预治疗后,各治疗组大鼠盆腔情况均有不同程度好转,其中以针刺结合盆舒饮组改善程度最佳;各治疗组大鼠血清中IL-6、IL-8水平均有所下调,其中以针刺联合盆舒饮组下降最明显,与正常对照组比较,p0.05。结论:通过"机械损伤+混合菌感染"的方法可成功建立SPID大鼠模型。针刺联合盆舒饮主要通过抑制炎症大鼠血清中的促炎性细胞因子IL-6、IL-8的表达,来减轻组织损伤,促进炎症吸收,从而达到治疗盆腔炎性疾病后遗症的目的。
[Abstract]:Objective: To observe the effect of acupuncture combined with basin Shu Yin on the serum levels of proinflammatory cytokines IL-6 and IL-8 in the serum of the sequelae of pelvic inflammatory disease in rats, and to explore the mechanism and effect of its effect on the sequelae of pelvic inflammatory disease. Methods: 1, the female rats of Wistar were selected as subjects, and "mechanical injury + mixed bacteria infection" was used. The rat model of the sequelae of pelvic inflammatory disease was established by the method..2 was observed on the uterus tissue of the rats on day 7,14,21 after the model. 8 rats were randomly selected as the blank group. The other rats were made into the model. The rats were randomly divided into 5 groups, and the intervention treatment was carried out at fifteenth days after the model. The blank control group and the model group were all given. The acupuncture group was given the stomach with distilled water, the acupuncture group selected acupoint Guanyuan, Xuehai and Sanyinjiao for treatment. The Chinese patent medicine group was filled with Dan Huang Qi stasis capsule suspension, and the Chinese medicine group was treated with basin Shu Yin decoction. The acupuncture and drug combination group was given acupuncture stimulation at the same time with basin Shu Yin to fill the stomach. After 14 days, the uterus tissue of rats was used by the HE staining method. The pathological changes were observed and the concentration of proinflammatory factor IL-6 and IL-8 in the serum of rats was detected by ELISA. Results: 1, the rat model of the sequelae of pelvic inflammatory disease was successfully established. Through observation, it was found that the uterus tissue was obviously congested, inflammatory cell infiltration, no pelvic adhesions, fibrosis changes and other typical chronic inflammatory manifestations in the rat uterus on the 7 day after the operation. 14 days after the operation, the pelvic tissue was widely adhered by the naked eye, the hydrops of the uterus were serious, the glandular expansion was seen under the light microscope, and the fibrosis changes were obvious. The expression of IL-6, IL-8 in the serum of SPID model rats was significantly higher than that of the normal rats, p0.01.2. After two weeks of intervention, the pelvic cavity of the rats in each treatment group improved in varying degrees. The improvement of the combination of acupuncture combined with basin Shu Shu group was the best. The average decrease of IL-6 and IL-8 water in the serum of the rats in each treatment group was down, with the most obvious decrease in the acupuncture combined with the basin Shu Shu group, and compared with the normal control group. P0.05. conclusion: the SPID rat model could be successfully established through the method of "mechanical injury + mixed bacteria infection". The expression of pro-inflammatory cytokine IL-6 and IL-8 in the serum of inflammatory rats can reduce tissue damage and promote inflammatory absorption, thus achieving the purpose of treating the sequelae of pelvic inflammatory disease.
【学位授予单位】:黑龙江中医药大学
【学位级别】:硕士
【学位授予年份】:2017
【分类号】:R271.9
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