不同归经补益中药对骨转移癌CXCR4表达的影响

发布时间：2018-06-26 09:53

本文选题：骨趋化因子受体 + 补益中药　；参考：《中国中西医结合杂志》2017年05期

【摘要】：目的观察不同归经补益中药对骨转移癌骨趋化因子受体4(CXCR4)的抑制及机制。方法采用转移性大鼠乳腺癌细胞,按Medhurst方法复制骨转移癌模型。40只大鼠按照体重分层,采用随机数字表法分为假手术组、模型组、肾经组、肺经组和肝经组,每组8只。造模后肾经组(补骨脂、肉苁蓉及益智仁)、肝经组(熊胆粉)、肺经组(党参、黄芪及百合)给药量为10 mL/(kg·d),相当于生药45 g/(kg·d)灌胃,假手术组及模型组给予去离子水灌胃,持续20天。观察不同归经补益中药的作用,通过影像学方法进行评分、测量肿瘤体积,HE染色观察形态,免疫组化法检测CXCR4表达并进行半定量分析。结果骨转移癌模型出现骨质破坏和肿瘤生长等表现。与假手术组比较,模型组肿瘤体积增大(P0.01),模型组骨质破坏严重,评分升高(P0.01),CXCR4表达升高(P0.01)。与模型组比较,肾经组肿瘤体积缩小(P0.05),骨质破坏明显减轻,评分降低(P0.05),CXCR4表达明显下降(P0.01);肺经组和肝经组上述指标与模型组比较,差异均无统计学意义(P0.05)。结论归肾经中药对骨转移癌灶的生长具有一定的抑制作用,其机制可能通过阻断SDF-1/CXCR4生物学轴及下游信号通路,达到抗肿瘤定向侵袭和增殖的目的。归肺经、归肝经药物不存在类似作用,不同归经中药对机体的作用存在靶向特异性。
[Abstract]:Objective to observe the inhibition of CXCR4 on bone chemokine receptor 4 (CXCR4) in patients with bone metastases. Methods the metastatic rat breast cancer cells were used. According to Medhurst's method, 40 rats were divided into sham-operation group, model group, renal meridian group, lung meridian group and liver meridian group with 8 rats in each group. The dosage of 10 mL / (kg d), in kidney meridian group (psoralen, Cistanche deserticola and Yizhiren), liver meridian group (bear gallbladder powder) and lung meridian group (Codonopsis pilosula, astragalus membranaceus and lily) was equivalent to 45 g / (kg d) of raw medicine, and the sham operation group and model group were given deionized water. It lasts for 20 days. The effects of different meridians and tonifying herbs were observed, the tumor volume was measured by HE staining, the expression of CXCR4 was detected by immunohistochemistry and semi-quantitative analysis was carried out. Results Bone destruction and tumor growth were found in the model of bone metastases. Compared with the sham-operated group, the tumor volume in the model group increased (P0.01), the bone destruction was serious in the model group, and the expression of CXCR4 in the model group was increased (P0.01). Compared with the model group, the tumor volume of kidney meridian group was reduced (P0.05), bone destruction was alleviated significantly, and the score decreased (P0.05). The expression of CXCR4 in lung meridian group and liver meridian group was significantly lower than that in model group (P0.01). There was no significant difference between lung meridian group and liver meridian group compared with model group (P0.05). Conclusion Guishen meridian has a certain inhibitory effect on the growth of bone metastases, and its mechanism may be through blocking the biological axis and downstream signal pathway of SDF-1 / CXCR4 to achieve the purpose of anti-tumor directed invasion and proliferation. There is no similar effect in Gui Fei meridian and Guigan meridian drugs, but there is a target specificity in the effect of different Gui Jing traditional Chinese medicine on the body.
【作者单位】：中国中医科学院望京医院肿瘤科;
【基金】：国家自然科学基金资助项目(No.81173374)
【分类号】：R273

【相似文献】