不同频率电针对脑梗死大鼠缺血区UCP5表达影响的研究

发布时间：2018-07-05 19:59

本文选题：脑梗死 + 大鼠　；参考：《黑龙江中医药大学》2017年硕士论文

【摘要】：目的:通过观察不同频率电针对脑梗死大鼠脑内缺血区解偶联蛋白5(uncoupling protein 5,UCP5)表达的影响,以探讨电针治疗脑梗死的中枢机制。方法:将健康成年雄性SD大鼠60只,随机分为空白组、假手术组、模型组、2Hz电针组、50Hz电针组和100Hz电针组,每组10只。模型组和不同频率的电针组大鼠应用线栓法制备大脑中动脉闭塞脑缺血模型。空白组、假手术组及模型组大鼠不予治疗,仅进行绑缚处理。电针组取大鼠患侧"前三里穴"和"外关穴",分别给予2Hz、50Hz和100Hz,连续波电针治疗,每次20分钟,每日1次,连续治疗21天。每组大鼠均在术后第1、3、7、14、21天,运用网屏试验进行行为学评分,以检测大鼠的神经缺损情况。各组大鼠在第21天对其右侧大脑皮层组织进行取材,运用免疫组化法检测大鼠脑缺血区UCP5阳性表达的情况。研究结束后采用SPSS20.0统计软件对所得数据进行统计分析。结果:1.行为学评分结果显示:2Hz组与模型组、50Hz组、100Hz组相比:术后21天,2Hz组大鼠运动神经功能优于模型组,差异具有统计学意义(P0.05)。50Hz组与模型组、2Hz组、100Hz组相比:术后7天,50Hz组与模型组相比,50Hz组大鼠运动神经功能优于模型组,差异有统计学意义(P0.01);术后14天,50Hz组与其他各组相比,50Hz组大鼠运动神经功能均优于其他各组,差异存在统计学意义(P0.05);术后21天,50Hz组与其他各组相比,50Hz组大鼠运动神经功能均优于其他各组,差异存在统计学意义(P0.01)。2.大鼠大脑皮层UCP5阳性表达结果显示:空白组与假手术组、100Hz组比,差异无统计学意义(P0.05)。模型组与各电针组相比,差异无统计学意义(P0.05);模型组与空白组相比,模型组大鼠脑保护作用优于空白组,差异具有统计学意义(P0.01)。不同频率电针组与其他组相比较:①2Hz组与空白组、50Hz组、100Hz组相比:其中2Hz组与空白组相比,2Hz组大鼠脑保护作用优于空白组,差异有统计学意义(P0.01);2Hz组与50Hz组相比,差异无统计学意义(P0.05);2Hz与100Hz组相比,2Hz组大鼠脑保护作用优于100Hz组,差异具有统计学意义(P0.05)。②50Hz组与空白组、100Hz组相比:其中50Hz组与空白组相比,50Hz组大鼠脑保护作用优于空白组,差异具有统计学意义(P0.01);50Hz组与100Hz组相比,差异无统计学意义(P0.05)。结论:1.电针能够改善脑梗死大鼠运动神经功能的缺损情况,并促进其上肢运动功能的恢复。2.电针治疗对于脑梗死具有脑保护的作用,其机制可能与其对缺血区UCP5的上调有关,不同频率的电针均能够上调UCP5的表达,其中2Hz电针组效果最显著,其次为50Hz和100Hz电针组。
[Abstract]:Objective: to investigate the central mechanism of electroacupuncture in the treatment of cerebral infarction by observing the effect of different frequency electroacupuncture on the expression of uncoupling protein 5 (UCP5) in ischemic area of rats with cerebral infarction. Methods: sixty healthy adult male SD rats were randomly divided into three groups: blank group, sham operation group, model group, 2 Hz electroacupuncture group and 100 Hz electroacupuncture group, with 10 rats in each group. Middle cerebral artery occlusion (MCAO) model was established in model group and electroacupuncture group with different frequency. Blank group, sham operation group and model group rats were not treated, only tied treatment. In the electroacupuncture group, the "first three li points" and "Waiguan points" in the affected side of rats were treated with 2 Hz and 100 Hz respectively, continuous wave electroacupuncture for 20 minutes, once a day, for 21 days. The rats in each group were assessed with screen test on the first day after operation. The positive expression of UCP5 was detected by immunohistochemical method in the right cerebral cortex of rats on the 21st day. At the end of the study, SPSS 20.0 statistical software was used to analyze the data. The result is 1: 1. The behavioral score showed that the motor nerve function of the rats in the 2 Hz group was better than that in the model group 21 days after operation, compared with that in the 50 Hz group and the 100 Hz group. The difference was statistically significant (P0.05) .50Hz group compared with the model group (2Hz group) and 100Hz group: the motor nerve function of the 50Hz group was superior to that of the model group (P 0.05), and the motor nerve function of the 50Hz group was better than that of the model group. The motor nerve function in 50Hz group was better than that in other groups on the 14th day after operation (P0.01), and the motor nerve function in 50Hz group was better than that in other groups. The difference was statistically significant (P0.05); the motor nerve function in 50Hz group was better than that in other groups 21 days after operation (P0.01). 2. The positive expression of UCP5 in cerebral cortex of rats showed that there was no significant difference between the blank group and the sham operation group (P 0.05). There was no significant difference between the model group and each electroacupuncture group (P0.05); compared with the blank group, the model group had better brain protection than the blank group, the difference was statistically significant (P0.01). The brain protective effect of 2Hz group was better than that of blank group (P0.01), and the difference was statistically significant (P0.01) between 2Hz group and 50Hz group. There was no significant difference (P0.05) the brain protective effect of 2Hz group was better than that of 100Hz group compared with 100Hz group. The difference was statistically significant (P0.05) .250Hz group compared with the blank group (100Hz group): 50Hz group compared with the blank group compared with the control group (50Hz group) was better than the blank group (P < 0.05). The difference was statistically significant (P0.01). There was no significant difference between 50Hz group and 100Hz group (P0.05). Conclusion 1. Electroacupuncture can improve the defect of motor nerve function in rats with cerebral infarction and promote the recovery of motor function of upper limb. Electroacupuncture therapy has the protective effect on cerebral infarction, and its mechanism may be related to the upregulation of UCP5 in ischemic area. Electroacupuncture at different frequencies can up-regulate the expression of UCP5, especially in 2Hz electroacupuncture group, followed by 50Hz and 100Hz electroacupuncture group.
【学位授予单位】：黑龙江中医药大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R245

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