化瘀通络灸法对皮质下血管性痴呆大鼠额叶皮质下白质TNF-α、IL-1β表达的影响

发布时间：2018-07-17 07:04

【摘要】：目的观察化瘀通络灸法对皮质下血管性痴呆(subcortical vascular dementia,SVD)模型大鼠治疗作用及其对额叶皮质下白质炎性因子的影响。方法采用Pulsinelli4血管阻断法稍加改进复制大鼠血管性痴呆模型,电脑随机数字表法随机分为3组:艾灸组、药物组、模型组,并设假手术组、正常组对照。艾灸组取百会、大椎、神庭穴艾灸治疗,药物组多奈哌齐灌胃治疗。每日1次,连续治疗4周。采用跳台仪检测治疗后学习记忆成绩;采用免疫组化检测额叶皮质下白质肿瘤坏死因子-α(tumor necrosis factor-alpha,TNF-α)、白介素-1β(Interleukin-1beta,IL-1β)阳性细胞表达。结果 (1)大鼠跳台实验的潜伏期、错误次数,艾灸组及药物组与模型组比较差异具有统计学意义(P0.01,P0.05),且艾灸组明显优于药物组(P0.01,P0.05)。(2)艾灸组及药物组与模型组比,大鼠额叶皮质下白质TNF-α、IL-1β表达差异均有统计学意义。艾灸组与药物组比较TNF-α、IL-1β表达差异有统计学意义(P0.05)。结论化瘀通络灸法治疗SVD可能是通过抑制炎性细胞因子TNF-α、IL-1β的产生,从而改善大鼠认知功能而实现的。
[Abstract]:Objective to observe the therapeutic effect of Huayu Tongluo moxibustion on subcortical vascular dementia (subcortical vascular dementia) model rats and its effect on subcortical white matter inflammatory factors. Methods the vascular dementia model of rats was induced by Pulsinelli4 vascular occlusion. The rats were randomly divided into three groups: moxibustion group, drug group, model group, sham operation group and normal control group. The moxibustion group was treated with Baihui, Dazhui and Shenting points moxibustion. Once a day for 4 weeks. The expression of TNF- 伪 and interleukin-1 尾 (IL-1 尾) in the subcortical white matter of frontal lobe were detected by means of platform jumper, and the expression of TNF- 伪 and interleukin-1 尾 (IL-1 尾) in the subcortical white matter of frontal lobe were detected by immunohistochemistry. Results (1) the latency, the number of errors, the difference between the moxibustion group and the model group were statistically significant (P0.01P05), and the moxibustion group was superior to the drug group (P0.01P05). (2) and the drug group compared with the model group. There were significant differences in the expression of TNF- 伪 and IL-1 尾 in the subfrontal cortex of rats. There was significant difference in the expression of TNF- 伪 and IL-1 尾 between moxibustion group and drug group (P0.05). Conclusion the treatment of SVD by removing blood stasis and releasing collaterals and moxibustion may be achieved by inhibiting the production of inflammatory cytokine TNF- 伪 and IL-1 尾, thereby improving the cognitive function of rats.
【作者单位】：安徽中医药大学;安徽中医药大学针灸骨伤临床学院;新安医学教育部重点实验室;
【基金】：国家自然科学基金(81373744)
【分类号】：R245

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