民族药野蚕豆根抗心肌缺血和抗凝血作用研究
发布时间：2018-08-11 16:15
【摘要】：目的观察民族药野蚕豆根抗心肌缺血和抗凝血等心血管方面相关的药理活性,为进一步探究野蚕豆根的临床应用方向和开发野蚕豆根的药用价值提供依据。方法对野蚕豆根进行急性毒性评价后,采用毛细玻管法、小鼠断尾法以及血浆复钙试验探究野蚕豆根相关抗凝血活性;采用盐酸异丙肾上腺素诱导小鼠急性心肌缺血模型研究野蚕豆根对心肌缺血损伤的保护作用;采用角叉菜胶致小鼠气囊滑膜炎模型观察野蚕豆根的抗炎活性。结果野蚕豆根用药安全,能明显增加小鼠的凝血时间和出血时间,其水、甲醇和乙酸乙酯提取部位也均能显著延长体外兔血浆复钙时间,其中水提取部位时间延长最明显;且其能够显著降低小鼠急性心肌缺血模型中血清乳酸脱氢酶和丙二醛的含量,减少炎症模型中前列腺素E2的渗出。结论野蚕豆根在抗心肌缺血和抗凝血等心血管相关方面均表现出了一定的药理活性,值得对野蚕豆根在心血管方面的药效机制和临床应用作进一步的研究开发。
[Abstract]:Objective to observe the pharmacological activities of Vicia faba root in the cardiovascular aspects such as myocardial ischemia and anticoagulant, so as to provide the basis for further exploring the clinical application direction of wild broad bean root and the medicinal value of developing wild broad bean root. Methods the acute toxicity evaluation of Vicia faba root was carried out, and the relative anticoagulant activity of Vicia faba root was investigated by capillary tube method, mouse tail cut method and plasma recalcium test. The protective effect of Vicia faba root on myocardial ischemia injury was studied in mice induced by isoproterenol hydrochloride, and the anti-inflammatory activity of Vicia faba root was observed by carrageenin induced mouse balloon synovitis model. Results the drug of Vicia faba root was safe and could significantly increase the blood coagulation time and bleeding time of mice. The water, methanol and ethyl acetate extracts could also significantly prolong the recalcium time of rabbit plasma in vitro, among which the time of water extraction was the most obvious. It can significantly reduce the contents of serum lactate dehydrogenase and malondialdehyde in mice with acute myocardial ischemia and decrease the exudation of prostaglandin E _ 2 in inflammatory model. Conclusion Vicia faba root has some pharmacological activities in cardiovascular related aspects such as myocardial ischemia and anticoagulant. It is worthy of further research and development of the mechanism and clinical application of Vicia faba root in cardiovascular system.
【作者单位】： 上海中医药大学中药研究所·中药标准化教育部重点实验室·中药新资源与质量标准综合评价国家中医药管理局重点研究室·上海中药标准化研究中心;
【基金】：上海市科学技术委员会科研计划项目(No.14401902100)
【分类号】：R29
，
本文编号：2177525
[Abstract]:Objective to observe the pharmacological activities of Vicia faba root in the cardiovascular aspects such as myocardial ischemia and anticoagulant, so as to provide the basis for further exploring the clinical application direction of wild broad bean root and the medicinal value of developing wild broad bean root. Methods the acute toxicity evaluation of Vicia faba root was carried out, and the relative anticoagulant activity of Vicia faba root was investigated by capillary tube method, mouse tail cut method and plasma recalcium test. The protective effect of Vicia faba root on myocardial ischemia injury was studied in mice induced by isoproterenol hydrochloride, and the anti-inflammatory activity of Vicia faba root was observed by carrageenin induced mouse balloon synovitis model. Results the drug of Vicia faba root was safe and could significantly increase the blood coagulation time and bleeding time of mice. The water, methanol and ethyl acetate extracts could also significantly prolong the recalcium time of rabbit plasma in vitro, among which the time of water extraction was the most obvious. It can significantly reduce the contents of serum lactate dehydrogenase and malondialdehyde in mice with acute myocardial ischemia and decrease the exudation of prostaglandin E _ 2 in inflammatory model. Conclusion Vicia faba root has some pharmacological activities in cardiovascular related aspects such as myocardial ischemia and anticoagulant. It is worthy of further research and development of the mechanism and clinical application of Vicia faba root in cardiovascular system.
【作者单位】： 上海中医药大学中药研究所·中药标准化教育部重点实验室·中药新资源与质量标准综合评价国家中医药管理局重点研究室·上海中药标准化研究中心;
【基金】：上海市科学技术委员会科研计划项目(No.14401902100)
【分类号】：R29
，
本文编号：2177525
本文链接：https://www.wllwen.com/zhongyixuelunwen/2177525.html
最近更新
教材专著
