“标本配穴”电针对糖尿病肾病的改善作用及肾脏SIRT1蛋白表达水平的影响

发布时间：2018-08-20 19:18

【摘要】：目的:观察“标本配穴”电针干预方法对糖尿病肾病大鼠模型肾功能、超微结构的改善作用及肾脏SIRT1蛋白表达的影响。方法:Zucker fa/fa大鼠12只按随机数字表法分为模型对照组及非电针刺激组、电针治疗组,另选其同窝瘦鼠(Zucker+/?)4只作为空白对照组。非电针刺激组和电针治疗组分别治疗3周,于治疗后第21天测其4组大鼠的饮水量、进食量、体质量和空腹血糖、餐后血糖;治疗3周后大鼠处死,观察血清白蛋白、血肌酐、血尿素、血尿氮、肾脏超微结构及SIRT1蛋白表达水平。结果:与模型对照组比较,“标本配穴”电针治疗组大鼠进食量减少,体质量降低,空腹血糖、餐后血糖降低,血肌酐、血尿素、血尿氮水平降低,肾脏超微结构得到明显改善,SIRT1蛋白表达增多。结论:“标本配穴”电针能够改善糖尿病肾病肾功能和超微结构,可能与其上调肾脏SIRT1蛋白表达水平有关。
[Abstract]:Aim: to observe the effect of electroacupuncture intervention on renal function, ultrastructure and SIRT1 protein expression in diabetic nephropathy rats. Methods Twelve percent Zucker fa/fa rats were randomly divided into two groups: model control group, non-electroacupuncture stimulation group, electroacupuncture treatment group, and 4 Zucker /? mice as blank control group. The rats in the non-electroacupuncture stimulation group and the electroacupuncture treatment group were treated for 3 weeks respectively. On the 21st day after treatment, the water intake, food intake, body mass, fasting blood glucose and postprandial blood glucose were measured in the four groups, and the rats were killed after 3 weeks of treatment, and the serum albumin and creatinine were observed. Blood urea, blood urine nitrogen, kidney ultrastructure and SIRT1 protein expression level. Results: compared with the model control group, the feeding quantity, body mass, fasting blood glucose, postprandial blood glucose, serum creatinine, blood urea and blood urine nitrogen level of the rats in the "specimen with acupoint" electroacupuncture group were decreased. The expression of SIRT1 protein was significantly improved in renal ultrastructure. Conclusion: electroacupuncture can improve the renal function and ultrastructure of diabetic patients, which may be related to the up-regulation of renal SIRT1 protein expression.
【作者单位】：湖北中医药大学针灸骨伤学院/针灸治未病湖北省协同创新中心;武汉市第九医院;华中科技大学同济医学院附属协和医院中西医结合科;
【分类号】：R245.97

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