电针合募穴调节肠功能与肠运动神经元的关系研究
发布时间：2018-08-23 07:54
【摘要】：目的：基于肠功能异常的便秘动物模型,探究针刺肠的合穴、募穴、合募穴调节肠功能的效应差异,观察电针干预前后肠神经抑制性运动神经元的变化情况,分析电针不同穴位调节肠功能效应差异与肠抑制性运动神经元的关系,为临床合理选取腧穴治疗肠腑病症提供参考。方法：Sprague-Dawley雄性大鼠4周龄110只,随机分为正常组20只,造模组90只,在造模14天期间给予造模组0-4℃冰水灌胃,并观察大鼠粪便的湿重、干重、首粒排便时间与小肠推进功能。造模结束后第15天,挑选造模成功的大鼠随机分为模型组12只,电针干预三组(电针募穴、电针合穴、电针合募穴组各6只),电针强度1.5mA,电针频率2/15HZ,每只大鼠电针干预15min/天,电针期间观察大鼠粪便的湿重、干重、首粒排便时间进行比较分析,于实验周期第22天将正常组、模型组与电针三组大鼠结肠组织取材并固定,以免疫组化测定近端结肠与远端结肠肌间神经丛nNOS阳性反应神经元平均光密度,进行半定量分析。结果：(1)在造模期间,模型组大鼠粪便湿重、干重均减少,粪便湿重在第2,8,12,14天较正常组比较降低(p0.05),粪便干重在第10,12,14天低于正常组(p0.05),首粒排便时间低于正常组(p0.05)。(2)在电针干预期间,电针三组穴位均能够使粪便湿重向正常水平恢复,电针合募穴优于电针募穴与电针合穴(p0.05)。电针三组穴位均有使粪便干重向正常水平恢复的作用。电针三组穴位对首粒排便时间均有改善,电针募穴有使结肠功能向正常水平恢复的趋势,但并不能够使其恢复至正常水平,电针募穴对结肠功能的改善弱于电针合穴与电针合募穴。(3)在近端结肠肌间神经丛,模型组nNOS阳性神经元显著高于正常组(p0.01)。电针干预治疗后,三组穴位电针治疗均能使便秘大鼠近端结肠肌间神经丛异常升高的nNOS明显降低(p0.01),且电针合穴与电针合募穴均能够nNOS恢复到正常水平,而电针募穴没有出现这一现象,且电针募穴与电针合募穴比较差异有统计学意义(p0.01)。(4)在远端结肠肌间神经丛,模型组nNOS明显高于正常组(p0.01),电针三组穴位均能使异常升高的nNOS降低(p0.01),且电针合穴与电针合募穴能够使nNOS恢复至正常水平,但电针募穴并不能使其恢复至正常水平(p0.01),综上说明远端结肠肌间神经丛nNOS在电针合穴、电针合募穴调节便秘大鼠肠功能中的作用更加明显。结论：(1)本实验再次验证了冰水灌胃复制便秘模型大鼠的可行性与优点,即该造模方法能够使得整个实验周期简短,更易于操作。近、远端结肠肌间神经丛抑制性神经元nNOS的异常升高是导致便秘发生的局部神经机制之一。(2)电针合穴、募穴及合募穴均能有效改善肠功能,使肠功能向正常水平恢复。本研究显示在便秘状态下,两集元穴的配穴作用大于单穴的效应。电针合募穴对便秘大鼠结肠运动的促进作用,可能骶副交感神经更容易被激活从而产生更优的效应,才会使得两集元穴的配伍效应加强。(3)电针募穴调节近、远端结肠肌间神经丛抑制性神经元nNOS的作用较弱,电针合募穴的作用最优。电针合募穴调节肠功能的效应存在差异,近、远端神经肌间神经丛神经元nNOS的的不同改变可能是基础。
[Abstract]:Objective: Based on the constipation animal model of intestinal dysfunction, to explore the difference of regulating intestinal function by acupuncture at Hexue, Muxu and HeMu points, to observe the changes of intestinal nerve inhibitory motor neurons before and after electroacupuncture intervention, and to analyze the relationship between the difference of regulating intestinal function by Electroacupuncture at different acupoints and intestinal inhibitory motor neurons. Methods: 110 Sprague-Dawley male rats aged 4 weeks were randomly divided into the normal group (20 rats) and the model group (90 rats). During the 14 days of modeling, the rats were given ice water at 0-4, and the wet weight, dry weight, first defecation time and small intestinal propulsion function were observed. Twelve rats were randomly divided into three groups: electro-acupuncture group, electro-acupuncture group, electro-acupuncture group and electro-acupuncture group. The intensity of electro-acupuncture was 1.5mA and the frequency of electro-acupuncture was 2/15HZ. Each rat was intervened by electro-acupuncture for 15 min/day. The average optical density of nNOS-positive neurons in the proximal and distal colonic myenteric plexus was determined by immunohistochemistry and semi-quantitative analysis was made. Results: (1) During the modeling period, the wet and dry weight of feces in the model group decreased, and the wet weight of feces in the proximal and distal colonic myenteric plexus decreased. The dry weight of feces on the 10th, 12th and 14th day was lower than that of the normal group (p0.05), and the first defecation time was lower than that of the normal group (p0.05). Electro-acupuncture at three acupoints can improve the time of first bowel movement. Electro-acupuncture at the acupoints has a tendency to restore colonic function to normal level, but can not restore it to normal level. The improvement of colonic function by electro-acupuncture at the acupoints is weaker than that by electro-acupuncture at the acupoints and electro-acupuncture at the acupoints. The nNOS positive neurons in the nerve plexus and the model group were significantly higher than those in the normal group (p0.01). After electroacupuncture treatment, the abnormal increase of nNOS in the proximal colonic myenteric plexus in the constipated rats was significantly decreased by Electroacupuncture at all three acupoints (p0.01), and both electroacupuncture and electroacupuncture at the same acupoint could restore the nNOS to the normal level, but electroacupuncture at the same acupoint did not appear. (4) in the distal colonic myenteric plexus, the nNOS in the model group was significantly higher than that in the normal group (p0.01). electroacupuncture at all three acupoints could reduce the abnormally elevated nNOS (p0.01), and electroacupuncture at both acupoints could restore nNOS to normal level, but electroacupuncture at all three acupoints could restore nNOS to normal level. In conclusion: (1) This experiment once again verified the feasibility and advantages of cold water gastric perfusion in duplicating constipation model rats, that is, this method can make the model. Nearly, the abnormal elevation of nNOS in the distal colonic myenteric plexus inhibitory neurons is one of the local neural mechanisms leading to constipation. (2) Electroacupuncture at Hexue, Muxu and HeMu can effectively improve intestinal function and restore intestinal function to normal level. The effect of acupoints matching is greater than that of single acupoint. The promoting effect of electroacupuncture combined with Mu acupoint on colonic motility in constipated rats may be that the sacral parasympathetic nerve is more easily activated to produce a better effect, so that the compatibility effect of the two Jiyuan acupoints can be strengthened. (3) The effect of electroacupuncture combined with Mu acupoint matching on the inhibition neuron nNOS of distal colonic myenteric plexus near to Electroacupuncture combined with Mu acupoint has different effects on regulating intestinal function, and the different changes of nNOS in distal neuromuscular plexus neurons may be the basis.
【学位授予单位】：南京中医药大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：R245
本文编号：2198382
[Abstract]:Objective: Based on the constipation animal model of intestinal dysfunction, to explore the difference of regulating intestinal function by acupuncture at Hexue, Muxu and HeMu points, to observe the changes of intestinal nerve inhibitory motor neurons before and after electroacupuncture intervention, and to analyze the relationship between the difference of regulating intestinal function by Electroacupuncture at different acupoints and intestinal inhibitory motor neurons. Methods: 110 Sprague-Dawley male rats aged 4 weeks were randomly divided into the normal group (20 rats) and the model group (90 rats). During the 14 days of modeling, the rats were given ice water at 0-4, and the wet weight, dry weight, first defecation time and small intestinal propulsion function were observed. Twelve rats were randomly divided into three groups: electro-acupuncture group, electro-acupuncture group, electro-acupuncture group and electro-acupuncture group. The intensity of electro-acupuncture was 1.5mA and the frequency of electro-acupuncture was 2/15HZ. Each rat was intervened by electro-acupuncture for 15 min/day. The average optical density of nNOS-positive neurons in the proximal and distal colonic myenteric plexus was determined by immunohistochemistry and semi-quantitative analysis was made. Results: (1) During the modeling period, the wet and dry weight of feces in the model group decreased, and the wet weight of feces in the proximal and distal colonic myenteric plexus decreased. The dry weight of feces on the 10th, 12th and 14th day was lower than that of the normal group (p0.05), and the first defecation time was lower than that of the normal group (p0.05). Electro-acupuncture at three acupoints can improve the time of first bowel movement. Electro-acupuncture at the acupoints has a tendency to restore colonic function to normal level, but can not restore it to normal level. The improvement of colonic function by electro-acupuncture at the acupoints is weaker than that by electro-acupuncture at the acupoints and electro-acupuncture at the acupoints. The nNOS positive neurons in the nerve plexus and the model group were significantly higher than those in the normal group (p0.01). After electroacupuncture treatment, the abnormal increase of nNOS in the proximal colonic myenteric plexus in the constipated rats was significantly decreased by Electroacupuncture at all three acupoints (p0.01), and both electroacupuncture and electroacupuncture at the same acupoint could restore the nNOS to the normal level, but electroacupuncture at the same acupoint did not appear. (4) in the distal colonic myenteric plexus, the nNOS in the model group was significantly higher than that in the normal group (p0.01). electroacupuncture at all three acupoints could reduce the abnormally elevated nNOS (p0.01), and electroacupuncture at both acupoints could restore nNOS to normal level, but electroacupuncture at all three acupoints could restore nNOS to normal level. In conclusion: (1) This experiment once again verified the feasibility and advantages of cold water gastric perfusion in duplicating constipation model rats, that is, this method can make the model. Nearly, the abnormal elevation of nNOS in the distal colonic myenteric plexus inhibitory neurons is one of the local neural mechanisms leading to constipation. (2) Electroacupuncture at Hexue, Muxu and HeMu can effectively improve intestinal function and restore intestinal function to normal level. The effect of acupoints matching is greater than that of single acupoint. The promoting effect of electroacupuncture combined with Mu acupoint on colonic motility in constipated rats may be that the sacral parasympathetic nerve is more easily activated to produce a better effect, so that the compatibility effect of the two Jiyuan acupoints can be strengthened. (3) The effect of electroacupuncture combined with Mu acupoint matching on the inhibition neuron nNOS of distal colonic myenteric plexus near to Electroacupuncture combined with Mu acupoint has different effects on regulating intestinal function, and the different changes of nNOS in distal neuromuscular plexus neurons may be the basis.
【学位授予单位】：南京中医药大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：R245
【参考文献】
相关期刊论文 前1条
1 Gabrio Bassotti;Vincenzo Villanacci;;Slow transit constipation: A functional disorder becomes an enteric neuropathy[J];World Journal of Gastroenterology;2006年29期
，本文编号：2198382
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