藏药“蒂达”几种药用植物指纹图谱的建立及其抗肝损伤有效性评价

发布时间：2018-09-14 10:07

【摘要】：目的建立并比较藏药“蒂达”11种基原植物的高效液相色谱指纹图谱,比较藏药“蒂达”4种基原植物(青叶胆、唐古特虎耳草、印度獐牙菜和川西獐牙菜)体内、体外抗肝损伤药效学作用,为临床用药提供科学依据。方法使用HPLC法建立11种“蒂达”基原植物指纹图谱。“蒂达”4种基原植物对小鼠急性肝损伤保护作用研究中,采用CCl4来建立化学性肝损伤模型,以青叶胆、唐古特虎耳草、印度獐牙菜和川西獐牙菜两个剂量(3 g/kg、6 g/kg)灌胃给药,1次/d,连续7d,检测丙氨酸转氨酶(ALT)、谷草转氨酶(AST)及总蛋白(TP),计算肝脏指数及观察肝脏病理组织切片。对小鼠慢性肝损伤保护作用研究中,小鼠皮下注射20%CCl4(2次/周),以青叶胆、唐古特虎耳草、印度獐牙菜和川西獐牙菜两个剂量(1.5 g/kg、3 g/kg)灌胃给药。1次/d,连续45天,检测丙氨酸转氨酶(ALT)、谷草转氨酶(AST)、总蛋白(TP)及总胆红素(TBI),计算肝脏指数、脾脏指数及胰腺指数和观察肝脏病理组织切片。体外抗肝损伤研究中,测定细胞培养上清液中谷丙转氨酶(ALT)、谷草转氨酶(AST)、丙二醛(MDA)含量和超氧化物歧化酶(SOD)活力。结果本课题完成了11种“蒂达”基原植物指纹图谱共有模式的建立及相似度评价。“蒂达”四种基原植物对小鼠急性肝损伤保护作用研究中,4种药物都能够显著降低ALT、AST含量(P0.01),川西獐牙菜6 g/kg组效果最好。对小鼠慢性肝损保护作用研究中,4种药物都能够显著降低ALT、AST含量(P0.01),改善肝脏组织病理损伤。川西獐牙菜、印度獐牙菜的保肝效果优于青叶胆。体外抗肝损伤研究中,给药组与模型组比较ALT、AST、MDA含量均下降,SOD活力提升。4种原植物在不同浓度时对不同的酶的作用效果不同,存在一定的差异,综合结果显示:川西獐牙菜、印度獐牙菜的保肝效果优于青叶胆。结论本课题建立了较完整的藏药“蒂达”的高效液相色谱指纹图谱,该方法为其质量控制及评价提供科学依据。青叶胆、唐古特虎耳草、川西獐牙菜和印度獐牙菜均对肝脏有一定保护作用,不同种不同浓度作用于急、慢性CCl4损伤小鼠的抗肝损伤作用存在一定差异,不同种不同浓度对人肝细胞L-O2中不同酶作用效果不同,其机制可能与抗氧化有关。本课题为指导临床用药提供依据。
[Abstract]:OBJECTIVE To establish and compare the high performance liquid chromatographic fingerprints of 11 primordial plants of the Tibetan medicine Tida, and to compare the pharmacodynamic effects of four primordial plants of the Tibetan medicine Tida in vitro and in vivo, so as to provide scientific basis for clinical use. In the study of the protective effect of the four original plants on acute liver injury in mice, CCl4 was used to establish the model of chemical liver injury. Two doses (3 g/kg, 6 g/kg) were given orally, once a day, for 7 days, to detect alanine aminotransferase. To study the protective effects of 20% CCl4 (twice a week) subcutaneously on chronic liver injury in mice, two doses (1.5 g/kg, 3 g/kg) were administered orally to the mice, i.e. green leaf gall, Tangut tiger's ear grass, Swertia glauca and Swertia roxburghii. Alanine aminotransferase (ALT), glutamic oxaloacetic aminotransferase (AST), total protein (TP) and total bilirubin (TBI), liver index, spleen index and pancreatic index were calculated, and liver pathological sections were observed. Alanine aminotransferase (ALT), glutamic oxaloacetic aminotransferase (AST), malondialdehyde (MDA) content and malondialdehyde (MDA) content in cell culture supernatant were measured in vitro. Superoxide dismutase (SOD) activity. Results The establishment and similarity evaluation of the fingerprint patterns of 11 Tida primordial plants were completed in this study. Good. In the study of chronic liver injury in mice, the four drugs can significantly reduce the content of ALT, AST (P 0.01), improve liver tissue pathological injury. Swertia, Swertia Swertia, liver protection effect is better than that of Cyanobacteria. In the study of anti-liver injury in vitro, compared with the model group, the content of ALT, AST, MDA decreased, SOD activity increased. The results showed that the liver-protecting effect of Swertia roxburghii and Swertia roxburghii was better than that of Cyanopsis indica. Conclusion A complete high performance liquid chromatography fingerprint of Tida was established, which provided a basis for quality control and evaluation. Evidence. Cyclobalanopsis, Tanggut Saxifraga, Swertia Swertia and Swertia Swertia have protective effects on the liver. Different concentrations have different effects on acute and chronic CCl4-induced liver injury in mice. Different concentrations have different effects on different enzymes in human hepatocyte L-O2. The mechanism may be related to antioxidant effect. This topic provides a basis for guiding clinical medication.
【学位授予单位】：大理大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R29

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