热补针法对类风湿关节炎寒证模型家兔血液代谢物谱的影响

发布时间：2018-10-05 08:44

【摘要】：目的:利用液质联用(LC-MS)技术研究热补针法对类风湿关节炎(RA)模型家兔血液代谢物谱的影响,探讨热补针法治疗RA的特异性机制。方法:40只清洁级青紫蓝家兔随机分为正常组、模型组、平补平泻组、捻转补法组和热补针法组,每组8只。除正常组外,余组以卵蛋白诱导法联合低温冷冻法复制RA寒证模型。正常组、模型组不予针刺干预,平补平泻组、捻转补法组和热补针法组于"足三里"分别施以平补平泻、捻转补法、热补针法针刺,留针30 min,每日1次,共7 d。干预结束后测定各组痛阈值以及膝关节表面温度;心脏采血制备血清,对血清进行代谢组学检测;并对数据进行主成分分析(PCA)和偏最小二乘法-判别分析(PLS-DA),通过变量重要性投影值(VIP1)和P值(P0.05)筛选出血清中的差异代谢物。结果:与正常组相比,模型组家兔痛阈、膝关节表面温度降低(均P0.05);与模型组比较,各针刺组干预后痛阈、膝关节表面温度升高(均P0.05);热补针法组优于平补平泻组、捻转补法组(均P0.05)。与正常组相比,模型组血液代谢物中肉碱、Lyso PC(14∶0)、Lyso PC(18∶3)、Lyso PE(0∶0/20∶5)、Lyso PE(0∶0/22∶1)、癸酸、硬脂酸以及乳酸的含量均升高,亮氨酸、缬氨酸、谷氨酰胺、焦谷氨酸、α-酮戊二酸、琥珀酸、延胡索酸、苹果酸、半乳糖、甘露糖含量均降低,上述代谢物具体涉及到脂肪酸、氨基酸、三羧酸循环以及糖类代谢。各针刺组干预后上述代谢物均出现不同程度的回调。热补针法组较平补平泻组和捻转补法组乳酸的含量降低,琥珀酸、延胡索酸、苹果酸、半乳糖、甘露糖的含量升高。结论:热补针法治疗RA的特异性体现在对三羧酸循环与糖代谢的调控。
[Abstract]:Objective: to study the effect of hot acupuncture on the blood metabolites spectrum of rabbits with rheumatoid arthritis (RA) by using liquid / mass spectrometry (LC-MS), and to explore the specific mechanism of heat supplement acupuncture in the treatment of RA. Methods 40 clean blue rabbits were randomly divided into normal group, model group, Pingbuping diarrhea group, twirling supplement group and hot reinforcing acupuncture group with 8 rabbits in each group. In addition to normal group, RA cold syndrome model was induced by ovalbumin induction combined with cryopreservation. Normal group, model group without acupuncture intervention, Pingbu Pingxie group, twirling reinforcing method group and heat reinforcing acupuncture group were treated with "Zusanli" in "Zusanli", twisting reinforcing method, heat reinforcing acupuncture method, retention needle 30 min, daily for 7 days. After the intervention, the pain threshold and the surface temperature of knee joint were measured. The data were analyzed by principal component analysis (PCA) and partial least squares discriminant analysis (PLS-DA). The differential metabolites in serum were screened by variable importance projection value (VIP1) and P value (P0.05). Results: compared with the normal group, the pain threshold and knee joint surface temperature in the model group decreased (P0.05), compared with the model group, the pain threshold and the knee joint surface temperature increased after acupuncture intervention (P0.05). Twirling group (P0.05). Compared with the normal group, the contents of carnitine PC (14:0) and PC (18:3) in the blood metabolites of the model group were higher than those in the normal group (0: 0 / 20: 5) PE (0: 0 / 22: 1), acid, sebacic acid and lactic acid, leucine, valine, glutamine, pyroglutamic acid, 伪 -ketoglutaric acid, leucine, valine, glutamine, pyroglutamic acid, 伪 -ketoglutaric acid, The contents of succinic acid, fumaric acid, malic acid, galactose and mannose were all decreased. These metabolites were related to fatty acid, amino acid, tricarboxylic acid cycle and carbohydrate metabolism. After the intervention of each acupuncture group, the above metabolites showed a different degree of callback. The content of lactic acid in heat supplement acupuncture group was lower than that in Pingbuping diarrhea group and twist reinforcing method group, and the contents of succinic acid, fumaric acid, malic acid, galactose and mannose were increased. Conclusion: the regulation of tricarboxylic acid cycle and glucose metabolism is the specificity of heat-reinforcing acupuncture in the treatment of RA.
【作者单位】：甘肃中医药大学针灸推拿学院;兰州大学第二医院中医科;甘肃省中医院针灸科;
【基金】：国家自然科学基金项目:81260558 兰州市科技计划项目:2014-1-247 国家中医药管理局甘肃郑氏针法学术流派传承工作室项目:2305135901
【分类号】：R245;R-332

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