艾灸听宫对大鼠膜迷路积水的影响

发布时间：2018-10-07 17:33

【摘要】：目的:观察艾灸听宫对膜迷路积水模型大鼠耳蜗形态、血浆血管加压素(arginine vasopressin,AVP)浓度及耳蜗水通道蛋白2(aquaporin2,AQP2)表达的影响,探讨艾灸听宫对膜迷路积水的可能调节机制。方法:将28只Wistar大鼠随机分为空白对照组,模型对照组,托伐普坦组及艾灸听宫组,每组7只。除外空白对照组,其余各组均采用腹腔注射AVP的方法复制膜迷路积水模型。空白对照组与模型对照组采取与艾灸听宫组相同的固定,不予治疗;托伐普坦组予托伐普坦溶液5 mg/kg灌胃;艾灸听宫组于听宫穴施灸20 min。各治疗组均每日治疗1次,连续治疗10 d。干预结束后,行HE染色法观察大鼠耳蜗积水程度,计算蜗管横截面积与蜗管加前庭阶横截面积之和的比值(R值);放免法检测血浆AVP浓度;免疫组化法检测耳蜗血管纹AQP2蛋白的表达水平。结果:(1)模型对照组出现膜迷路积水,前庭膜向前庭阶方向不同程度的隆起扩张,R值较空白对照组增加(P0.01);托伐普坦组与艾灸听宫组前庭膜隆起程度均较模型对照组不同程度的减轻,R值均较模型对照组降低(P0.01,P0.05);艾灸听宫组R值与托伐普坦组比较,差异无统计学意义(P0.05)。(2)模型对照组大鼠血浆AVP浓度较空白对照组升高(P0.01);托伐普坦组与艾灸听宫组血浆AVP浓度均较模型对照组降低(均P0.05);艾灸听宫组血浆AVP浓度与托伐普坦组比较,差异无统计学意义(P0.05)。(3)模型对照组耳蜗血管纹AQP2表达水平较空白对照组升高(P0.01);托伐普坦组与艾灸听宫组AQP2表达水平均较模型对照组降低(均P0.01);艾灸听宫组AQP2表达水平与托伐普坦组比较,差异无统计学意义(P0.05)。结论:艾灸听宫可减轻大鼠膜迷路积水,其作用机制可能与对AVP-AQP2系统的调节有关。
[Abstract]:Objective: to observe the effects of moxibustion on cochlea morphology, plasma vasopressin (arginine vasopressin,AVP) concentration and cochlear aquaporin-2 (aquaporin2,AQP2) expression in rats with membranous labyrinth hydrops, and to explore the possible regulatory mechanism of moxibustion on membranous labyrinthine hydrops. Methods: Twenty-eight Wistar rats were randomly divided into three groups: blank control group, model control group, Tovaptan group and moxibustion auricular group with 7 rats in each group. Except the blank control group, the other groups were treated by intraperitoneal injection of AVP to establish the model of hydrolabyrinth. The blank control group and the model control group were treated with the same fixation as the moxibustion group without treatment; the torphaptan group was given tovastam solution for 5 mg/kg; the moxibustion group was treated with moxibustion at the hearing point for 20 min. All the treatment groups were treated once a day for 10 days. After the intervention, the degree of cochlea hydrops in rats was observed by HE staining, the ratio of cochlear duct cross-sectional area to cochlear duct plus vestibular cross sectional area (R value) was calculated, the plasma AVP concentration was detected by radioimmunoassay. The expression of AQP2 protein in stria vascularis of cochlea was detected by immunohistochemical method. Results: (1) the membrane labyrinth hydrops appeared in the model control group. The R value of vestibular membrane to vestibular step was higher than that of blank control group (P0.01), and the vestibular membrane bulge degree of Tofaptan group and moxibustion auricular temple group was lower than that of model control group (P0.01), and the R value of vestibular membrane bulge in Tofaptan group and moxibustion aegong group was lower than that in model control group. The R value of moxibustion group was higher than that of torphoptan group, while that of moxibustion group was lower than that of Tofaptan group. There was no significant difference (P0.05). (2). The plasma AVP concentration in the model group was higher than that in the blank control group (P0.01); the plasma AVP concentration in the Tofaptan group and moxibustion auricular group was lower than that in the model control group (P0.05); the plasma AVP concentration in the moxibustion hearing palace group was higher than that in the Topaptan group. There was no significant difference (P0.05). (3) in the expression of AQP2 in the cochlear stria of the model control group compared with the blank control group (P0.01), the level of AQP2 expression in the Tofaptan group and the moxibustion auricular group was lower than that in the model control group (P0.01), and the expression of AQP2 in the moxibustion group was lower than that in the model control group (P0.01). Compared with the Tofaptan formation, The difference was not statistically significant (P0.05). Conclusion: moxibustion can relieve the membranous labyrinthine hydrops in rats, and its mechanism may be related to the regulation of AVP-AQP2 system.
【作者单位】：浙江中医药大学附属广兴医院;浙江中医药大学;
【基金】：国家自然科学基金项目(81373757)
【分类号】：R245.81

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