秩边透水道技术对原发性痛经模型大鼠T淋巴细胞亚群和前列腺素的影响
发布时间：2018-10-10 06:36
【摘要】：目的:1、观察分析秩边透水道技术治疗原发性痛经模型大鼠的疗效及其镇痛作用,并与现代常用针刺方法相比较,观察两种治疗方法的疗效差别。为针灸治疗原发性痛经提供多元化的治疗方法。2、观察秩边透水道技术对原发性痛经模型大鼠外周血T淋巴细胞亚群CD3~+、CD4~+的百分数及CD4/CD8比值、对大鼠子宫内膜前列腺素E2和F2α的含量、对痛经模型大鼠胸腺、脾脏的病理变化的影响,从免疫学角度,探讨秩边透水道技术对原发性痛经的特异性治疗作用,为临床应用和推广提供可靠的实验数据和科学依据,从而更好的指导临床。方法:将40只健康清洁级雌性未孕SD大鼠随机分为4组,每组十只。分为空白对照组、模型组、针刺组、秩边透水道组。具体操作方法遵从文献方法并加以改进,四组同期进行。首先在模型组,针刺组,秩边透水道组大鼠股部皮下注射苯甲酸雌二醇10天,每日1次,第1天0.5mg/只,第2-9天0.2mg/只,第10天0.5mg/只,第11天腹腔注射催产素2u/只。对照组大鼠股部皮下注射同等计量的生理盐水10天,每日1次,第11天腹腔注射生理盐水0.2ml/只。观察记录腹腔注射药物后30分钟内大鼠扭体反应发生的次数。采用流式细胞仪检测法观察各组大鼠外周血T淋巴细胞亚群CD3~+、CD4~+、CD8~+的百分数,采用放射免疫法检测各组大鼠子宫内膜前列腺素E2及F2α的含量,采用HE染色法观察各组大鼠胸腺、脾脏的病理变化。结果:1、腹腔注射药物后30min内,空白对照组没有出现扭体反应,其余三组则均出现不同程度的扭体反应,以模型组扭体次数最多,与空白对照组比较,差异有统计学意义(P0.05),说明本次实验造模成功。针刺组和秩边透水道组扭体次数明显减少,与模型组比较有显著差异,且差异有统计学意义(P0.05)。说明此两组都能够有效的治疗原发性痛经,减轻扭体次数的发生。2、与空白对照组比较,模型组大鼠子宫内膜PGF2α含量明显升高,PGE含量明显降低,PGF2α/PGE2比值明显升高,差异有统计学意义(P0.05),从病因角度说明造模成功;针刺组、秩边透水道组与模型组相比较,PGF2α含量明显降低,PGE2含量明显升高,PGF2α/PGE2比值降低,差异有统计学意义(P0.05),说明针刺组和秩边透水道组对原发性痛经有镇痛作用。3、与空白对照组比较,模型组CD3~+、CD4~+、CD8~+水平均出现明显变化,CD3~+、CD4~+水平以及CD4/CD8水平降低,差异有统计学意义(P0.05),针刺组、秩边透水道组与模型组相比,CD3~+、CD4~+及CD4/CD8水平上升,趋向于空白对照组T淋巴细胞亚群水平,差异有统计学意义(P0.05)。说明针刺组和秩边透水道组都能够调节痛经模型大鼠的免疫功能。4、针刺组和秩边透水道组各个指标的比较差异均无统计学意义(P0.05),说明此次实验不能有效的证明传统针刺法与秩边透水道法治疗效果的优劣。5、胸腺、脾脏在病理变化方面,模型组均发生了明显的病理变化,而针刺组和秩边透水道组的胸腺、脾脏的病理变化程度较模型组轻微。从病理学角度说明了秩边透水道技术对原发性痛经免疫系统的影响。结论:1、秩边透水道技术对原发性痛经有镇痛作用。2、秩边透水道技术在一定程度上能够调节痛经模型大鼠的免疫功能。
[Abstract]:Objective: 1. To observe the curative effect and its analgesic effect in the treatment of primary dysmenorrhea model rats. To provide a method for the treatment of primary dysmenorrhea by acupuncture and moxibustion. 2. The percentage of CD3 +, CD4 + and CD4/ CD8 in peripheral blood T lymphocytes in the peripheral blood T lymphocytes of primary dysmenorrhea model rats were studied by using the technique of rank-edge pervious channel. The contents of prostaglandin E2 and F2 in the endometrium of rats were determined. In order to provide reliable experimental data and scientific basis for clinical application and promotion, we can better guide the clinical application. Methods: Forty healthy clean-grade female non-pregnant SD rats were randomly divided into 4 groups, each group was 10 rats. It is divided into blank control group, model group, acupuncture group, rank-edge pervious channel group. The specific operation method complies with the literature method and improves it, and the four groups are carried out in the same period. In the model group, the acupuncture group and the rat femoral component were injected with estradiol benzoate 10 days, once daily, day 1 0. 5mg/ day, day 2-9 day 0. 2mg/ day, 10th day 0. 5mg/ day, and oxytocin 2u/ day was injected intraperitoneally on day 11. Rats in the control group were injected with normal saline for 10 days at the same time, once every day, and normal saline was injected into the abdominal cavity at day 11 for 0. 2ml/ day. The number of torsion body reactions in rats after intraperitoneal injection was observed for 30 minutes. The percentages of CD3 ~ +, CD4 ~ + and CD8 ~ + in peripheral blood T lymphocytes in each group were observed by flow cytometry. The contents of prostaglandin E2 and F2 in the endometrium of rats were detected by radioimmunoassay. The thymus of each group was observed by HE staining. Pathological changes of spleen. Results: 1. In 30 minutes after intraperitoneal injection, no torsion reaction occurred in the blank control group. The other three groups showed a different degree of torsion body response. The number of torsion bodies in the model group was the largest, and the difference was statistically significant compared with the blank control group (P0.05). There was a significant difference in the number of torsion bodies in the acupuncture group and the group with the rank side, and the difference was statistically significant (P0.05). 2. Compared with the blank control group, the content of PGF2 in the endometrium of the model group was obviously increased, the content of NPY was obviously decreased, and the ratio of PF2 and PF2 was significantly higher than that in the blank control group (P <0.05). Compared with the model group, the content of PGF2 was significantly lower than that in the model group (P <0.05). It was indicated that the acupuncture group and the rank-side water-permeable waterway group had an analgesic effect on primary dysmenorrhea. Compared with the blank control group, the levels of CD3 +, CD4 + and CD8 ~ + in the model group were significantly changed, and the levels of CD3 +, CD4 + and CD4/ CD8 were decreased, and the difference was statistically significant (P0.05). Compared with the model group, the level of CD3 +, CD4 ~ + and CD4/ CD8 increased in the acupuncture group, and the difference was statistically significant (P <0.05). The results showed that both the acupuncture group and the rank-side water-permeable waterway group were able to adjust the immune function of the rats with dysmenorrhea model. The pathological changes of the thymus and spleen were observed in the model group. The pathological changes of the spleen were lower than that in the model group. From the pathological point of view, the influence of the technique on the immune system of primary dysmenorrhea was described. Conclusion: 1. The technique of rank-edge water-permeable waterway has analgesic effect on primary dysmenorrhea. The technique of rank-edge water-permeable waterway can regulate the immune function of menorrheal model rats to some extent.
【学位授予单位】：山西中医学院
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：R245
[Abstract]:Objective: 1. To observe the curative effect and its analgesic effect in the treatment of primary dysmenorrhea model rats. To provide a method for the treatment of primary dysmenorrhea by acupuncture and moxibustion. 2. The percentage of CD3 +, CD4 + and CD4/ CD8 in peripheral blood T lymphocytes in the peripheral blood T lymphocytes of primary dysmenorrhea model rats were studied by using the technique of rank-edge pervious channel. The contents of prostaglandin E2 and F2 in the endometrium of rats were determined. In order to provide reliable experimental data and scientific basis for clinical application and promotion, we can better guide the clinical application. Methods: Forty healthy clean-grade female non-pregnant SD rats were randomly divided into 4 groups, each group was 10 rats. It is divided into blank control group, model group, acupuncture group, rank-edge pervious channel group. The specific operation method complies with the literature method and improves it, and the four groups are carried out in the same period. In the model group, the acupuncture group and the rat femoral component were injected with estradiol benzoate 10 days, once daily, day 1 0. 5mg/ day, day 2-9 day 0. 2mg/ day, 10th day 0. 5mg/ day, and oxytocin 2u/ day was injected intraperitoneally on day 11. Rats in the control group were injected with normal saline for 10 days at the same time, once every day, and normal saline was injected into the abdominal cavity at day 11 for 0. 2ml/ day. The number of torsion body reactions in rats after intraperitoneal injection was observed for 30 minutes. The percentages of CD3 ~ +, CD4 ~ + and CD8 ~ + in peripheral blood T lymphocytes in each group were observed by flow cytometry. The contents of prostaglandin E2 and F2 in the endometrium of rats were detected by radioimmunoassay. The thymus of each group was observed by HE staining. Pathological changes of spleen. Results: 1. In 30 minutes after intraperitoneal injection, no torsion reaction occurred in the blank control group. The other three groups showed a different degree of torsion body response. The number of torsion bodies in the model group was the largest, and the difference was statistically significant compared with the blank control group (P0.05). There was a significant difference in the number of torsion bodies in the acupuncture group and the group with the rank side, and the difference was statistically significant (P0.05). 2. Compared with the blank control group, the content of PGF2 in the endometrium of the model group was obviously increased, the content of NPY was obviously decreased, and the ratio of PF2 and PF2 was significantly higher than that in the blank control group (P <0.05). Compared with the model group, the content of PGF2 was significantly lower than that in the model group (P <0.05). It was indicated that the acupuncture group and the rank-side water-permeable waterway group had an analgesic effect on primary dysmenorrhea. Compared with the blank control group, the levels of CD3 +, CD4 + and CD8 ~ + in the model group were significantly changed, and the levels of CD3 +, CD4 + and CD4/ CD8 were decreased, and the difference was statistically significant (P0.05). Compared with the model group, the level of CD3 +, CD4 ~ + and CD4/ CD8 increased in the acupuncture group, and the difference was statistically significant (P <0.05). The results showed that both the acupuncture group and the rank-side water-permeable waterway group were able to adjust the immune function of the rats with dysmenorrhea model. The pathological changes of the thymus and spleen were observed in the model group. The pathological changes of the spleen were lower than that in the model group. From the pathological point of view, the influence of the technique on the immune system of primary dysmenorrhea was described. Conclusion: 1. The technique of rank-edge water-permeable waterway has analgesic effect on primary dysmenorrhea. The technique of rank-edge water-permeable waterway can regulate the immune function of menorrheal model rats to some extent.
【学位授予单位】：山西中医学院
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：R245
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