瑶药小钻乙酸乙酯提取物抗类风湿关节炎作用及相关机制探讨

发布时间：2018-10-12 12:08

【摘要】：目的探讨瑶药小钻乙酸乙酯提取物抗类风湿关节炎(rheumatoid arthritis,RA)作用及相关机制。方法取雄性SD大鼠70只,随机分为正常对照组、模型对照组、雷公藤组、地塞米松组、小钻乙酸乙酯提取物低、中、高剂量组。建立胶原性关节炎(collagen induced arthritis,CIA)大鼠模型,连续灌胃给药28 d,观察大鼠状态、体重、足肿胀度、关节炎指数、踝关节病变;采用ELISA法测定血清白细胞介素-1β(IL-1β)、白细胞介素-6(IL-6)及肿瘤坏死因子-α(TNF-α)含量;采用实时荧光定量PCR法检测关节滑膜组织NF-κB p65 mRNA表达。结果小钻乙酸乙酯提取物能显著抑制CIA大鼠足肿胀,降低血清IL-1β、IL-6、TNF-α及滑膜组织NF-κB p65 mRNA水平,作用强于雷公藤,弱于地塞米松,与剂量相关。结论小钻有一定抗RA作用,其机制可能与NF-κB p65转录有关。
[Abstract]:Objective to investigate the anti-rheumatoid arthritis (rheumatoid arthritis,RA) effect of ethyl acetate extract from Chinese herbal medicine. Methods 70 male SD rats were randomly divided into normal control group, model control group, Tripterygium wilfordii group, dexamethasone group and low, middle and high dose groups. The rat model of collagen-induced arthritis (collagen induced arthritis,CIA) was established. The rats were administrated intragastrically for 28 days. The state, body weight, foot swelling, arthritis index and ankle lesion were observed. The levels of serum interleukin-1 尾 (IL-1 尾), interleukin-6 (IL-6) and tumor necrosis factor- 伪 (TNF- 伪) were measured by ELISA assay, and the expression of NF- 魏 B p65 mRNA in synovial tissue was detected by real-time fluorescence quantitative PCR method. Results the ethyl acetate extract significantly inhibited foot swelling in CIA rats, and decreased the levels of serum IL-1 尾, IL-6,TNF- 伪 and NF- 魏 B p65 mRNA in synovial tissue, which was stronger than Tripterygium wilfordii and weaker than that of dexamethasone in dose-dependent manner. Conclusion small drill has some anti-RA effect, and its mechanism may be related to the transcription of NF- 魏 B p65.
【作者单位】：广西卫生职业技术学院生理教研室;广西药用植物研究所广西药用资源保护与遗传改良重点实验室;
【基金】：国家自然科学基金资助项目(81660721) 广西教育厅高校科研资助项目(KY2015YB461) 广西卫计委自筹经费资助项目(Z2015442)
【分类号】：R29

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