电针对功能性消化不良大鼠GAS和SS的影响
发布时间：2018-10-21 09:11
【摘要】：目的以功能性消化不良(FD)大鼠为研究对象,观察电针对功能性消化不良模型大鼠一般情况,检测尿D-木糖排泄率、胃蛋白酶含量及胃排空率和小肠推进率,探讨脾虚气滞证候模型大鼠的制备,及电针该模型大鼠胃肠消化功能的影响;检测血清和胃组织中胃泌素(GAS)、生长抑素(SS)的表达,探讨GAS和SS在电针调节胃肠功能中发挥的作用。进一步明确电针治疗FD的作用机理。方法选取24只雄性SD大鼠,按照随机数字表法分为空白组、模型组、电针组,每组8只。除空白组外,其余各组均采用夹尾刺激法配合隔日进食制备FD大鼠模型,造模成功后,电针组给予电针“足三里”及“太冲”治疗,空白组、模型组予束缚固定,每日1次,连续治疗14d。实验过程中观测各组大鼠一般情况和尿D-木糖排泄率,治疗结束后,检测胃排空率,小肠推进率及胃蛋白酶含量,苏木素-伊红(HE)染色观察胃窦组织,双抗体夹心酶联免疫吸附测定法(ELISA)测定血清和胃窦中GAS、SS的表达水平。结果1.一般情况观察比较,造模后,与空白组相比,模型组、电针组可见大鼠烦躁、饮水、饮食减少、毛发枯黄无光泽、大便稀溏,两组鼠体重明显轻于空白组,差异具有统计学意义(p0.05)。电针组经治疗后,一般情况较模型组有所改善,大鼠体重较模型组增长明显,差异具有统计学意义(p0.05),且与空白组差异无统计学意义(p0.05)。2.胃排空率比较,与空白组相比,模型组大鼠的胃排空率降低,差异具显著性差异(p0.01);与模型组相比,电针组大鼠胃排空率增加,差异具有统计学意义(p0.05)。3.小肠推进率比较,与空白组相比,模型组大鼠的小肠推进率显著下降,差异具有统计学意义(p0.01);与模型组相比,电针组大鼠的小肠推进率升高,差异具有统计学意义(p0.05)。4.胃蛋白酶含量比较,与空白组相比,模型组大鼠的胃蛋白酶含量减少,差异具有统计学意义(p0.01);与模型组相比,电针组大鼠的胃蛋白酶含量增加,差异具有统计学意义(p0.05)。5.尿D-木糖排泄率比较,与空白组相比,模型组大鼠的尿d-木糖排泄率降低,差异具有统计学意义(p0.05);与模型组相比,电针组大鼠的尿d-木糖排泄率升高,差异具有统计学意义(p0.05)。6.胃窦和血清中GAS和SS含量比较,与空白组相比,模型组大鼠胃窦和血清中gas含量显著降低,ss含量显著升高,差异均具有统计学意义(p0.01);与模型组相比,电针组大鼠胃窦和血清中gas含量升高,差异具有统计学意义(p0.05),ss含量明显降低,差异具有统计学意义(p0.01)。结论1.通过夹尾刺激+隔日进食的方法造模后,大鼠食量减少、体重下降,胃窦解剖肉眼未见器质性病变,说明造模成功。2.电针组体重、胃排空率、小肠推进率、胃蛋白酶含量、尿D-木糖排泄率都增加,说明电针具有治疗作用,可以改善胃肠道功能。3.电针组胃窦和血清中GAS和SS含量变化,与胃排空率、小肠推进率、胃蛋白酶含量、尿D-木糖排泄率的相关性。电针对GAS、SS表达的调节作用可能是电针治疗FD的机制之一。
[Abstract]:Objective To study the general situation of functional dyspepsia (FD) rats, and to detect the excretion rate of urine D-xylose, pepsin content and gastric emptying rate and small intestine propulsion rate in rats with functional dyspepsia. The function of gastrin (GAS), somatostatin (SS) in serum and gastric tissue was detected, and the role of GAS and SS in the regulation of gastrointestinal function was discussed. To further clarify the mechanism of the action of acupuncture on FD. Methods Twenty-four male SD rats were divided into two groups: blank group, model group and EA group. In addition to the blank group, FD rat model was prepared by the combination of tail-and-tail method. After the model was successful, the electro-acupuncture group was given electro-acupuncture 鈥渇ootstool鈥，
本文编号：2284654
[Abstract]:Objective To study the general situation of functional dyspepsia (FD) rats, and to detect the excretion rate of urine D-xylose, pepsin content and gastric emptying rate and small intestine propulsion rate in rats with functional dyspepsia. The function of gastrin (GAS), somatostatin (SS) in serum and gastric tissue was detected, and the role of GAS and SS in the regulation of gastrointestinal function was discussed. To further clarify the mechanism of the action of acupuncture on FD. Methods Twenty-four male SD rats were divided into two groups: blank group, model group and EA group. In addition to the blank group, FD rat model was prepared by the combination of tail-and-tail method. After the model was successful, the electro-acupuncture group was given electro-acupuncture 鈥渇ootstool鈥，
本文编号：2284654
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