电针对高脂饮食诱导的肥胖小鼠白色脂肪组织中腺苷受体表达的影响

发布时间：2018-10-22 14:41

【摘要】：目的:观察电针对高脂饮食诱导肥胖(DIO)小鼠白色脂肪组织(WAT)中腺苷受体(AR)的影响,探讨针刺减肥的外周作用机制。方法:将40只3周龄雄性C 57BL/6小鼠随机分为普食组(12只)和高脂组(28只),采用高脂饮食复制DIO模型。小鼠分为正常对照组(5只)、正常电针组(7只)、模型对照组(6只)、模型电针组(12只)。两个电针组均电针"后三里"和"内庭"穴,每周治疗6次,共电针4周。测量小鼠体质量,计算双侧附睾脂肪组织(Epi-WAT)脂体比,分别用qPCR、Western blot法检测Epi-WAT中A1R、A_(2A)R、A_(2B)R、A3R的mRNA及蛋白表达。结果:与普食组相比,高脂喂养12周能明显增加小鼠体质量(P0.01),其中,体质量超过普食组20%的小鼠有18只(占64.2%);治疗结束后,与正常对照组相比,模型对照组体质量依然维持在较高状态(P0.05),体质量变化值差异有统计学意义(P0.01),Epi-WAT脂体比升高(P0.05);与模型对照组相比,模型电针组体质量、Epi-WAT脂体比均降低(P0.05),体质量变化值增大(P0.01)。A1R mRNA在4个组中表达的差异无统计学意义(P0.05);与正常对照组相比,模型对照组A3R mRNA的表达显著降低(P0.01),A_(2A)R和A_(2B)R蛋白表达降低(P0.01);与模型对照组相比,模型电针组A_(2A)R和A_(2B)R mRNA及蛋白表达均升高(P0.05,P0.01)。结论:电针可以调控WAT中A_(2A)R、A_(2B)R的表达,这可能是电针通过促进外周WAT代谢从而实现减肥效应的部分机制。
[Abstract]:Aim: to observe the effect of electroacupuncture on adenosine receptor (AR) in white adipose tissue (WAT) of obese (DIO) mice induced by high fat diet, and to explore the mechanism of peripheral action of acupuncture on weight loss. Methods: forty 3-week-old male C 57BL/6 mice were randomly divided into common diet group (n = 12) and hyperlipidemia group (n = 28). DIO model was induced by high-fat diet. Mice were divided into normal control group (n = 5), normal electroacupuncture group (n = 7), model control group (n = 6) and model electroacupuncture group (n = 12). The two electroacupuncture groups were treated 6 times a week for 4 weeks. The body mass of mice was measured and the ratio of lipid to body mass of bilateral epididymal adipose tissue (Epi-WAT) was calculated. The expression of mRNA and protein in Epi-WAT was detected by qPCR,Western blot method. Results: compared with the normal diet group, the body weight of the mice was significantly increased after 12 weeks of high fat feeding (P0.01), in which 18 mice (64.2%) with body mass more than 20% of the normal diet group were compared with the normal control group after the treatment. The body mass of the model control group was still in a higher state (P0.05), the difference of body mass was statistically significant (P0.01), the ratio of Epi-WAT lipid to body mass increased (P0.05); compared with the model control group, The body weight, Epi-WAT lipid / body ratio of the model electroacupuncture group were decreased (P0.05), the body mass change value increased (P0.01). There was no significant difference in A1R mRNA expression in the four groups (P0.05); compared with the normal control group, there was no significant difference in A1R mRNA expression between the four groups (P0.05). The expression of A3R mRNA, A2A R and A2B R protein in the model control group was significantly decreased (P0.01), and the expression of A2A R and A2B) R mRNA and protein in the model electroacupuncture group was higher than that in the model control group (P0.05P 0.01). Conclusion: electroacupuncture can regulate the expression of A2A RGA-A2B R in WAT, which may be the mechanism of electroacupuncture to achieve the effect of weight loss by promoting the metabolism of peripheral WAT.
【作者单位】：南京中医药大学针药结合教育部重点实验室;
【基金】：国家自然科学基金(No.81273838、81303019、81303018)
【分类号】：R245

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