弥漫性伤害抑制性控制系统在针刺抑制伤害性刺激中的作用
发布时间：2018-10-24 18:14
【摘要】：目的:观察不同强度电针对伤害性刺激的镇痛效应差异,探讨弥漫性伤害抑制性控制(DNIC)系统是否参与了电针的镇痛效应。方法:以人体和动物作为实验对象,进行四部分实验。动物行为学实验,用1mA、3mA电针强度电针针刺正常清醒大鼠两侧足三里,观察针刺前后的机械刺激诱导的缩足反射。动物C-纤维反射实验,电刺激左侧腓神经皮肤感受野测得能诱发出股二头肌肌电(E MG)的Aδ,C-纤维反射阈值。以1.2倍C-纤维反射的阈值强度作为检验刺激,分别以不同强度,电针记录电极同侧、对侧足三里,记录电针前后0-5min检验刺激诱发的EMG变化。人体dQST试验挑选符合条件的10名知情健康志愿者入组为受试者,应用温度感觉分析仪给予左侧大鱼际一个4s47℃热灸的检验刺激,记录1min电针的条件刺激对检验刺激的影响。受试者分别在电针前及电针开始后30s,1min,1min30s和2min给出疼痛评分VAS值,分析不同强度电针前后VAS值的变化。人体RⅢ反射试验,给予左侧腓肠神经刺激,得出诱发股二头肌肌电的RⅢ反射阈值,并且以1.2倍的RⅢ反射阈值作检验刺激,分别以不同强度电针两侧足三里,并记录电针前及电针结束后0-5min诱发EMG的变化。结果:(1)动物行为学实验结果与人体dQST试验结果基本一致。动物行为学实验,大鼠机械性缩足反射潜伏期是13.04±3.09(s)。阈值是13.28±3.02(g)。两种强度针刺后,大鼠机械性缩足反射潜伏期和阈值均升高(p0.05,n=10)。人体dQST试验,受试者疼痛评分VAS平均值是61.5±8.1。电针结束后,受试者疼痛评分VAS值降低,耐痛值增高(p0.05,n=10)。且电针强度越大,大鼠和人体痛阈越高。(2)动物c-纤维反射实验和人体RⅢ反射试验结果基本一致。C-纤维反射实验测得10只正常SD大鼠发生Aδ反射的阈值刺激(TAδ)为0.54±0.23mA,C-纤维反射的阈值刺激(Tc)为2.92±0.39mA。Tc、2Tc强度电针对侧足三里,在针刺结束后1min内,1.2Tc引起C反射反应的EMG减少,而0.8Ta强度电针对C反射反应的抑制效果不明显。人体RⅢ反射试验测得10名受试者诱发出RⅢ反射的阈值刺激(TrⅢ)是7.95±1.8mA,电针强度增加,对EMG的抑制效果增强。结论:条件刺激对检验刺激的抑制效应随着电针强度的增大而增强,抑制时间随着电针强度的增大而延长。因此条件刺激作为可控参数,是决定针刺镇痛的关键因素之一。引起C-纤维反射的电针强度,抑制对侧C-纤维反射的EMG反应,起到镇痛的效果。针刺异神经节段支配部位所诱导的全身性镇痛效果,弥漫性伤害性抑制控制(DNIC)参与了针刺异神经节段部位的全身性镇痛作用,也可能有其他的镇痛机制参与。
[Abstract]:Aim: to observe the analgesic effects of electroacupuncture on nociceptive stimuli with different intensities, and to explore whether the diffuse nociceptive inhibitory control (DNIC) system is involved in the analgesic effect of electroacupuncture. Methods: four parts of the experiment were carried out with human and animal as experimental objects. The animal behavior experiment was conducted to observe the reflex induced by mechanical stimulation before and after acupuncture in normal conscious rats. In animal C-fiber reflex experiment, the electrical stimulation of the left peroneal nerve skin sensing field could induce the A 未 and C-fiber reflex threshold of the biceps femoris electromyography (E MG). The threshold intensity of 1.2-fold C-fiber reflex was used as the test stimulus. The changes of EMG induced by 0-5min test before and after electroacupuncture were recorded with different intensities and electroacupuncture recording electrode ipsilateral and contralateral Zusanli respectively. Human dQST test selected 10 informed and healthy volunteers as subjects. They were given a test stimulation of 4s47 鈩，
本文编号：2292160
[Abstract]:Aim: to observe the analgesic effects of electroacupuncture on nociceptive stimuli with different intensities, and to explore whether the diffuse nociceptive inhibitory control (DNIC) system is involved in the analgesic effect of electroacupuncture. Methods: four parts of the experiment were carried out with human and animal as experimental objects. The animal behavior experiment was conducted to observe the reflex induced by mechanical stimulation before and after acupuncture in normal conscious rats. In animal C-fiber reflex experiment, the electrical stimulation of the left peroneal nerve skin sensing field could induce the A 未 and C-fiber reflex threshold of the biceps femoris electromyography (E MG). The threshold intensity of 1.2-fold C-fiber reflex was used as the test stimulus. The changes of EMG induced by 0-5min test before and after electroacupuncture were recorded with different intensities and electroacupuncture recording electrode ipsilateral and contralateral Zusanli respectively. Human dQST test selected 10 informed and healthy volunteers as subjects. They were given a test stimulation of 4s47 鈩，
本文编号：2292160
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