苗药了哥王不同炮制品乙醇提取物对小鼠的急性毒性作用比较

发布时间：2018-10-30 12:24

【摘要】：目的:比较苗药了哥王生品、不同炮制品乙醇提取物对小鼠的急性毒性,为了哥王汗渍法炮制工艺优选及其安全用药提供依据。方法:采用汗渍法对了哥王的饮片炮制30d得炮制品一,对了哥王粗粉分别炮制14、7d得炮制品二和炮制品三。以70%乙醇为溶剂,采用渗漉法对了哥王生品及不同炮制品进行提取,并对不同乙醇提取物进行小鼠急性毒性实验。结果:了哥王生品、炮制品一乙醇提取物的半数致死量(LD_(50))分别为4.05、6.65 g/kg,相当于临床70kg成人日用量的19、32倍。而了哥王炮制品二、炮制品三乙醇提取物的LD50无法测出;最大耐受量(MTD)分别为20.0、15.0 g/kg,相当于临床70 kg成人日用量的95、71倍;最大给药量(MLD)分别近似为30.0、20.0 g/kg,相当于临床70kg成人日用量的143、95倍。结论:了哥王炮制品的毒性明显低于生品,且了歌王粗粉炮制14d后毒性低于炮制7d。
[Abstract]:Objective: to compare the acute toxicity of ethanol extracts from different processed products to mice. Methods: perspiration method was used to extract the processed products for 30 days, and the crude powder for 14 ~ 7 days were processed for the second product and the processed product 3 respectively. In this paper, 70% ethanol was used as solvent to extract the crude and processed products of Wang by percolation method, and the acute toxicity test of different ethanol extracts was carried out in mice. Results: half lethal dose (LD_ _ (50) of ethanol extract was 4.05 ~ 6.65 g / kg, which was 32 times of that of clinical adult 70kg. The LD50 of the processed triethanol extract could not be detected, and the maximum tolerance (MTD) was 20.0 ~ 15.0 g / kg, which was 95 ~ 71 times that of the clinical 70 kg adult daily consumption. The maximum dose of (MLD) was approximately 30. 0 ~ 20. 0 g 路kg ~ (-1), which was equivalent to 1 43 ~ 95 times of the daily dose of clinical 70kg in adults. Conclusion: the toxicity of the processed product was obviously lower than that of the raw product, and the toxicity of the processed product was lower than that of the processed product for 7 days after 14 days of processing.
【作者单位】：贵阳中医学院药学院;天津中医药大学中药学院;国家苗药工程技术研究中心;
【基金】：国家中医药管理局中医药部门公共卫生服务项目(No.国中医药科技中药便函[2015]132号) 国家级大学生创新创业训练计划项目(No.201610662025) 贵阳中医学院基金项目(No.贵中医科院内[2016]40号) 贵州省高校工程技术研究中心建设项目(No.黔教合KY字[2012]021号)
【分类号】：R29

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