中药糖通饮、穴位埋线对糖尿病周围神经病变大鼠血清NGF、TGF-β1的影响
发布时间：2018-11-03 14:37
【摘要】：目的:通过采用内服中药(糖通饮)及穴位埋线的方法对糖尿病周围神经病变(diabetic peripheral neuropathy,DPN)大鼠进行干预,观察比较各组大鼠空腹血糖(fasting blood glucose,FBG)、血清神经生长因子(nerve growth factor,NGF)、血清转化生长因子β1(transforming growth factor-β1,TGF-β1)、坐骨神经动作电位传导速度及坐骨神经病理形态学变化,探讨糖通饮及穴位埋线治疗DPN可能的作用机理和途径,为临床治疗及优选治疗方案提供理论依据。方法:将大鼠随机分为正常组、模型组、埋线组、中药组、中药+埋线组(药埋组),通过高能量饲料喂养联合多次小剂量腹腔注射0.1%浓度链脲佐菌霉素(Streptozocin,STZ)溶液的方法诱导建立糖尿病周围神经病变大鼠模型,模型成功后根据分组进行相应治疗。经10周治疗后,检测大鼠FBG、坐骨神经动作电位传导速度及血清NGF、TGF-β1变化,并取大鼠坐骨神经观察其病理形态学变化。结果:经干预治疗后:1)各治疗组大鼠FBG值较模型组降低(P0.05或P0.01),其中药埋组下降最为明显(P0.05),其余两治疗组间比较无差异(P0.05);2)三治疗组大鼠坐骨神经动作电位传导速度较模型组均有所升高,差异具有统计学意义(P0.05),但各治疗组间神经动作电位传导速度未见明显差异(P0.05);3)各治疗组大鼠血清NGF含量均较模型组明显升高(P0.01),其中药埋组血清NGF含量升高最为明显(P0.01),中药组与穴埋组比较无显著差异(P0.05);4)三治疗组大鼠血清TGF-β1含量均低于模型组(P0.01),其中药埋组血清TGF-β1含量降低最为明显(P0.01),其它两治疗组间比较无明显差异(P0.05);5)模型组大鼠坐骨神经形态较正常组大鼠有明显改变,经治疗后,三治疗组大鼠坐骨神经形态较模型组大鼠有所改善。结论:中药糖通饮和穴位埋线均能有效治疗DPN,延缓DPN病情进展,两者合用时疗效最佳,其可能的作用途径为:通过降低血糖,改善糖代谢,增加NGF含量,降低TGF-β1含量,促进受损神经的修复,来实现延缓DPN病情进展的目的。
[Abstract]:Objective: to observe and compare the fasting blood glucose (fasting blood glucose,FBG) of diabetic peripheral neuropathy (diabetic peripheral neuropathy,DPN) rats by taking traditional Chinese medicine (Tangtong Yin) and embedding thread at acupoint. Serum nerve growth factor (nerve growth factor,NGF), serum transforming growth factor 尾 1 (transforming growth factor- 尾 1 (TGF- 尾 1), sciatic nerve action potential conduction velocity and histopathologic changes of sciatic nerve. To explore the possible mechanism and approach of Tangtong Yin and acupoint catgut embedding in the treatment of DPN, and to provide theoretical basis for clinical treatment and selective treatment. Methods: rats were randomly divided into normal group, model group, catgut embedding group and traditional Chinese medicine embedding group (drug embedding group). The rats were fed with high energy feed and intraperitoneally injected with 0.1% Streptozocin,. Rat model of diabetic peripheral neuropathy was established by STZ solution. After 10 weeks of treatment, the FBG, sciatic nerve action potential conduction velocity and serum NGF,TGF- 尾 1 were measured, and the histopathologic changes of the sciatic nerve were observed. Results: after intervention: 1) the FBG value of rats in each treatment group was lower than that in the model group (P0.05 or P0.01), and the decrease was most obvious in the traditional Chinese medicine embedding group (P0.05), but there was no difference between the other two treatment groups (P0.05). 2) the conduction velocity of sciatic nerve action potential in the three treatment groups was higher than that in the model group, the difference was statistically significant (P0.05), but there was no significant difference in the nerve action potential conduction velocity among the three treatment groups (P0.05). 3) the serum NGF content in each treatment group was significantly higher than that in the model group (P0.01), and the serum NGF content in the TCM embedding group was the most significant (P0.01), but there was no significant difference between the Chinese medicine group and the acupoint embedding group (P0.05). 4) the content of serum TGF- 尾 1 in the three treatment groups was lower than that in the model group (P0.01), and the serum TGF- 尾 1 content in the traditional Chinese medicine embedding group was the most significant (P0.01), but there was no significant difference between the other two groups (P0.05). 5) the shape of sciatic nerve in the model group was obviously changed than that in the normal group. After treatment, the shape of the sciatic nerve in the three treatment groups was improved compared with that in the model group. Conclusion: Tangtong Yin and catgut embedding at acupoints can effectively treat DPN, and delay the progression of DPN. The best therapeutic effect can be achieved when the two drugs are combined. The possible ways of their action are as follows: decreasing blood glucose, improving glucose metabolism, increasing NGF content and decreasing TGF- 尾 1 content. To promote the repair of damaged nerve to delay the progression of DPN.
【学位授予单位】：贵州医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R245
本文编号：2308079
[Abstract]:Objective: to observe and compare the fasting blood glucose (fasting blood glucose,FBG) of diabetic peripheral neuropathy (diabetic peripheral neuropathy,DPN) rats by taking traditional Chinese medicine (Tangtong Yin) and embedding thread at acupoint. Serum nerve growth factor (nerve growth factor,NGF), serum transforming growth factor 尾 1 (transforming growth factor- 尾 1 (TGF- 尾 1), sciatic nerve action potential conduction velocity and histopathologic changes of sciatic nerve. To explore the possible mechanism and approach of Tangtong Yin and acupoint catgut embedding in the treatment of DPN, and to provide theoretical basis for clinical treatment and selective treatment. Methods: rats were randomly divided into normal group, model group, catgut embedding group and traditional Chinese medicine embedding group (drug embedding group). The rats were fed with high energy feed and intraperitoneally injected with 0.1% Streptozocin,. Rat model of diabetic peripheral neuropathy was established by STZ solution. After 10 weeks of treatment, the FBG, sciatic nerve action potential conduction velocity and serum NGF,TGF- 尾 1 were measured, and the histopathologic changes of the sciatic nerve were observed. Results: after intervention: 1) the FBG value of rats in each treatment group was lower than that in the model group (P0.05 or P0.01), and the decrease was most obvious in the traditional Chinese medicine embedding group (P0.05), but there was no difference between the other two treatment groups (P0.05). 2) the conduction velocity of sciatic nerve action potential in the three treatment groups was higher than that in the model group, the difference was statistically significant (P0.05), but there was no significant difference in the nerve action potential conduction velocity among the three treatment groups (P0.05). 3) the serum NGF content in each treatment group was significantly higher than that in the model group (P0.01), and the serum NGF content in the TCM embedding group was the most significant (P0.01), but there was no significant difference between the Chinese medicine group and the acupoint embedding group (P0.05). 4) the content of serum TGF- 尾 1 in the three treatment groups was lower than that in the model group (P0.01), and the serum TGF- 尾 1 content in the traditional Chinese medicine embedding group was the most significant (P0.01), but there was no significant difference between the other two groups (P0.05). 5) the shape of sciatic nerve in the model group was obviously changed than that in the normal group. After treatment, the shape of the sciatic nerve in the three treatment groups was improved compared with that in the model group. Conclusion: Tangtong Yin and catgut embedding at acupoints can effectively treat DPN, and delay the progression of DPN. The best therapeutic effect can be achieved when the two drugs are combined. The possible ways of their action are as follows: decreasing blood glucose, improving glucose metabolism, increasing NGF content and decreasing TGF- 尾 1 content. To promote the repair of damaged nerve to delay the progression of DPN.
【学位授予单位】：贵州医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R245
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