基于色谱质谱联用技术的中医痰湿体质的代谢组学研究

发布时间：2018-11-05 15:03

【摘要】：目的:寻找中医痰湿体质血清标志性代谢物及其引起的代谢通路变化,结合糖脂代谢、胰岛素抵抗指数等指标,探讨中医痰湿体质形成、发展等各阶段的病理特征以及中医关于"肥人多痰湿"的发病机制。方法:收集正常组平和质、超重组非痰湿质和痰湿质、肥胖组痰湿质的血清样本,分析比较4组间体脂参数(体重指数BMI、腰围WC、腰臀比WHR)、血脂(TG、CHOL、HDLC、LDLC)、空腹血糖(FPG)、空腹胰岛素(FINS)、胰岛素抵抗指数(Homa-IR),并分别与中医痰湿体质积分作相关性分析。应用高效液相色谱-四级杆-飞行时间质谱联用(HPLC-Q-TOF/MS)的代谢组学技术为基础、使用多元数据统计分析寻找痰湿体质的血清差异代谢物,进一步探讨中医痰湿体质内在的病理学特征及相关疾病的发病机制。结果:BMI、WC、WHR、TG随着组别依次呈现逐渐增大的趋势,HDL-C则逐渐减小,肥胖组痰湿质的FINS、Homa-IR指数显著高于正常组平和质(P0.01);全组中医痰湿体质积分与BMI、WC、WHR、TG水平呈显著正相关(P0.05);与HDL-C水平呈显著负相关(P0.01);超重组非痰湿质显著性差异表达血清代谢物有8种,超重组痰湿质有9种,肥胖组痰湿质有9种(P0.05)。结论:痰湿质肥胖症存在着显著的体脂分布异常、脂代谢紊乱、胰岛素水平过高、高胰岛素抵抗等病理特征,提示中医痰湿体质是肥胖症的易患体质;中医痰湿体质存在显著性差异表达血清代谢物。
[Abstract]:Objective: to explore the formation of phlegm dampness constitution in traditional Chinese medicine (TCM) by looking for the changes of serum metabolites and the metabolic pathways caused by them, combining the indexes of glucose and lipid metabolism, insulin resistance index and so on. The pathological features of various stages of development and the pathogenesis of "fat people with more phlegm and dampness" in TCM. Methods: the serum samples of normal group, non-phlegm wet substance and phlegm wet substance were collected, and the body fat parameters (body mass index (BMI,), waist circumference, WC, waist-hip ratio, WHR), blood lipid (TG,CHOL,HDLC,LDLC) were analyzed and compared among the four groups. Fasting blood glucose (FPG),) fasting insulin (FINS), insulin resistance index (Homa-IR) and TCM phlegm and dampness constitution score were analyzed respectively. On the basis of high performance liquid chromatography (HPLC)-Quaternary rod-time-of-flight mass spectrometry (HPLC-Q-TOF/MS), multivariate statistical analysis was used to find the serum metabolites of phlegm-wet constitution. To further explore the internal pathological characteristics of phlegm and dampness constitution of TCM and the pathogenesis of related diseases. Results: BMI,WC,WHR,TG showed an increasing trend with the group gradually increasing, while HDL-C decreased gradually. The FINS,Homa-IR index of phlegm wet substance in obesity group was significantly higher than that in normal group (P0.01). The scores of phlegm and dampness in the whole group were positively correlated with the level of BMI,WC,WHR,TG (P0.05), negatively correlated with the level of HDL-C (P0.01). There were 8 kinds of serum metabolites, 9 kinds of superrecombinant phlegm wet substance and 9 kinds of phlegm wet substance in obesity group (P0.05). Conclusion: phlegm dampness obesity has some pathological features, such as abnormal body fat distribution, disorder of lipid metabolism, high insulin level and high insulin resistance, which suggests that phlegm dampness constitution of traditional Chinese medicine is prone to obesity. There are significant differences in the expression of serum metabolites in TCM phlegm and dampness constitution.
【作者单位】：浙江中医药大学基础医学院;
【基金】：浙江省中医药管理局项目(2012ZB027)
【分类号】：R229

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