电针不同穴位对颈部切口痛大鼠颈段背根节内卫星胶质细胞活动的影响

发布时间：2018-11-07 10:15

【摘要】：目的:通过观察电针对甲状腺区切口痛大鼠颈背根神经节(DRG)内卫星胶质细胞(SGCs)活性的影响,探讨针刺缓解术后痛或针刺镇痛行甲状腺手术的机制。方法:SD大鼠随机分为正常组、模型组、扶突组、合谷-内关组、足三里-阳陵泉组,每组20只。除正常组外,沿大鼠颈正中线做一纵行切口并反复分离刺激,制作甲状腺区切口痛模型。各治疗组分别电针双侧"扶突""合谷-内关""足三里-阳陵泉"穴,1次/d,连续3d。检测大鼠切口部位热痛阈;用免疫荧光、Real-time PCR、ELISA和蛋白免疫印迹法分别检测大鼠脊髓颈(C)2-C 6DRGs内SGCs标记物胶质纤维酸性蛋白(GFAP)、connexin 43(Cx 43)免疫荧光强度和白细胞介素-1β(IL-1β)、肿瘤坏死因子-α(TNF-α)、IL-6mRNA和蛋白表达量以及Cx 43蛋白表达量。结果 :模型组大鼠颈部的热痛阈较正常组明显缩短(P0.05);扶突组和合谷-内关组大鼠热痛阈均较模型组和足三里-阳陵泉组显著延长(P0.05)。DRGs内GFAP免疫荧光强度及mRNA表达水平与IL-1β、TNF-α、IL-6mRNA和蛋白表达量及Cx 43免疫荧光强度与蛋白表达量在模型组均明显上调(P0.05);与模型组比较,电针双侧"扶突""合谷-内关"穴后GFAP免疫荧光强度及mRNA表达水平与IL-1β、TNF-α、IL-6mRNA表达水平和Cx 43免疫荧光强度与蛋白表达量,扶突组IL-1β、TNF-α与合谷-内关组IL-6蛋白表达量均明显下调(P0.05),足三里-阳陵泉组除IL-1β、TNF-αmRNA外,多数指标变化不明显(P0.05)。结论:电针"扶突""合谷-内关"穴可缓解大鼠颈部切口痛,该作用可能与其下调C 2-C 6DRGs内SGCs活性,减少促炎细胞因子的释放,减弱SGCs间信息交流密切相关。
[Abstract]:Objective: to observe the effect of electroacupuncture on (SGCs) activity of satellite glia in (DRG) of cervical dorsal root ganglion (DRG) of rats with incision pain in thyroid region, and to explore the mechanism of relieving postoperative pain or acupuncture analgesia by acupuncture. Methods: SD rats were randomly divided into normal group, model group, FUP group, Hegu-Neiguan group and Zusanli-Yanglingquan group with 20 rats in each group. In addition to the normal group, a longitudinal incision was made along the median line of the neck of the rat and stimulated repeatedly to make the model of incision pain in the thyroid region. Each treatment group was treated with electroacupuncture at Zusanli Yangling Quan (Zusanli-Yanglingquan) once a day for 3 days. The thermal pain threshold of the incision was measured in rats. Detection of glial fibrillary acidic protein (GFAP), in rat spinal cord (C) 2-C 6DRGs by immunofluorescence, Real-time PCR,ELISA and Western blot Connexin 43 (Cx 43) immunofluorescence intensity, interleukin-1 尾 (IL-1 尾), tumor necrosis factor- 伪 (TNF- 伪), IL-6mRNA and protein expression and Cx 43 protein expression. Results: the thermal pain threshold of the model group was significantly shorter than that of the normal group (P0.05). Compared with model group and Zusanli-Yanglingquan group, the thermal pain threshold in FUP group and Hegu Neiguan group was significantly longer than that in model group and Zusanli-Yanglingquan group (P0.05) GFAP immunofluorescence intensity and mRNA expression level and IL-1 尾, TNF- 伪 were significantly increased in FUP group and Hegu Neiguan group. The expression of IL-6mRNA and protein, the immunofluorescence intensity and protein expression of Cx 43 were significantly up-regulated in the model group (P0.05). Compared with the model group, GFAP immunofluorescence intensity and mRNA expression level, IL-1 尾, TNF- 伪, IL-6mRNA expression level and Cx 43 immunofluorescence intensity and protein expression level after electroacupuncture at Hegu and Neiguan acupoints of both sides were compared, IL-1 尾, IL-1 尾 in FUT group were significantly higher than those in control group. The expression of TNF- 伪 and IL-6 protein in Hegu-Neiguan group were significantly down-regulated (P0.05), except IL-1 尾 and TNF- 伪 mRNA in Zusanli-Yanglingquan group (P0.05). Conclusion: electroacupuncture at Hegu Neiguan point can relieve the pain of neck incision in rats, which may be related to its down-regulation of SGCs activity in C2-C 6DRGs, the reduction of inflammatory cytokines release and the decrease of information exchange among SGCs.
【作者单位】：中国中医科学院针灸研究所;长春中医药大学针灸推拿学院;北京中医药大学针灸推拿学院;
【基金】：国家自然科学基金青年基金项目(No.81303055);国家自然科学基金面上项目(No.81273833) 科技部国家重点基础研究发展计划“973”项目(No.2014CB 543103、2013CB 531904) 中国中医科学院针灸研究所科研业务费自主选题(No.ZZ 201711006)
【分类号】：R245.97

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