寿胎丸含药血清对自然流产患者滋养层细胞凋亡相关蛋白表达的影响

发布时间：2018-11-08 17:00

【摘要】：目的探讨寿胎丸防治自然流产的可能作用机制。方法体外分离培养自然流产患者滋养层细胞,并用不同浓度(5%、10%、20%)寿胎丸含药血清干预24h(寿胎丸含药血清组),并以空白血清及地屈孕酮含药血清分别作为空白对照(空白血清组)及阳性对照(地屈孕酮含药血清组),每个浓度3个复孔。检测各组不同药物浓度血清干预后滋养层细胞总凋亡比例及细胞凋亡相关蛋白Bcl-2、Bax蛋白表达水平。结果与空白血清组比较,寿胎丸含药血清组和地屈孕酮含药血清组各浓度药物血清干预后能降低滋养层细胞总凋亡比例,上调Bcl-2蛋白水平,在浓度为10%和20%的药物血清干预后下调Bax蛋白水平(P0.05),并且呈一定的剂量依赖性(P0.05)。结论寿胎丸含药血清呈剂量依赖性减少自然流产患者滋养层细胞凋亡,可能与上调Bcl-2蛋白、下调Bax蛋白有关。
[Abstract]:Objective to explore the possible mechanism of Shoutai pills in preventing and treating spontaneous abortion. Methods the trophoblast cells of patients with spontaneous abortion were isolated and cultured in vitro, and treated with different concentrations (5101010%) of Shoutai pills for 24 h (group B). Blank serum and diproprogesterone serum were used as blank control (blank serum group) and positive control group (diproprogesterone containing serum group) respectively. The ratio of total apoptosis in trophoblast cells and the expression of apoptosis-related protein (Bcl-2,Bax) in trophoblastic cells were measured after serum intervention with different drug concentrations in each group. Results compared with the blank serum group, the total apoptosis rate of trophoblastic cells and the level of Bcl-2 protein were up-regulated after the intervention of each concentration of drug serum of Shoufeiwan and Diproprogesterone. The concentration of 10% and 20% of the drug serum decreased the level of Bax protein (P0.05), and showed a dose-dependent (P0.05). Conclusion the serum of Shoufeiwan can decrease the apoptosis of trophoblastic cells in a dose-dependent manner, which may be related to the up-regulation of Bcl-2 protein and down-regulation of Bax protein.
【作者单位】：南方医科大学附属深圳市妇幼保健院;
【基金】：广东省中医药局“建设中医药强省”科研项目(20152058)
【分类号】：R271.9

【相似文献】