脾阳虚模型大鼠“运”“化”功能的增龄性变化

发布时间：2018-11-18 15:03

【摘要】：目的:探讨脾阳虚模型大鼠"运""化"功能的增龄性变化。方法:将新出生40只Wistar大鼠,体质量(5.6±0.5)g,采用肩胛骨间棕色脂肪组织切除术及高脂饮食隔日寒冷环境刺激造模,术后随机分为8周龄模型组、16周龄模型组、32周龄模型组、64周龄模型组4组,每组10只。另外40只大鼠用来设立相对应的同周龄的对照组4组,每组10只。到了相应的周龄后,检测胃肠动力学指标,采用免疫组化检测胃促生长素表达,酶联免疫吸附法和放射免疫法检测血清胃促生长素、胃泌素、瘦素、神经肽Y水平。结果:32周龄模型组、64周龄模型组分别与同周龄对照组比较,在胃肠动力学指标差异有统计学意义(P0.05或P0.01),血清胃泌素、瘦素、神经肽Y水平差异也均有统计学意义(P0.05或P0.01),免疫组化检测结果显示胃促生长素表达有增龄性递减趋势。结论:脾阳虚模型大鼠"运""化"功能随增龄减弱,与胃促生长素调节胃肠运动和调控胃泌素、瘦素、神经肽Y水平通路机制有关。
[Abstract]:Objective: to study the aging changes of the function of "transport" and "transformation" of spleen yang deficiency model rats. Methods: forty newly born Wistar rats with body mass of (5.6 卤0.5) g were randomly divided into 8-week-old model group and 16-week-old model group. 32 weeks old model group, 64 weeks old model group 4 groups, each group 10 rats. The other 40 rats were used to set up corresponding control group of the same age, 10 rats in each group. The gastrin, leptin and neuropeptide Y levels in serum were detected by immunohistochemistry, enzyme linked immunosorbent assay (Elisa) and radioimmunoassay (RIA). Results: compared with the control group of the same age, there were significant differences in gastrointestinal dynamic indexes between the 32 week old model group and 64 week old model group (P0.05 or P0.01). The differences of neuropeptide Y levels were statistically significant (P0.05 or P0.01). The results of immunohistochemistry showed that the expression of gastrin had an age-related decreasing trend. Conclusion: the function of "transport" and "transformation" of spleen yang deficiency model rats decreases with age, which is related to the regulation of gastrin, leptin and neuropeptide Y level pathway by gastric growth-promoting hormone.
【作者单位】：右江民族医学院基础医学院;广西中医药大学基础医学院;右江民族医学院临床医学院;
【基金】：广西自然科学基金项目(2015GXNSFAA139190) 广西教育厅科研立项项目(201106LX443)
【分类号】：R228;R-332

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