藏药印度獐芽菜石油醚部位化学成分及体外活性研究
发布时间：2018-11-21 11:19
【摘要】：采用各种柱色谱以及重结晶等方法对印度獐芽菜石油醚萃取部位进行化学成分研究。通过理化性质和波谱数据分析鉴定了15个化合物,包括8个釫酮,2个三萜,1个裂环烯醚萜,1个香豆素和3个其他类化合物,分别为1,8-二羟基-3,5-二甲氧基釫酮(1)、1-羟基-3,7,8-三甲氧基釫酮(2)、1,8-二羟基-3,5,7-三甲氧基釫酮(3)、1-羟基-3,5,7,8-四甲氧基釫酮(4)、1,5,8-三羟基-3-甲氧基釫酮(5)、1-羟基-3,7-二甲氧基釫酮(6)、1,8-二羟基-3,7-二甲氧基釫酮(7)、1-羟基-3,5-二甲氧基釫酮(8)、古柯二醇(9)、齐墩果酸(10)、(±)-龙胆内酯(11)、东莨菪内酯(12)、芥子醛(13)、丁香醛(14)、β-谷甾醇(15)。其中化合物3,4,9,11~14为首次从该植物中分离得到,化合物9和12为首次从獐芽菜属中发现。对5个量大釫酮1,2,5,7,8进行体外活性测试,结果显示其在最高浓度50μmol·L~(-1)对胰腺癌细胞SW1990和Bx PC-3的增殖和过氧化氢(H_2O_2)诱导的人脐静脉融合细胞EA.hy926氧化应激损伤均无明显作用。
[Abstract]:The chemical constituents of petroleum ether extracted from Aeurotus sinensis were studied by column chromatography and recrystallization. Fifteen compounds including 8 ketones, 2 triterpenoids, 1 cleft ring ether terpene, 1 coumarin and 3 other compounds were identified by physical and chemical properties and spectral data analysis. The results were as follows: 1 (1), (1), (1), (1), (2), (1), (3), (3), (3), (3), (3), respectively, (1), (2), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), respectively, (7) -tetramethoxy ketone (4), 1-trihydroxy-3-methoxy ketone (5), 1-hydroxy-3-trihydroxy-3-dimethoxy ketone (6), 1-dihydroxy-3-trimethoxy ketone (7), 1-hydroxy-3-dimethoxy ketone (8), coca diol (9), oleanolic acid (10), (卤) -gentian lactone (11), scopolactone (12), mustard aldehyde (13), eugenaldehyde (14), 尾 -sitosterol (15). Among them, compound 3O4C4C9A1111C14 was isolated from the plant for the first time, and compounds 9 and 12 were found for the first time from the genus Aeurourotus, and compounds 9 and 12 were isolated from the plant for the first time. In vitro activity tests were carried out for 5 doses of 1, 2, 2, 5, 5, 5, 5, 1, 5, 5, 1, 5, 5, 5, 5 The results showed that at the highest concentration of 50 渭 mol L ~ (-1), the proliferation of pancreatic cancer cells SW1990 and Bx PC-3 and the oxidative stress injury induced by hydrogen peroxide (H_2O_2) in human umbilical vein fusion cells were not obvious.
【作者单位】： 南京中医药大学附属中西医结合医院;安徽中医药大学药学院;南京医科大学药学院;江苏省农业种质资源保护与利用平台;
【基金】：江苏省药学会奥赛康临床药学研究基金项目(210303) 南京医科大学药学院大学生创新训练计划项目(2017YXDC02)
【分类号】：R29
本文编号：2346809
[Abstract]:The chemical constituents of petroleum ether extracted from Aeurotus sinensis were studied by column chromatography and recrystallization. Fifteen compounds including 8 ketones, 2 triterpenoids, 1 cleft ring ether terpene, 1 coumarin and 3 other compounds were identified by physical and chemical properties and spectral data analysis. The results were as follows: 1 (1), (1), (1), (1), (2), (1), (3), (3), (3), (3), (3), respectively, (1), (2), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), respectively, (7) -tetramethoxy ketone (4), 1-trihydroxy-3-methoxy ketone (5), 1-hydroxy-3-trihydroxy-3-dimethoxy ketone (6), 1-dihydroxy-3-trimethoxy ketone (7), 1-hydroxy-3-dimethoxy ketone (8), coca diol (9), oleanolic acid (10), (卤) -gentian lactone (11), scopolactone (12), mustard aldehyde (13), eugenaldehyde (14), 尾 -sitosterol (15). Among them, compound 3O4C4C9A1111C14 was isolated from the plant for the first time, and compounds 9 and 12 were found for the first time from the genus Aeurourotus, and compounds 9 and 12 were isolated from the plant for the first time. In vitro activity tests were carried out for 5 doses of 1, 2, 2, 5, 5, 5, 5, 1, 5, 5, 1, 5, 5, 5, 5 The results showed that at the highest concentration of 50 渭 mol L ~ (-1), the proliferation of pancreatic cancer cells SW1990 and Bx PC-3 and the oxidative stress injury induced by hydrogen peroxide (H_2O_2) in human umbilical vein fusion cells were not obvious.
【作者单位】： 南京中医药大学附属中西医结合医院;安徽中医药大学药学院;南京医科大学药学院;江苏省农业种质资源保护与利用平台;
【基金】：江苏省药学会奥赛康临床药学研究基金项目(210303) 南京医科大学药学院大学生创新训练计划项目(2017YXDC02)
【分类号】：R29
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