电针对脑缺血大鼠丘脑腹后外侧核神经生长因子和生长休止特定蛋白7表达的影响

发布时间：2018-11-26 18:05

【摘要】：目的:观察电针对局灶性脑缺血大鼠丘脑腹后外侧核(VPL)神经生长因子(NGF)和生长休止特定蛋白7(Gas 7)表达的影响,探讨电针治疗局灶性脑缺血继发性损伤的可能机制。方法:采用随机数字表法将48只SD大鼠分为正常组、假手术组、模型组和缺血后电针治疗组(简称电针组),每组12只。采用线栓法复制右侧大脑中动脉栓塞模型。电针组电针"百会"和左侧"足三里"穴,每天1次,每次30 min,连续21d。免疫组织化学法和Western blot法检测大鼠右侧VPL中NGF和Gas 7的表达;尼氏染色法观察VPL神经元形态。结果:尼氏染色结果显示,正常组及假手术组右侧VPL结构清晰完整,胞核居中,核仁清楚;模型组VPL神经元染色较深,细胞核固缩;电针组神经细胞形态与正常组接近。免疫组织化学和Western blot检测结果一致,假手术组右侧VPL中NGF和Gas 7蛋白的表达与正常组比较差异无统计学意义(P0.05);与正常组比较,模型组缺血侧VPL中NGF和Gas 7蛋白表达明显升高(P0.05);与模型组比较,电针组缺血侧VPL中NGF和Gas 7蛋白表达显著升高(P0.05)。结论:局灶性脑缺血大鼠缺血侧VPL中NGF和Gas 7的表达增强,可能参与了机体代偿性恢复的某些过程;电针可上调缺血侧VPL中NGF和Gas 7的表达,可能是电针改善脑缺血继发性丘脑损害的机制之一。
[Abstract]:Objective: to observe the effect of electroacupuncture on the expression of (VPL) nerve growth factor (NGF) and growth rest specific protein 7 (Gas 7) in ventrolateral nucleus of thalamus of rats with focal cerebral ischemia. To explore the possible mechanism of electroacupuncture in treating secondary injury of focal cerebral ischemia. Methods: 48 SD rats were randomly divided into normal group, sham operation group, model group and electroacupuncture group after ischemia (12 rats in each group). The right middle cerebral artery (MCA) embolization model was established by thread embolization. In the electroacupuncture group, the points of Baihui and Zusanli on the left side were treated once a day for 30 min, for 21 days. The expression of NGF and Gas 7 in the right VPL of rats was detected by immunohistochemistry and Western blot, and the morphology of VPL neurons was observed by Nissl staining. Results: the results of Nissl staining showed that the right VPL structure of normal group and sham operation group was clear and complete, the nucleus was in the middle, the nucleolus was clear, the VPL neurons in the model group were stained deeply and the nucleus was pyknosis, and the morphology of nerve cells in the electroacupuncture group was close to that in the normal group. The expression of NGF and Gas 7 protein in VPL of sham-operated group was not significantly different from that of normal group (P0.05). Compared with the normal group, the expression of NGF and Gas 7 protein in VPL of the model group was significantly higher than that in the model group (P0.05); compared with the model group, the expression of NGF and Gas 7 protein in the VPL of the electroacupuncture group was significantly higher (P0.05). Conclusion: the increased expression of NGF and Gas 7 in VPL of rats with focal cerebral ischemia may be involved in the process of compensatory recovery. Electroacupuncture can up-regulate the expression of NGF and Gas 7 in VPL of ischemic side, which may be one of the mechanisms of electroacupuncture in ameliorating the secondary thalamic damage after cerebral ischemia.
【作者单位】：皖南医学院人体解剖学教研室;
【基金】：皖南医学院中青年科研基金项目(No.WK 201509);皖南医学院校重点科研项目培育基金项目(No.WK 2016Z09) 安徽省青年自然科学基金(No.1608085QH 223) 安徽省名师工作室(No.2014msgzs 152)
【分类号】：R245.97

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