不同生长期采集的蒙药森登化学成分比较研究
发布时间：2018-11-26 17:40
【摘要】：目的:该文照高效液相色谱法(HPLC)同时测定32批蒙药材森登中表儿茶素、二氢槲皮素、杨梅素3个成份的含量测定及建立森登HPLC指纹图谱,并进行单体分离实验,为森登的质量控制及继续研究提供依据。方法:含量测定及指纹图谱研究:该方法色谱条件为ODS C18色谱柱(4.6×300mm,5μm),以乙腈(B)和0.1%的醋酸(A)为流动相,检测波长为274nm,梯度洗脱,流速1mL/min,进样量5μL,柱温30℃。单体分离研究:取森登的粗粉,加95%的乙醇回流提取3次,低温(40℃)回收乙醇浓缩加少量蒸馏水,依次加同体积石油醚、二氯甲烷、正丁醇萃取,取3个萃取液。二氯甲烷萃取液凝胶柱色谱进行分离。对分离得到的化合物进行光谱结构鉴定。结果:含量测定中显示表儿茶素、二氢槲皮素、杨梅素3个被测组分达到基线分离;在线性范围内具有良好的线性关系,相关系数均大于0.999;平均回收率分别为99.61%、99.73%、99.91%。不同来源的27批森登进行HPLC指纹图谱研究,与共有模式之间具有良好的相似性,相似度大于0.99。HPLC指纹图谱研究中发现23种共有峰,其中表儿茶素、二氢槲皮素、二氢杨梅素、杨梅素、槲皮素、柑橘素6个成份与标准品对照已确定。单体分离实验中,分离出儿茶素、二氢杨梅素、1-O-甲基-D肌-肌醇3个化合物,其中三号化合物为森登里首次分离得到的新化合物。结论:该研究不仅作为蒙药森登质量控制的基础,还进一步道地药材的采收,加工、有效成分的分析开发利用提供研究基础。
[Abstract]:Objective: to determine the content of epigallocatechin, dihydroquercetin and myricetin in 32 batches of Mongolian medicinal herbs by (HPLC) and to establish the HPLC fingerprint of Senden. It provides the basis for the quality control and continuous research of Senden. Methods: the chromatographic conditions of the method were as follows: ODS C18 column (4.6 脳 300 mm ~ (-1) 5 渭 m),) with acetonitrile (B) and 0.1% (A) acetic acid as mobile phase, detection wavelength at 274 nm, gradient elution, flow rate of 1 mL / min. The sample size was 5 渭 L and the column temperature was 30 鈩，
本文编号：2359200
[Abstract]:Objective: to determine the content of epigallocatechin, dihydroquercetin and myricetin in 32 batches of Mongolian medicinal herbs by (HPLC) and to establish the HPLC fingerprint of Senden. It provides the basis for the quality control and continuous research of Senden. Methods: the chromatographic conditions of the method were as follows: ODS C18 column (4.6 脳 300 mm ~ (-1) 5 渭 m),) with acetonitrile (B) and 0.1% (A) acetic acid as mobile phase, detection wavelength at 274 nm, gradient elution, flow rate of 1 mL / min. The sample size was 5 渭 L and the column temperature was 30 鈩，
本文编号：2359200
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