电针百会、神庭穴对脑缺血再灌注大鼠学习记忆能力及海马5-羟色胺1A受体表达的影响

发布时间：2018-11-28 08:00

【摘要】：目的探讨电针百会、神庭穴对脑缺血再灌注(MCAO/R)大鼠学习记忆的影响及其可能的作用机制。方法将90只健康雄性Sprague-Dawley大鼠随机分为假手术组(n=18)和手术组(n=72)。手术组参照线栓法制备MCAO/R大鼠模型。将符合纳入标准的54只手术组大鼠随机分为模型组(n=18)、电针组(n=18)和非穴组(n=18)。电针组电针百会、神庭穴共14 d。小动物磁共振扫描检测脑梗死体积;Morris水迷宫测试检测学习记忆能力;免疫组化检测5-羟色胺1A(5-HT_(1A))受体的定位和表达。结果干预前各组大鼠脑梗死体积无显著性差异(F=1.678,P0.05)。干预后,与模型组和非穴组相比,电针组脑梗死体积显著减小(P0.001)。Morris水迷宫测试中,电针组较模型组和非穴组逃避潜伏期显著缩短(P0.001),穿越平台次数增加(P0.05)。5-HT_(1A)受体在缺血侧海马CA1、CA3和DG区均出现表达,电针组表达量较模型组和非穴组均降低(P0.05)。非穴组各项指标与模型组比较均无显著性差异(P0.05)。结论电针百会、神庭穴可有效改善MCAO/R大鼠的学习记忆能力,其机制可能与调节海马区5-HT_(1A)受体表达有关。
[Abstract]:Objective to investigate the effect of electroacupuncture at Baihui and Shenting on learning and memory in rats with cerebral ischemia reperfusion (MCAO/R) and its possible mechanism. Methods 90 healthy male Sprague-Dawley rats were randomly divided into sham operation group (n = 18) and operation group (n = 72). The MCAO/R rat model was established in the operation group according to the method of thread embolization. Fifty-four rats in operation group were randomly divided into three groups: model group (n = 18), electroacupuncture group (n = 18) and non-point group (n = 18). In the electroacupuncture group, the acupoints of Shenting and Baihui were 14 days. Morris water maze test was used to detect the ability of learning and memory, and the localization and expression of 5-hydroxytryptamine 1A (5-HT _ (1A) receptor were detected by immunohistochemistry. Results there was no significant difference in cerebral infarction volume between the groups before intervention (P 0.05). After intervention, compared with model group and non-point group, the volume of cerebral infarction in electroacupuncture group decreased significantly (P0.001). Morris water maze test, electroacupuncture group than model group and non-point group significantly shortened the escape latency (P0. 001). The expression of 5-HT _ (1A) receptor was found in CA1,CA3 and DG of ischemic hippocampus, and the expression of 5-HT _ (1A) receptor in electroacupuncture group was lower than that in model group and non-point group (P0.05). There was no significant difference between the non-point group and the model group (P0.05). Conclusion electroacupuncture at Baihui and Shenting points can effectively improve the learning and memory ability of MCAO/R rats, and its mechanism may be related to the regulation of 5-HT _ (1A) receptor expression in hippocampus.
【作者单位】：福建中医药大学康复医学院;福建省康复技术协同创新中心;康复医疗技术国家地方联合工程研究中心;国家中医药管理局中医康复研究中心;
【基金】：国家自然科学基金项目(No.81403450)
【分类号】：R245

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