针刺对端粒酶敲除小鼠大脑海马区、皮质区Trk-B信号调节激酶表达的影响

发布时间：2018-11-28 08:56

【摘要】：目的:探讨临床常用的不同针刺方法,手针、电针干预对端粒酶敲除小鼠大脑海马区、皮质区Trk-B信号调节激酶表达的影响。方法:端粒酶敲除小鼠按随机数字表法分为手针组、电针组、空白对照组各6只。电针组使用一对华佗牌不锈钢针具(0.3 mm×0.25 mm)对双侧足三里进行针刺干预,交替频率,强度0.5~1.2 mA,幅度2/100 HZ,每日30 min,连续干预7 d。手针组使用华佗牌不锈钢针具(0.3 mm×0.25 mm)对双侧足三里进行针刺干预,每5 min捻转1次,每次30 min,空白对照组不做任何干预。采用Western blot法检测大脑海马和皮质区域Trk-B的表达情况。结果:手针组小鼠在干预后自主活动程度较空白组均有所增加,海马区电针组与空白组对照组比较Trk-B表达明显增高;手针组与空白对照组比较Trk-B表达明显增高;但在皮质区电针组与手针组比较Trk-B表达明显降低;电针组与空白对照组比较表达明显降低。结论:电针对不同脑区Trk-B表达的影响不同,电针可促进海马区Trk-B的表达,相同的穴位刺激电针和手针的作用机制不同,可能与酪氨酸B(Trk-B)信号调节激酶表达有关。
[Abstract]:Aim: to investigate the effects of hand acupuncture and electroacupuncture intervention on the expression of Trk-B signal regulated kinase in hippocampus and cortex of telomerase knockout mice. Methods: telomerase knockout mice were randomly divided into hand acupuncture group, electroacupuncture group and blank control group. In the electroacupuncture group, a pair of stainless steel needles of Huatuo brand (0.3 mm 脳 0.25 mm) were used to treat bilateral Zusanli acupuncture for 7 consecutive days. The alternating frequency, intensity of 0.5 mA, and 0.5 mA, amplitude of 2 / 100 HZ, 30 min, per day for 7 days. The hand acupuncture group was treated with Huatuo stainless steel needle (0.3 mm 脳 0. 25 mm) for bilateral Zusanli acupuncture intervention, twirled once every 5 min, without any intervention in 30 min, blank control group. The expression of Trk-B in hippocampus and cortex was detected by Western blot method. Results: the degree of autonomous activity in the hand acupuncture group was higher than that in the blank group, the expression of Trk-B in the hippocampal electroacupuncture group was significantly higher than that in the blank group, and the expression of Trk-B in the hand acupuncture group was significantly higher than that in the blank control group. However, the expression of Trk-B in the electroacupuncture group was significantly lower than that in the hand acupuncture group, and the expression of Trk-B in the electroacupuncture group was significantly lower than that in the blank control group. Conclusion: the effect of electroacupuncture on the expression of Trk-B in different brain regions is different. Electroacupuncture can promote the expression of Trk-B in hippocampal area, and the mechanism of stimulation of electroacupuncture and hand acupuncture at the same point is different. Tyrosine B (Trk-B) signal-regulated kinase expression may be involved.
【作者单位】：福建中医药大学针灸学院;
【基金】：福建省自然科学基金资助项目(2017J01540)-基于端粒酶活性相关的针刺抗衰老效应对Terc-/-小鼠海马神经元保护作用研究福建省资助省属高校科研专项JK类(JK2016018/JAT160221)-自发热温灸针对慢性神经痛模型大鼠脊髓疼痛调控时效特征的研究
【分类号】：R245

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